Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media
Human mesenchymal stem cells (hMSCs) are presently being evaluated for their therapeutic potential in clinical studies to treat various diseases, disorders, and injuries. To date, early-phase studies have indicated that the use of both autologous and allogeneic hMSCs appear to be safe; however, effi...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/123030 |
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| author | Sunghoon Jung Krishna M. Panchalingam Lawrence Rosenberg Leo A. Behie |
| author_facet | Sunghoon Jung Krishna M. Panchalingam Lawrence Rosenberg Leo A. Behie |
| author_sort | Sunghoon Jung |
| collection | DOAJ |
| description | Human mesenchymal stem cells (hMSCs) are presently being evaluated for their therapeutic potential in clinical studies to treat various diseases, disorders, and injuries. To date, early-phase studies have indicated that the use of both autologous and allogeneic hMSCs appear to be safe; however, efficacy has not been demonstrated in recent late-stage clinical trials. Optimized cell bioprocessing protocols may enhance the efficacy as well as safety of hMSC therapeutics. Classical media used for generating hMSCs are typically supplemented with ill-defined supplements such as fetal bovine serum (FBS) or human-sourced alternatives. Ideally, culture media are desired to have well-defined serum-free formulations that support the efficient production of hMSCs while maintaining their therapeutic and differentiation capacity. Towards this objective, we review here current cell culture media for hMSCs and discuss medium development strategies. |
| format | Article |
| id | doaj-art-72aa27f8b6794716b1bb7778263de6e7 |
| institution | DOAJ |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-72aa27f8b6794716b1bb7778263de6e72025-08-20T03:19:03ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/123030123030Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free MediaSunghoon Jung0Krishna M. Panchalingam1Lawrence Rosenberg2Leo A. Behie3Pharmaceutical Production Research Facility (PPRF), Schulich School of Engineering, University of Calgary, Calgary, AB, T2N 1N4, CanadaPharmaceutical Production Research Facility (PPRF), Schulich School of Engineering, University of Calgary, Calgary, AB, T2N 1N4, CanadaDepartment of Surgery, McGill University, Montreal, QC, H3G 1A4, CanadaPharmaceutical Production Research Facility (PPRF), Schulich School of Engineering, University of Calgary, Calgary, AB, T2N 1N4, CanadaHuman mesenchymal stem cells (hMSCs) are presently being evaluated for their therapeutic potential in clinical studies to treat various diseases, disorders, and injuries. To date, early-phase studies have indicated that the use of both autologous and allogeneic hMSCs appear to be safe; however, efficacy has not been demonstrated in recent late-stage clinical trials. Optimized cell bioprocessing protocols may enhance the efficacy as well as safety of hMSC therapeutics. Classical media used for generating hMSCs are typically supplemented with ill-defined supplements such as fetal bovine serum (FBS) or human-sourced alternatives. Ideally, culture media are desired to have well-defined serum-free formulations that support the efficient production of hMSCs while maintaining their therapeutic and differentiation capacity. Towards this objective, we review here current cell culture media for hMSCs and discuss medium development strategies.http://dx.doi.org/10.1155/2012/123030 |
| spellingShingle | Sunghoon Jung Krishna M. Panchalingam Lawrence Rosenberg Leo A. Behie Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media Stem Cells International |
| title | Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media |
| title_full | Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media |
| title_fullStr | Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media |
| title_full_unstemmed | Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media |
| title_short | Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media |
| title_sort | ex vivo expansion of human mesenchymal stem cells in defined serum free media |
| url | http://dx.doi.org/10.1155/2012/123030 |
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