Enhanced assessment of contractile dynamics in Drosophila hearts
The Drosophila heart has gained considerable traction as a model of cardiac development and physiology. Previously we described a semiautomatic optical heartbeat analysis (SOHA) method for quantifying functional parameters from the fly heart that facilitated its use as an organ system and disease mo...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2015-02-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/000114255 |
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| _version_ | 1850152910746288128 |
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| author | Anthony Cammarato Shawn Ocorr Karen Ocorr |
| author_facet | Anthony Cammarato Shawn Ocorr Karen Ocorr |
| author_sort | Anthony Cammarato |
| collection | DOAJ |
| description | The Drosophila heart has gained considerable traction as a model of cardiac development and physiology. Previously we described a semiautomatic optical heartbeat analysis (SOHA) method for quantifying functional parameters from the fly heart that facilitated its use as an organ system and disease model. Here we present an extensively rewritten version of the original SOHA program that takes advantage of additional information contained in high-speed videos of beating hearts. Program updates allow more precise quantification of cardiac contractions, increase the signal-to-noise ratio, and reduce the overall cost and time required to analyze recordings. This new SOHA version permits relatively rapid and highly accurate determination of subphases of contraction and relaxation. Importantly, the improved functionality enables the calculation of novel physiological data, suggesting that the fly model system may also be practical for screening drugs and alleles that modulate cardiac repolarization and force production. |
| format | Article |
| id | doaj-art-72a3e229841e414d899aebd83f33169f |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2015-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-72a3e229841e414d899aebd83f33169f2025-08-20T02:25:51ZengTaylor & Francis GroupBioTechniques0736-62051940-98182015-02-01582778010.2144/000114255Enhanced assessment of contractile dynamics in Drosophila heartsAnthony Cammarato0Shawn Ocorr1Karen Ocorr21Sanford-Burnham Medical Research Institute, La Jolla, CA1Sanford-Burnham Medical Research Institute, La Jolla, CA1Sanford-Burnham Medical Research Institute, La Jolla, CAThe Drosophila heart has gained considerable traction as a model of cardiac development and physiology. Previously we described a semiautomatic optical heartbeat analysis (SOHA) method for quantifying functional parameters from the fly heart that facilitated its use as an organ system and disease model. Here we present an extensively rewritten version of the original SOHA program that takes advantage of additional information contained in high-speed videos of beating hearts. Program updates allow more precise quantification of cardiac contractions, increase the signal-to-noise ratio, and reduce the overall cost and time required to analyze recordings. This new SOHA version permits relatively rapid and highly accurate determination of subphases of contraction and relaxation. Importantly, the improved functionality enables the calculation of novel physiological data, suggesting that the fly model system may also be practical for screening drugs and alleles that modulate cardiac repolarization and force production.https://www.future-science.com/doi/10.2144/000114255cardiacDrosophilacontraction velocityrelative forcemyosinthapsigargin |
| spellingShingle | Anthony Cammarato Shawn Ocorr Karen Ocorr Enhanced assessment of contractile dynamics in Drosophila hearts BioTechniques cardiac Drosophila contraction velocity relative force myosin thapsigargin |
| title | Enhanced assessment of contractile dynamics in Drosophila hearts |
| title_full | Enhanced assessment of contractile dynamics in Drosophila hearts |
| title_fullStr | Enhanced assessment of contractile dynamics in Drosophila hearts |
| title_full_unstemmed | Enhanced assessment of contractile dynamics in Drosophila hearts |
| title_short | Enhanced assessment of contractile dynamics in Drosophila hearts |
| title_sort | enhanced assessment of contractile dynamics in drosophila hearts |
| topic | cardiac Drosophila contraction velocity relative force myosin thapsigargin |
| url | https://www.future-science.com/doi/10.2144/000114255 |
| work_keys_str_mv | AT anthonycammarato enhancedassessmentofcontractiledynamicsindrosophilahearts AT shawnocorr enhancedassessmentofcontractiledynamicsindrosophilahearts AT karenocorr enhancedassessmentofcontractiledynamicsindrosophilahearts |