Impact of olive oil and different animal fats on tissue lipid profiles of C57PL/6N mice fed non-atherogenic diet

Abstract Animal fats are widely used in nutrition despite their different chemical composition. Consumption of these fats may be linked to the incidence of certain diseases, depending on the type and quantity of fatty acids present; however, this hypothesis has not been confirmed. Thus, we fed C57PL...

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Main Authors: Mohsen A. Zommara, Mohamad B. Atta, Mei-Chu Hung, Katsumi Imaizumi, Mohamed Ghanimah
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-14995-w
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author Mohsen A. Zommara
Mohamad B. Atta
Mei-Chu Hung
Katsumi Imaizumi
Mohamed Ghanimah
author_facet Mohsen A. Zommara
Mohamad B. Atta
Mei-Chu Hung
Katsumi Imaizumi
Mohamed Ghanimah
author_sort Mohsen A. Zommara
collection DOAJ
description Abstract Animal fats are widely used in nutrition despite their different chemical composition. Consumption of these fats may be linked to the incidence of certain diseases, depending on the type and quantity of fatty acids present; however, this hypothesis has not been confirmed. Thus, we fed C57PL/6 N mice various animal fats, namely cow tallow, buffalo tallow, mutton tallow, chicken fat, and cow or buffalo milk fat, to examine their effects on growth, serum, liver, and adipose tissue lipid profiles, as well as aortic valve lesions. The results showed that the type of dietary fat had no remarkable effect on food intake or the relative weights of the spleen and liver. Except for the chicken fat-fed group, the mice fed milk fat exhibited higher feed efficiency, body weight gain, and adipose tissue content than the other groups. The diets supplemented with olive oil or chicken fat resulted in the lowest plasma total cholesterol and LDL levels, while the group fed milk fat had the highest concentration of plasma HDL and triglycerides in both plasma and adipose tissue. Although the animal fats used in this study showed non-atherogenic effects, chicken fat demonstrated better biological and nutritional properties than other animal fats.
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institution Kabale University
issn 2045-2322
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publishDate 2025-08-01
publisher Nature Portfolio
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series Scientific Reports
spelling doaj-art-72a12c1d71d64f07adc95917176ce1662025-08-20T03:42:22ZengNature PortfolioScientific Reports2045-23222025-08-0115111310.1038/s41598-025-14995-wImpact of olive oil and different animal fats on tissue lipid profiles of C57PL/6N mice fed non-atherogenic dietMohsen A. Zommara0Mohamad B. Atta1Mei-Chu Hung2Katsumi Imaizumi3Mohamed Ghanimah4Department of Dairy Science, Faculty of Agriculture, Kafrelsheikh UniversityDepartment of Food Science and Technology, Faculty of Agriculture, Tanta University, Sibirbay CampusDepartment of Applied Life Science and Health, Chia Nan University of Pharmacy and ScienceGraduate School of Bioresource and Bioenvironmental Sciences, Department of Bioscience and Biotechnology, Kyushu UniversityDepartment of Dairy Science, Faculty of Agriculture, Kafrelsheikh UniversityAbstract Animal fats are widely used in nutrition despite their different chemical composition. Consumption of these fats may be linked to the incidence of certain diseases, depending on the type and quantity of fatty acids present; however, this hypothesis has not been confirmed. Thus, we fed C57PL/6 N mice various animal fats, namely cow tallow, buffalo tallow, mutton tallow, chicken fat, and cow or buffalo milk fat, to examine their effects on growth, serum, liver, and adipose tissue lipid profiles, as well as aortic valve lesions. The results showed that the type of dietary fat had no remarkable effect on food intake or the relative weights of the spleen and liver. Except for the chicken fat-fed group, the mice fed milk fat exhibited higher feed efficiency, body weight gain, and adipose tissue content than the other groups. The diets supplemented with olive oil or chicken fat resulted in the lowest plasma total cholesterol and LDL levels, while the group fed milk fat had the highest concentration of plasma HDL and triglycerides in both plasma and adipose tissue. Although the animal fats used in this study showed non-atherogenic effects, chicken fat demonstrated better biological and nutritional properties than other animal fats.https://doi.org/10.1038/s41598-025-14995-wOlive oilCow tallowBuffalo tallowMutton tallowChicken fatMilk fat
spellingShingle Mohsen A. Zommara
Mohamad B. Atta
Mei-Chu Hung
Katsumi Imaizumi
Mohamed Ghanimah
Impact of olive oil and different animal fats on tissue lipid profiles of C57PL/6N mice fed non-atherogenic diet
Scientific Reports
Olive oil
Cow tallow
Buffalo tallow
Mutton tallow
Chicken fat
Milk fat
title Impact of olive oil and different animal fats on tissue lipid profiles of C57PL/6N mice fed non-atherogenic diet
title_full Impact of olive oil and different animal fats on tissue lipid profiles of C57PL/6N mice fed non-atherogenic diet
title_fullStr Impact of olive oil and different animal fats on tissue lipid profiles of C57PL/6N mice fed non-atherogenic diet
title_full_unstemmed Impact of olive oil and different animal fats on tissue lipid profiles of C57PL/6N mice fed non-atherogenic diet
title_short Impact of olive oil and different animal fats on tissue lipid profiles of C57PL/6N mice fed non-atherogenic diet
title_sort impact of olive oil and different animal fats on tissue lipid profiles of c57pl 6n mice fed non atherogenic diet
topic Olive oil
Cow tallow
Buffalo tallow
Mutton tallow
Chicken fat
Milk fat
url https://doi.org/10.1038/s41598-025-14995-w
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