Oxytocin signaling in mouse taste buds.
<h4>Background</h4>The neuropeptide, oxytocin (OXT), acts on brain circuits to inhibit food intake. Mutant mice lacking OXT (OXT knockout) overconsume salty and sweet (i.e. sucrose, saccharin) solutions. We asked if OXT might also act on taste buds via its receptor, OXTR.<h4>Method...
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Public Library of Science (PLoS)
2010-08-01
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| author | Michael S Sinclair Isabel Perea-Martinez Gennady Dvoryanchikov Masahide Yoshida Katsuhiko Nishimori Stephen D Roper Nirupa Chaudhari |
| author_facet | Michael S Sinclair Isabel Perea-Martinez Gennady Dvoryanchikov Masahide Yoshida Katsuhiko Nishimori Stephen D Roper Nirupa Chaudhari |
| author_sort | Michael S Sinclair |
| collection | DOAJ |
| description | <h4>Background</h4>The neuropeptide, oxytocin (OXT), acts on brain circuits to inhibit food intake. Mutant mice lacking OXT (OXT knockout) overconsume salty and sweet (i.e. sucrose, saccharin) solutions. We asked if OXT might also act on taste buds via its receptor, OXTR.<h4>Methodology/principal findings</h4>Using RT-PCR, we detected the expression of OXTR in taste buds throughout the oral cavity, but not in adjacent non-taste lingual epithelium. By immunostaining tissues from OXTR-YFP knock-in mice, we found that OXTR is expressed in a subset of Glial-like (Type I) taste cells, and also in cells on the periphery of taste buds. Single-cell RT-PCR confirmed this cell-type assignment. Using Ca2+ imaging, we observed that physiologically appropriate concentrations of OXT evoked [Ca2+]i mobilization in a subset of taste cells (EC50 approximately 33 nM). OXT-evoked responses were significantly inhibited by the OXTR antagonist, L-371,257. Isolated OXT-responsive taste cells were neither Receptor (Type II) nor Presynaptic (Type III) cells, consistent with our immunofluorescence observations. We also investigated the source of OXT peptide that may act on taste cells. Both RT-PCR and immunostaining suggest that the OXT peptide is not produced in taste buds or in their associated nerves. Finally, we also examined the morphology of taste buds from mice that lack OXTR. Taste buds and their constituent cell types appeared very similar in mice with two, one or no copies of the OXTR gene.<h4>Conclusions/significance</h4>We conclude that OXT elicits Ca2+ signals via OXTR in murine taste buds. OXT-responsive cells are most likely a subset of Glial-like (Type I) taste cells. OXT itself is not produced locally in taste tissue and is likely delivered through the circulation. Loss of OXTR does not grossly alter the morphology of any of the cell types contained in taste buds. Instead, we speculate that OXT-responsive Glial-like (Type I) taste bud cells modulate taste signaling and afferent sensory output. Such modulation would complement central pathways of appetite regulation that employ circulating homeostatic and satiety signals. |
| format | Article |
| id | doaj-art-7292fa5f88ef4f50ad37cc5d9716cbf0 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2010-08-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-7292fa5f88ef4f50ad37cc5d9716cbf02025-08-20T02:31:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-08-0158e1198010.1371/journal.pone.0011980Oxytocin signaling in mouse taste buds.Michael S SinclairIsabel Perea-MartinezGennady DvoryanchikovMasahide YoshidaKatsuhiko NishimoriStephen D RoperNirupa Chaudhari<h4>Background</h4>The neuropeptide, oxytocin (OXT), acts on brain circuits to inhibit food intake. Mutant mice lacking OXT (OXT knockout) overconsume salty and sweet (i.e. sucrose, saccharin) solutions. We asked if OXT might also act on taste buds via its receptor, OXTR.<h4>Methodology/principal findings</h4>Using RT-PCR, we detected the expression of OXTR in taste buds throughout the oral cavity, but not in adjacent non-taste lingual epithelium. By immunostaining tissues from OXTR-YFP knock-in mice, we found that OXTR is expressed in a subset of Glial-like (Type I) taste cells, and also in cells on the periphery of taste buds. Single-cell RT-PCR confirmed this cell-type assignment. Using Ca2+ imaging, we observed that physiologically appropriate concentrations of OXT evoked [Ca2+]i mobilization in a subset of taste cells (EC50 approximately 33 nM). OXT-evoked responses were significantly inhibited by the OXTR antagonist, L-371,257. Isolated OXT-responsive taste cells were neither Receptor (Type II) nor Presynaptic (Type III) cells, consistent with our immunofluorescence observations. We also investigated the source of OXT peptide that may act on taste cells. Both RT-PCR and immunostaining suggest that the OXT peptide is not produced in taste buds or in their associated nerves. Finally, we also examined the morphology of taste buds from mice that lack OXTR. Taste buds and their constituent cell types appeared very similar in mice with two, one or no copies of the OXTR gene.<h4>Conclusions/significance</h4>We conclude that OXT elicits Ca2+ signals via OXTR in murine taste buds. OXT-responsive cells are most likely a subset of Glial-like (Type I) taste cells. OXT itself is not produced locally in taste tissue and is likely delivered through the circulation. Loss of OXTR does not grossly alter the morphology of any of the cell types contained in taste buds. Instead, we speculate that OXT-responsive Glial-like (Type I) taste bud cells modulate taste signaling and afferent sensory output. Such modulation would complement central pathways of appetite regulation that employ circulating homeostatic and satiety signals.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0011980&type=printable |
| spellingShingle | Michael S Sinclair Isabel Perea-Martinez Gennady Dvoryanchikov Masahide Yoshida Katsuhiko Nishimori Stephen D Roper Nirupa Chaudhari Oxytocin signaling in mouse taste buds. PLoS ONE |
| title | Oxytocin signaling in mouse taste buds. |
| title_full | Oxytocin signaling in mouse taste buds. |
| title_fullStr | Oxytocin signaling in mouse taste buds. |
| title_full_unstemmed | Oxytocin signaling in mouse taste buds. |
| title_short | Oxytocin signaling in mouse taste buds. |
| title_sort | oxytocin signaling in mouse taste buds |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0011980&type=printable |
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