Nitration of β-Lactoglobulin but Not of Ovomucoid Enhances Anaphylactic Responses in Food Allergic Mice.
<h4>Background</h4>We revealed in previous studies that nitration of food proteins reduces the risk of de novo sensitization in a murine food allergy model. In contrast, in situations with preformed specific IgE antibodies, in vitro experiments suggested an increased capacity of effector...
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0126279&type=printable |
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| author | Susanne C Diesner Cornelia Schultz Chloé Ackaert Gertie J Oostingh Anna Ondracek Caroline Stremnitzer Josef Singer Denise Heiden Franziska Roth-Walter Judit Fazekas Vera E Assmann Erika Jensen-Jarolim Hanno Stutz Albert Duschl Eva Untersmayr |
| author_facet | Susanne C Diesner Cornelia Schultz Chloé Ackaert Gertie J Oostingh Anna Ondracek Caroline Stremnitzer Josef Singer Denise Heiden Franziska Roth-Walter Judit Fazekas Vera E Assmann Erika Jensen-Jarolim Hanno Stutz Albert Duschl Eva Untersmayr |
| author_sort | Susanne C Diesner |
| collection | DOAJ |
| description | <h4>Background</h4>We revealed in previous studies that nitration of food proteins reduces the risk of de novo sensitization in a murine food allergy model. In contrast, in situations with preformed specific IgE antibodies, in vitro experiments suggested an increased capacity of effector cell activation by nitrated food proteins.<h4>Objective</h4>The aim of this study was to investigate the influence of protein nitration on the effector phase of food allergy.<h4>Design</h4>BALB/c mice were immunized intraperitoneally (i.p.) with the milk allergen β-lactoglobulin (BLG) or the egg allergen ovomucoid (OVM), followed by intragastric (i.g.) gavages to induce a strong local inflammatory response and allergen-specific antibodies. Subsequently, naïve and allergic mice were intravenously (i.v.) challenged with untreated, sham-nitrated or nitrated BLG or OVM. Anaphylaxis was monitored by measuring core body temperature and determination of mouse mast cell protease-1 (mMCP-1) levels in blood.<h4>Results</h4>A significant drop of body temperature accompanied with significantly elevated concentrations of the anaphylaxis marker mMCP-1 were only observed in BLG allergic animals challenged with nitrated BLG and not in OVM allergic mice challenged with nitrated OVM. SDS-PAGE and circular dichroism analysis of the differentially modified allergens revealed an effect of nitration on the secondary protein structure exclusively for BLG together with enhanced protein aggregation.<h4>Conclusion</h4>Our data suggest that nitration affects differently the food allergens BLG and OVM. In the case of BLG, structural changes favored dimerization possibly explaining the increased anaphylactic reactivity in BLG allergic animals. |
| format | Article |
| id | doaj-art-727cfd9a3a26441ca70ea80372f46133 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-727cfd9a3a26441ca70ea80372f461332025-08-20T03:46:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012627910.1371/journal.pone.0126279Nitration of β-Lactoglobulin but Not of Ovomucoid Enhances Anaphylactic Responses in Food Allergic Mice.Susanne C DiesnerCornelia SchultzChloé AckaertGertie J OostinghAnna OndracekCaroline StremnitzerJosef SingerDenise HeidenFranziska Roth-WalterJudit FazekasVera E AssmannErika Jensen-JarolimHanno StutzAlbert DuschlEva Untersmayr<h4>Background</h4>We revealed in previous studies that nitration of food proteins reduces the risk of de novo sensitization in a murine food allergy model. In contrast, in situations with preformed specific IgE antibodies, in vitro experiments suggested an increased capacity of effector cell activation by nitrated food proteins.<h4>Objective</h4>The aim of this study was to investigate the influence of protein nitration on the effector phase of food allergy.<h4>Design</h4>BALB/c mice were immunized intraperitoneally (i.p.) with the milk allergen β-lactoglobulin (BLG) or the egg allergen ovomucoid (OVM), followed by intragastric (i.g.) gavages to induce a strong local inflammatory response and allergen-specific antibodies. Subsequently, naïve and allergic mice were intravenously (i.v.) challenged with untreated, sham-nitrated or nitrated BLG or OVM. Anaphylaxis was monitored by measuring core body temperature and determination of mouse mast cell protease-1 (mMCP-1) levels in blood.<h4>Results</h4>A significant drop of body temperature accompanied with significantly elevated concentrations of the anaphylaxis marker mMCP-1 were only observed in BLG allergic animals challenged with nitrated BLG and not in OVM allergic mice challenged with nitrated OVM. SDS-PAGE and circular dichroism analysis of the differentially modified allergens revealed an effect of nitration on the secondary protein structure exclusively for BLG together with enhanced protein aggregation.<h4>Conclusion</h4>Our data suggest that nitration affects differently the food allergens BLG and OVM. In the case of BLG, structural changes favored dimerization possibly explaining the increased anaphylactic reactivity in BLG allergic animals.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0126279&type=printable |
| spellingShingle | Susanne C Diesner Cornelia Schultz Chloé Ackaert Gertie J Oostingh Anna Ondracek Caroline Stremnitzer Josef Singer Denise Heiden Franziska Roth-Walter Judit Fazekas Vera E Assmann Erika Jensen-Jarolim Hanno Stutz Albert Duschl Eva Untersmayr Nitration of β-Lactoglobulin but Not of Ovomucoid Enhances Anaphylactic Responses in Food Allergic Mice. PLoS ONE |
| title | Nitration of β-Lactoglobulin but Not of Ovomucoid Enhances Anaphylactic Responses in Food Allergic Mice. |
| title_full | Nitration of β-Lactoglobulin but Not of Ovomucoid Enhances Anaphylactic Responses in Food Allergic Mice. |
| title_fullStr | Nitration of β-Lactoglobulin but Not of Ovomucoid Enhances Anaphylactic Responses in Food Allergic Mice. |
| title_full_unstemmed | Nitration of β-Lactoglobulin but Not of Ovomucoid Enhances Anaphylactic Responses in Food Allergic Mice. |
| title_short | Nitration of β-Lactoglobulin but Not of Ovomucoid Enhances Anaphylactic Responses in Food Allergic Mice. |
| title_sort | nitration of β lactoglobulin but not of ovomucoid enhances anaphylactic responses in food allergic mice |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0126279&type=printable |
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