Multi-targeted olink proteomics analyses of cerebrospinal fluid from patients with aneurysmal subarachnoid hemorrhage
Abstract Background The complexity of delayed cerebral ischemia (DCI) after aneurysmal subarachnoid hemorrhage (aSAH) may require the simultaneous analysis of variant types of protein biomarkers to describe it more accurately. In this study, we analyzed for the first time the alterations of cerebros...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-11-01
|
| Series: | Proteome Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12953-024-00236-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850056014640971776 |
|---|---|
| author | Rui Ding Liquan Wu Shanshan Wei Haoran Lu Xiaohong Qin Xizhi Liu Yanhua Wang Wen Liu Huibing Li Baochang Luo Teng Xie Zhibiao Chen |
| author_facet | Rui Ding Liquan Wu Shanshan Wei Haoran Lu Xiaohong Qin Xizhi Liu Yanhua Wang Wen Liu Huibing Li Baochang Luo Teng Xie Zhibiao Chen |
| author_sort | Rui Ding |
| collection | DOAJ |
| description | Abstract Background The complexity of delayed cerebral ischemia (DCI) after aneurysmal subarachnoid hemorrhage (aSAH) may require the simultaneous analysis of variant types of protein biomarkers to describe it more accurately. In this study, we analyzed for the first time the alterations of cerebrospinal fluid (CSF) proteins in patients with aSAH by multi-targeted Olink proteomics, aiming to reveal the pathophysiology of DCI and provide insights into the diagnosis and treatment of aSAH. Methods Six aSAH patients and six control patients were selected, and CSF samples were analyzed by Olink Proteomics (including 96-neurology panel and 96-inflammation panel) based on Proximity Extension Assay (PEA). Differentially expressed proteins (DEPs) were acquired and bioinformatics analysis was performed. Results PCA analysis revealed better intra- and inter-group reproducibility of CSF samples in the control and aSAH groups. 23 neurology-related and 31 inflammation-relevant differential proteins were identified. In the neurology panel, compared to controls, the up-regulated proteins in the CSF of SAH patients predominantly included macrophage scavenger receptor 1 (MSR1), siglec-1, siglec-9, cathepsin C (CTSC), cathepsin S (CTSS), etc. Meanwhile, in the inflammation group, the incremental proteins mainly contained interleukin-6 (IL-6), MCP-1, CXCL10, CXCL-9, TRAIL, etc. Cluster analysis exhibited significant differences in differential proteins between the two groups. GO function enrichment analysis hinted that the differential proteins pertinent to neurology in the CSF of SAH patients were mainly involved in the regulation of defense response, vesicle-mediated transport and regulation of immune response; while the differential proteins related to inflammation were largely connected with the cellular response to chemokine, response to chemokine and chemokine-mediated signaling pathway. Additionally, in the neurology panel, KEGG enrichment analysis indicated that the differential proteins were significantly enriched in the phagosome, apoptosis and microRNAs in cancer pathway. And in the inflammation panel, the differential proteins were mainly enriched in the chemokine signaling pathway, viral protein interaction with cytokine and cytokine receptor and toll-like receptor signaling pathway. Conclusions These identified differential proteins reveal unique pathophysiological characteristics secondary to aSAH. Further characterization of these proteins and aberrant pathways in future research could enable their application as potential therapeutic targets and biomarkers for DCI after aSAH. |
| format | Article |
| id | doaj-art-7267a5e470fb4a7fa2eef2fc37be4ae5 |
| institution | DOAJ |
| issn | 1477-5956 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj-art-7267a5e470fb4a7fa2eef2fc37be4ae52025-08-20T02:51:48ZengBMCProteome Science1477-59562024-11-0122111510.1186/s12953-024-00236-xMulti-targeted olink proteomics analyses of cerebrospinal fluid from patients with aneurysmal subarachnoid hemorrhageRui Ding0Liquan Wu1Shanshan Wei2Haoran Lu3Xiaohong Qin4Xizhi Liu5Yanhua Wang6Wen Liu7Huibing Li8Baochang Luo9Teng Xie10Zhibiao Chen11Department of Neurosurgery, Renmin Hospital of Wuhan UniversityDepartment of Neurosurgery, Renmin Hospital of Wuhan UniversityDepartment of Oncology, Wuchang Hospital Affiliated to Wuhan University of Science and TechnologyDepartment of Neurosurgery, Renmin Hospital of Wuhan UniversityDepartment of Neurosurgery, Renmin Hospital of Wuhan UniversityDepartment of Neurosurgery, Renmin Hospital of Wuhan UniversityDepartment of Neurosurgery, Hanchuan Renmin HospitalDepartment of Neurosurgery, Hanchuan Renmin HospitalDepartment of Neurosurgery, Hanchuan Renmin HospitalDepartment of Neurosurgery, Hanchuan Renmin HospitalDepartment of Neurosurgery, Hanchuan Renmin HospitalDepartment of Neurosurgery, Renmin Hospital of Wuhan UniversityAbstract Background The complexity of delayed cerebral ischemia (DCI) after aneurysmal subarachnoid hemorrhage (aSAH) may require the simultaneous analysis of variant types of protein biomarkers to describe it more accurately. In this study, we analyzed for the first time the alterations of cerebrospinal fluid (CSF) proteins in patients with aSAH by multi-targeted Olink proteomics, aiming to reveal the pathophysiology of DCI and provide insights into the diagnosis and treatment of aSAH. Methods Six aSAH patients and six control patients were selected, and CSF samples were analyzed by Olink Proteomics (including 96-neurology panel and 96-inflammation panel) based on Proximity Extension Assay (PEA). Differentially expressed proteins (DEPs) were acquired and bioinformatics analysis was performed. Results PCA analysis revealed better intra- and inter-group reproducibility of CSF samples in the control and aSAH groups. 23 neurology-related and 31 inflammation-relevant differential proteins were identified. In the neurology panel, compared to controls, the up-regulated proteins in the CSF of SAH patients predominantly included macrophage scavenger receptor 1 (MSR1), siglec-1, siglec-9, cathepsin C (CTSC), cathepsin S (CTSS), etc. Meanwhile, in the inflammation group, the incremental proteins mainly contained interleukin-6 (IL-6), MCP-1, CXCL10, CXCL-9, TRAIL, etc. Cluster analysis exhibited significant differences in differential proteins between the two groups. GO function enrichment analysis hinted that the differential proteins pertinent to neurology in the CSF of SAH patients were mainly involved in the regulation of defense response, vesicle-mediated transport and regulation of immune response; while the differential proteins related to inflammation were largely connected with the cellular response to chemokine, response to chemokine and chemokine-mediated signaling pathway. Additionally, in the neurology panel, KEGG enrichment analysis indicated that the differential proteins were significantly enriched in the phagosome, apoptosis and microRNAs in cancer pathway. And in the inflammation panel, the differential proteins were mainly enriched in the chemokine signaling pathway, viral protein interaction with cytokine and cytokine receptor and toll-like receptor signaling pathway. Conclusions These identified differential proteins reveal unique pathophysiological characteristics secondary to aSAH. Further characterization of these proteins and aberrant pathways in future research could enable their application as potential therapeutic targets and biomarkers for DCI after aSAH.https://doi.org/10.1186/s12953-024-00236-xAneurysmal subarachnoid hemorrhageOlink proteomicsNeurologyInflammationBioinformatics analysis |
| spellingShingle | Rui Ding Liquan Wu Shanshan Wei Haoran Lu Xiaohong Qin Xizhi Liu Yanhua Wang Wen Liu Huibing Li Baochang Luo Teng Xie Zhibiao Chen Multi-targeted olink proteomics analyses of cerebrospinal fluid from patients with aneurysmal subarachnoid hemorrhage Proteome Science Aneurysmal subarachnoid hemorrhage Olink proteomics Neurology Inflammation Bioinformatics analysis |
| title | Multi-targeted olink proteomics analyses of cerebrospinal fluid from patients with aneurysmal subarachnoid hemorrhage |
| title_full | Multi-targeted olink proteomics analyses of cerebrospinal fluid from patients with aneurysmal subarachnoid hemorrhage |
| title_fullStr | Multi-targeted olink proteomics analyses of cerebrospinal fluid from patients with aneurysmal subarachnoid hemorrhage |
| title_full_unstemmed | Multi-targeted olink proteomics analyses of cerebrospinal fluid from patients with aneurysmal subarachnoid hemorrhage |
| title_short | Multi-targeted olink proteomics analyses of cerebrospinal fluid from patients with aneurysmal subarachnoid hemorrhage |
| title_sort | multi targeted olink proteomics analyses of cerebrospinal fluid from patients with aneurysmal subarachnoid hemorrhage |
| topic | Aneurysmal subarachnoid hemorrhage Olink proteomics Neurology Inflammation Bioinformatics analysis |
| url | https://doi.org/10.1186/s12953-024-00236-x |
| work_keys_str_mv | AT ruiding multitargetedolinkproteomicsanalysesofcerebrospinalfluidfrompatientswithaneurysmalsubarachnoidhemorrhage AT liquanwu multitargetedolinkproteomicsanalysesofcerebrospinalfluidfrompatientswithaneurysmalsubarachnoidhemorrhage AT shanshanwei multitargetedolinkproteomicsanalysesofcerebrospinalfluidfrompatientswithaneurysmalsubarachnoidhemorrhage AT haoranlu multitargetedolinkproteomicsanalysesofcerebrospinalfluidfrompatientswithaneurysmalsubarachnoidhemorrhage AT xiaohongqin multitargetedolinkproteomicsanalysesofcerebrospinalfluidfrompatientswithaneurysmalsubarachnoidhemorrhage AT xizhiliu multitargetedolinkproteomicsanalysesofcerebrospinalfluidfrompatientswithaneurysmalsubarachnoidhemorrhage AT yanhuawang multitargetedolinkproteomicsanalysesofcerebrospinalfluidfrompatientswithaneurysmalsubarachnoidhemorrhage AT wenliu multitargetedolinkproteomicsanalysesofcerebrospinalfluidfrompatientswithaneurysmalsubarachnoidhemorrhage AT huibingli multitargetedolinkproteomicsanalysesofcerebrospinalfluidfrompatientswithaneurysmalsubarachnoidhemorrhage AT baochangluo multitargetedolinkproteomicsanalysesofcerebrospinalfluidfrompatientswithaneurysmalsubarachnoidhemorrhage AT tengxie multitargetedolinkproteomicsanalysesofcerebrospinalfluidfrompatientswithaneurysmalsubarachnoidhemorrhage AT zhibiaochen multitargetedolinkproteomicsanalysesofcerebrospinalfluidfrompatientswithaneurysmalsubarachnoidhemorrhage |