Physiological and Biochemical Vascular Reactivity Parameters of Angiotensin II and the Action of Biased Agonist TRV023
Vascular reactivity experiments using isolated aortic rings have been widely used as a model for physiological and pharmacological studies since the early sixties. Here, we suggest several parameters that the researcher should pay attention to when investigating angiotensin II in their experimental...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Advances in Pharmacological and Pharmaceutical Sciences |
| Online Access: | http://dx.doi.org/10.1155/2020/3092721 |
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| author | Marcos André Soares Leal Thanisia de Almeida João Guilherme Torres Luciene Cristina Gastalho Campos Elisardo Corral Vasquez Valério Garrone Barauna |
| author_facet | Marcos André Soares Leal Thanisia de Almeida João Guilherme Torres Luciene Cristina Gastalho Campos Elisardo Corral Vasquez Valério Garrone Barauna |
| author_sort | Marcos André Soares Leal |
| collection | DOAJ |
| description | Vascular reactivity experiments using isolated aortic rings have been widely used as a model for physiological and pharmacological studies since the early sixties. Here, we suggest several parameters that the researcher should pay attention to when investigating angiotensin II in their experimental models. Angiotensin II is one of the active peptides of the renin-angiotensin system and exerts its effect through the AT1 and AT2 receptors. Some studies seek to understand the effects of angiotensin II receptors at the vascular level by using vascular reactivity experiments. However, because of the large number of variations, there are only a handful of reactivity studies that seek to use this method. Thus, the objective of this study was to standardize experimental methods with angiotensin II, through vascular reactivity protocols. For this, variables such as basal tension, concentration interval, single concentration, curve concentration response, and multiple experiments using the same aortic ring were developed using the technique of vascular reactivity in an organ bath. This is the first study that has standardized the vascular reactivity protocol. In addition, we demonstrated the effects of TRV023-biased ligand of the AT1R at vascular sites. |
| format | Article |
| id | doaj-art-7261eae0a9ac4db5b98488bbfa9729b4 |
| institution | OA Journals |
| issn | 2633-4690 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological and Pharmaceutical Sciences |
| spelling | doaj-art-7261eae0a9ac4db5b98488bbfa9729b42025-08-20T02:06:23ZengWileyAdvances in Pharmacological and Pharmaceutical Sciences2633-46902020-01-01202010.1155/2020/30927213092721Physiological and Biochemical Vascular Reactivity Parameters of Angiotensin II and the Action of Biased Agonist TRV023Marcos André Soares Leal0Thanisia de Almeida1João Guilherme Torres2Luciene Cristina Gastalho Campos3Elisardo Corral Vasquez4Valério Garrone Barauna5Laboratory of Translational Physiology, Federal University of Espirito, Vitória, Espirito Santo, BrazilLaboratory of Molecular Physiology, Federal University of Espírito Santo, Vitória, Espirito Santo, BrazilPostgraduate Program in Biochemistry, Federal University of the Pampa, Uruguaiana, RS, BrazilDepartment of Biological Science, Santa Cruz State University, Ilheus, BA, BrazilLaboratory of Translational Physiology, Federal University of Espirito, Vitória, Espirito Santo, BrazilLaboratory of Molecular Physiology, Federal University of Espírito Santo, Vitória, Espirito Santo, BrazilVascular reactivity experiments using isolated aortic rings have been widely used as a model for physiological and pharmacological studies since the early sixties. Here, we suggest several parameters that the researcher should pay attention to when investigating angiotensin II in their experimental models. Angiotensin II is one of the active peptides of the renin-angiotensin system and exerts its effect through the AT1 and AT2 receptors. Some studies seek to understand the effects of angiotensin II receptors at the vascular level by using vascular reactivity experiments. However, because of the large number of variations, there are only a handful of reactivity studies that seek to use this method. Thus, the objective of this study was to standardize experimental methods with angiotensin II, through vascular reactivity protocols. For this, variables such as basal tension, concentration interval, single concentration, curve concentration response, and multiple experiments using the same aortic ring were developed using the technique of vascular reactivity in an organ bath. This is the first study that has standardized the vascular reactivity protocol. In addition, we demonstrated the effects of TRV023-biased ligand of the AT1R at vascular sites.http://dx.doi.org/10.1155/2020/3092721 |
| spellingShingle | Marcos André Soares Leal Thanisia de Almeida João Guilherme Torres Luciene Cristina Gastalho Campos Elisardo Corral Vasquez Valério Garrone Barauna Physiological and Biochemical Vascular Reactivity Parameters of Angiotensin II and the Action of Biased Agonist TRV023 Advances in Pharmacological and Pharmaceutical Sciences |
| title | Physiological and Biochemical Vascular Reactivity Parameters of Angiotensin II and the Action of Biased Agonist TRV023 |
| title_full | Physiological and Biochemical Vascular Reactivity Parameters of Angiotensin II and the Action of Biased Agonist TRV023 |
| title_fullStr | Physiological and Biochemical Vascular Reactivity Parameters of Angiotensin II and the Action of Biased Agonist TRV023 |
| title_full_unstemmed | Physiological and Biochemical Vascular Reactivity Parameters of Angiotensin II and the Action of Biased Agonist TRV023 |
| title_short | Physiological and Biochemical Vascular Reactivity Parameters of Angiotensin II and the Action of Biased Agonist TRV023 |
| title_sort | physiological and biochemical vascular reactivity parameters of angiotensin ii and the action of biased agonist trv023 |
| url | http://dx.doi.org/10.1155/2020/3092721 |
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