Systemic Administration of the Phytochemical, Myricetin, Attenuates the Excitability of Rat Nociceptive Secondary Trigeminal Neurons
While the modulation of the excitatory and inhibitory neuronal transmission by the phytochemical flavonoid, myricetin (MYR), has been noted in the nervous system, the way in which MYR affects the excitability of nociceptive sensory neurons in vivo remains to be established. This study aimed to explo...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/5/1019 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850225582365736960 |
|---|---|
| author | Sana Yamaguchi Risako Chida Syogo Utugi Yukito Sashide Mamoru Takeda |
| author_facet | Sana Yamaguchi Risako Chida Syogo Utugi Yukito Sashide Mamoru Takeda |
| author_sort | Sana Yamaguchi |
| collection | DOAJ |
| description | While the modulation of the excitatory and inhibitory neuronal transmission by the phytochemical flavonoid, myricetin (MYR), has been noted in the nervous system, the way in which MYR affects the excitability of nociceptive sensory neurons in vivo remains to be established. This study aimed to explore whether administering MYR intravenously, in acute doses, to rats, diminishes the excitability of SpVc wide-dynamic range (WDR) spinal trigeminal nucleus caudalis (SpVc) neurons in response to nociceptive and non-nociceptive mechanical stimulation in vivo. Recordings of extracellular single units were obtained from SpVc neurons when orofacial mechanical stimulation was applied to anesthetized rats. The average firing rate of SpVc WDR neurons, to both non-noxious and noxious mechanical stimuli, was significantly and dose-dependently inhibited by MYR (1–5 mM, intravenously), and the maximum reversible inhibition of the discharge frequency, for both non-noxious and noxious mechanical stimuli, occurred within 5–10 min. The suppressive effects of MYR continued for about 20 min. These findings indicate that an acute, intravenous administration of MYR reduces the SpVc nociceptive transmission, likely through the inhibition of the CaV channels and by activating the Kv channels. Therefore, MYR might be utilized as a treatment for trigeminal nociceptive pain, without causing side effects. |
| format | Article |
| id | doaj-art-72606f4f710f4e1c975d202384fab4b6 |
| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-72606f4f710f4e1c975d202384fab4b62025-08-20T02:05:19ZengMDPI AGMolecules1420-30492025-02-01305101910.3390/molecules30051019Systemic Administration of the Phytochemical, Myricetin, Attenuates the Excitability of Rat Nociceptive Secondary Trigeminal NeuronsSana Yamaguchi0Risako Chida1Syogo Utugi2Yukito Sashide3Mamoru Takeda4Laboratory of Food and Physiological Sciences, Department of Life and Food Sciences, School of Life and Environmental Sciences, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara 252-5201, Kanagawa, JapanLaboratory of Food and Physiological Sciences, Department of Life and Food Sciences, School of Life and Environmental Sciences, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara 252-5201, Kanagawa, JapanLaboratory of Food and Physiological Sciences, Department of Life and Food Sciences, School of Life and Environmental Sciences, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara 252-5201, Kanagawa, JapanLaboratory of Food and Physiological Sciences, Department of Life and Food Sciences, School of Life and Environmental Sciences, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara 252-5201, Kanagawa, JapanLaboratory of Food and Physiological Sciences, Department of Life and Food Sciences, School of Life and Environmental Sciences, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara 252-5201, Kanagawa, JapanWhile the modulation of the excitatory and inhibitory neuronal transmission by the phytochemical flavonoid, myricetin (MYR), has been noted in the nervous system, the way in which MYR affects the excitability of nociceptive sensory neurons in vivo remains to be established. This study aimed to explore whether administering MYR intravenously, in acute doses, to rats, diminishes the excitability of SpVc wide-dynamic range (WDR) spinal trigeminal nucleus caudalis (SpVc) neurons in response to nociceptive and non-nociceptive mechanical stimulation in vivo. Recordings of extracellular single units were obtained from SpVc neurons when orofacial mechanical stimulation was applied to anesthetized rats. The average firing rate of SpVc WDR neurons, to both non-noxious and noxious mechanical stimuli, was significantly and dose-dependently inhibited by MYR (1–5 mM, intravenously), and the maximum reversible inhibition of the discharge frequency, for both non-noxious and noxious mechanical stimuli, occurred within 5–10 min. The suppressive effects of MYR continued for about 20 min. These findings indicate that an acute, intravenous administration of MYR reduces the SpVc nociceptive transmission, likely through the inhibition of the CaV channels and by activating the Kv channels. Therefore, MYR might be utilized as a treatment for trigeminal nociceptive pain, without causing side effects.https://www.mdpi.com/1420-3049/30/5/1019nociceptionmyricetintrigeminal spinal nucleus caudalissingle-unit recordingCaV channelKv channel |
| spellingShingle | Sana Yamaguchi Risako Chida Syogo Utugi Yukito Sashide Mamoru Takeda Systemic Administration of the Phytochemical, Myricetin, Attenuates the Excitability of Rat Nociceptive Secondary Trigeminal Neurons Molecules nociception myricetin trigeminal spinal nucleus caudalis single-unit recording CaV channel Kv channel |
| title | Systemic Administration of the Phytochemical, Myricetin, Attenuates the Excitability of Rat Nociceptive Secondary Trigeminal Neurons |
| title_full | Systemic Administration of the Phytochemical, Myricetin, Attenuates the Excitability of Rat Nociceptive Secondary Trigeminal Neurons |
| title_fullStr | Systemic Administration of the Phytochemical, Myricetin, Attenuates the Excitability of Rat Nociceptive Secondary Trigeminal Neurons |
| title_full_unstemmed | Systemic Administration of the Phytochemical, Myricetin, Attenuates the Excitability of Rat Nociceptive Secondary Trigeminal Neurons |
| title_short | Systemic Administration of the Phytochemical, Myricetin, Attenuates the Excitability of Rat Nociceptive Secondary Trigeminal Neurons |
| title_sort | systemic administration of the phytochemical myricetin attenuates the excitability of rat nociceptive secondary trigeminal neurons |
| topic | nociception myricetin trigeminal spinal nucleus caudalis single-unit recording CaV channel Kv channel |
| url | https://www.mdpi.com/1420-3049/30/5/1019 |
| work_keys_str_mv | AT sanayamaguchi systemicadministrationofthephytochemicalmyricetinattenuatestheexcitabilityofratnociceptivesecondarytrigeminalneurons AT risakochida systemicadministrationofthephytochemicalmyricetinattenuatestheexcitabilityofratnociceptivesecondarytrigeminalneurons AT syogoutugi systemicadministrationofthephytochemicalmyricetinattenuatestheexcitabilityofratnociceptivesecondarytrigeminalneurons AT yukitosashide systemicadministrationofthephytochemicalmyricetinattenuatestheexcitabilityofratnociceptivesecondarytrigeminalneurons AT mamorutakeda systemicadministrationofthephytochemicalmyricetinattenuatestheexcitabilityofratnociceptivesecondarytrigeminalneurons |