Gip1 GPCR regulates two sexual-stage differentiation processes in the ascomycete Fusarium graminearum

Abstract In ascomycetes, perithecium development involves sexual differentiation processes regulated by mating-related signaling pathways and mating-type locus (MAT) transcription factors, activated by uncharacterized receptors in response to stage-specific signaling cues. Here, we show that a non-p...

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Main Authors: Mingyu Ding, Wanshan Wang, Yuhua Wang, Panpan Huang, Aliang Xia, Daiying Xu, Huiquan Liu, Haitao Cui, Guanghui Wang, Jin-Rong Xu, Cong Jiang
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-61704-2
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author Mingyu Ding
Wanshan Wang
Yuhua Wang
Panpan Huang
Aliang Xia
Daiying Xu
Huiquan Liu
Haitao Cui
Guanghui Wang
Jin-Rong Xu
Cong Jiang
author_facet Mingyu Ding
Wanshan Wang
Yuhua Wang
Panpan Huang
Aliang Xia
Daiying Xu
Huiquan Liu
Haitao Cui
Guanghui Wang
Jin-Rong Xu
Cong Jiang
author_sort Mingyu Ding
collection DOAJ
description Abstract In ascomycetes, perithecium development involves sexual differentiation processes regulated by mating-related signaling pathways and mating-type locus (MAT) transcription factors, activated by uncharacterized receptors in response to stage-specific signaling cues. Here, we show that a non-pheromone receptor, Gip1, regulates two distinct sexual differentiation processes during perithecial development in the wheat scab fungus Fusarium graminearum. Gip1 controls the formation of perithecium initials via the cAMP-PKA pathway, and regulates subsequent development, including the differentiation of peridia and ascogenous tissues, via the Gpmk1 MAPK pathway. The C-terminal tail of Gip1 is important for intracellular signaling, while its N-terminal region and extracellular loop 3 are key for ligand recognition. Interestingly, all sexual-specific spontaneous suppressors of gip1 had mutations in the FgVeA gene, encoding a component of the Velvet complex, which regulates sexual reproduction in filamentous ascomycetes. These mutations partially rescue defects in either perithecium initiation or maturation in gip1 mutants, and restore upregulation of genes important for perithecium development such as MAT1-1-2 (encoding a MAT transcription factor). Thus, Gip1 controls two early stages of sexual differentiation by activating downstream cAMP signaling and Gpmk1 pathways, which may coordinately regulate the expression of genes important for initial perithecium development via FgVeA.
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spelling doaj-art-7242c794d20d44eaafe771fe651bbb1f2025-08-20T03:43:11ZengNature PortfolioNature Communications2041-17232025-07-0116111610.1038/s41467-025-61704-2Gip1 GPCR regulates two sexual-stage differentiation processes in the ascomycete Fusarium graminearumMingyu Ding0Wanshan Wang1Yuhua Wang2Panpan Huang3Aliang Xia4Daiying Xu5Huiquan Liu6Haitao Cui7Guanghui Wang8Jin-Rong Xu9Cong Jiang10State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F UniversityState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F UniversityState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F UniversityState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F UniversityState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F UniversityState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F UniversityState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F UniversityState Key Laboratory of Wheat Improvement, College of Plant Protection, Shandong Agricultural UniversityState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F UniversityDepartment of Botany and Plant Pathology, Purdue UniversityState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F UniversityAbstract In ascomycetes, perithecium development involves sexual differentiation processes regulated by mating-related signaling pathways and mating-type locus (MAT) transcription factors, activated by uncharacterized receptors in response to stage-specific signaling cues. Here, we show that a non-pheromone receptor, Gip1, regulates two distinct sexual differentiation processes during perithecial development in the wheat scab fungus Fusarium graminearum. Gip1 controls the formation of perithecium initials via the cAMP-PKA pathway, and regulates subsequent development, including the differentiation of peridia and ascogenous tissues, via the Gpmk1 MAPK pathway. The C-terminal tail of Gip1 is important for intracellular signaling, while its N-terminal region and extracellular loop 3 are key for ligand recognition. Interestingly, all sexual-specific spontaneous suppressors of gip1 had mutations in the FgVeA gene, encoding a component of the Velvet complex, which regulates sexual reproduction in filamentous ascomycetes. These mutations partially rescue defects in either perithecium initiation or maturation in gip1 mutants, and restore upregulation of genes important for perithecium development such as MAT1-1-2 (encoding a MAT transcription factor). Thus, Gip1 controls two early stages of sexual differentiation by activating downstream cAMP signaling and Gpmk1 pathways, which may coordinately regulate the expression of genes important for initial perithecium development via FgVeA.https://doi.org/10.1038/s41467-025-61704-2
spellingShingle Mingyu Ding
Wanshan Wang
Yuhua Wang
Panpan Huang
Aliang Xia
Daiying Xu
Huiquan Liu
Haitao Cui
Guanghui Wang
Jin-Rong Xu
Cong Jiang
Gip1 GPCR regulates two sexual-stage differentiation processes in the ascomycete Fusarium graminearum
Nature Communications
title Gip1 GPCR regulates two sexual-stage differentiation processes in the ascomycete Fusarium graminearum
title_full Gip1 GPCR regulates two sexual-stage differentiation processes in the ascomycete Fusarium graminearum
title_fullStr Gip1 GPCR regulates two sexual-stage differentiation processes in the ascomycete Fusarium graminearum
title_full_unstemmed Gip1 GPCR regulates two sexual-stage differentiation processes in the ascomycete Fusarium graminearum
title_short Gip1 GPCR regulates two sexual-stage differentiation processes in the ascomycete Fusarium graminearum
title_sort gip1 gpcr regulates two sexual stage differentiation processes in the ascomycete fusarium graminearum
url https://doi.org/10.1038/s41467-025-61704-2
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