Serum Amyloid A Promotes E-Selectin Expression via Toll-Like Receptor 2 in Human Aortic Endothelial Cells
Periodontitis is a chronic inflammatory disease that affects the periodontium. Recent studies suggest an association between periodontal and cardiovascular diseases. However, the detailed molecular mechanism is unknown. A previous study has demonstrated that experimental periodontitis induces serum...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/7150509 |
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| author | Eisaku Nishida Makoto Aino Shu-ichiro Kobayashi Kosuke Okada Tasuku Ohno Takeshi Kikuchi Jun-ichiro Hayashi Genta Yamamoto Yoshiaki Hasegawa Akio Mitani |
| author_facet | Eisaku Nishida Makoto Aino Shu-ichiro Kobayashi Kosuke Okada Tasuku Ohno Takeshi Kikuchi Jun-ichiro Hayashi Genta Yamamoto Yoshiaki Hasegawa Akio Mitani |
| author_sort | Eisaku Nishida |
| collection | DOAJ |
| description | Periodontitis is a chronic inflammatory disease that affects the periodontium. Recent studies suggest an association between periodontal and cardiovascular diseases. However, the detailed molecular mechanism is unknown. A previous study has demonstrated that experimental periodontitis induces serum amyloid A (SAA) in the liver and peripheral blood of ApoE-deficient mice as an atherosclerosis model. SAA is an acute-phase protein that affects systemic inflammation. The aim of this study is to investigate the atherosclerosis-onset mechanism using human aortic endothelial cells (HAECs) stimulated by SAA in vitro. Atherosclerosis PCR array and qPCR analyses showed upregulation of adhesion molecules such as intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and E-selectin in HAECs upon SAA stimulation. In addition, the results demonstrated that Toll-like receptor, TLR2, could serve as an important receptor of SAA in HAECs. Furthermore, small interfering RNA (siRNA) against TLR2 inhibited the upregulation of adhesion molecules in HAECs stimulated by SAA. Our results suggest that SAA stimulates the expression of adhesion molecules via TLR2. SAA could be an important molecule for atherosclerosis induced by periodontal disease. |
| format | Article |
| id | doaj-art-72382f3ff7574e79aeb6cb8c4a974adc |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-72382f3ff7574e79aeb6cb8c4a974adc2025-08-20T02:06:27ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/71505097150509Serum Amyloid A Promotes E-Selectin Expression via Toll-Like Receptor 2 in Human Aortic Endothelial CellsEisaku Nishida0Makoto Aino1Shu-ichiro Kobayashi2Kosuke Okada3Tasuku Ohno4Takeshi Kikuchi5Jun-ichiro Hayashi6Genta Yamamoto7Yoshiaki Hasegawa8Akio Mitani9Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, JapanDepartment of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, JapanDepartment of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, JapanDepartment of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, JapanDepartment of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, JapanDepartment of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, JapanDepartment of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, JapanDepartment of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, JapanDepartment of Microbiology, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, JapanDepartment of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, JapanPeriodontitis is a chronic inflammatory disease that affects the periodontium. Recent studies suggest an association between periodontal and cardiovascular diseases. However, the detailed molecular mechanism is unknown. A previous study has demonstrated that experimental periodontitis induces serum amyloid A (SAA) in the liver and peripheral blood of ApoE-deficient mice as an atherosclerosis model. SAA is an acute-phase protein that affects systemic inflammation. The aim of this study is to investigate the atherosclerosis-onset mechanism using human aortic endothelial cells (HAECs) stimulated by SAA in vitro. Atherosclerosis PCR array and qPCR analyses showed upregulation of adhesion molecules such as intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and E-selectin in HAECs upon SAA stimulation. In addition, the results demonstrated that Toll-like receptor, TLR2, could serve as an important receptor of SAA in HAECs. Furthermore, small interfering RNA (siRNA) against TLR2 inhibited the upregulation of adhesion molecules in HAECs stimulated by SAA. Our results suggest that SAA stimulates the expression of adhesion molecules via TLR2. SAA could be an important molecule for atherosclerosis induced by periodontal disease.http://dx.doi.org/10.1155/2016/7150509 |
| spellingShingle | Eisaku Nishida Makoto Aino Shu-ichiro Kobayashi Kosuke Okada Tasuku Ohno Takeshi Kikuchi Jun-ichiro Hayashi Genta Yamamoto Yoshiaki Hasegawa Akio Mitani Serum Amyloid A Promotes E-Selectin Expression via Toll-Like Receptor 2 in Human Aortic Endothelial Cells Mediators of Inflammation |
| title | Serum Amyloid A Promotes E-Selectin Expression via Toll-Like Receptor 2 in Human Aortic Endothelial Cells |
| title_full | Serum Amyloid A Promotes E-Selectin Expression via Toll-Like Receptor 2 in Human Aortic Endothelial Cells |
| title_fullStr | Serum Amyloid A Promotes E-Selectin Expression via Toll-Like Receptor 2 in Human Aortic Endothelial Cells |
| title_full_unstemmed | Serum Amyloid A Promotes E-Selectin Expression via Toll-Like Receptor 2 in Human Aortic Endothelial Cells |
| title_short | Serum Amyloid A Promotes E-Selectin Expression via Toll-Like Receptor 2 in Human Aortic Endothelial Cells |
| title_sort | serum amyloid a promotes e selectin expression via toll like receptor 2 in human aortic endothelial cells |
| url | http://dx.doi.org/10.1155/2016/7150509 |
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