Whole genome sequencing of Salmonella in poultry from China reveals the presence of blaNDM-5 in different serotypes
Salmonella is a significant zoonotic pathogen, which carries diverse antibiotic resistance genes (ARGs) and utilizes poultry as a major reservoir. It spreads along the farm-to-table continuum, posing risks of human salmonellosis and clinical infections. In this study, 144 Salmonella strains were iso...
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Elsevier
2025-11-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125008880 |
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| author | Yi Zhou Ke Wu Heng Lin Yu-Lian Hu Wei Xu Jie Zhang Sheng Zhou Kai Yu Chun-Guo Liu Hong-Ning Wang Chang-Wei Lei |
| author_facet | Yi Zhou Ke Wu Heng Lin Yu-Lian Hu Wei Xu Jie Zhang Sheng Zhou Kai Yu Chun-Guo Liu Hong-Ning Wang Chang-Wei Lei |
| author_sort | Yi Zhou |
| collection | DOAJ |
| description | Salmonella is a significant zoonotic pathogen, which carries diverse antibiotic resistance genes (ARGs) and utilizes poultry as a major reservoir. It spreads along the farm-to-table continuum, posing risks of human salmonellosis and clinical infections. In this study, 144 Salmonella strains were isolated from 741 poultry samples across 23 provinces in China. Whole genome sequencing (WGS) identified 16 serotypes and 18 sequence types (STs); the main serotypes are S. Enteritidis, S. Kentucky, S. Indiana and S. Typhimurium. SNP analysis of S. Enteritidis and S. Kentucky revealed potential transmission events (SNPs < 10), which were concentrated in close provinces. Antimicrobial susceptibility testing provides information on antimicrobial resistance phenotype, with over 50 % exhibiting multidrug resistance (MDR). Bioinformatics analysis revealed 67 ARGs in all strains, including 6 blaNDM-5-positive isolates. Nanopore sequencing revealed that blaNDM-5 genes in different isolates were located on IncHI2/ST3 plasmids or chromosomes. The ability of horizontal transfer was verified by inverse PCR and conjugation experiments. Notably, this is the first report of blaNDM-5-positive S. Idikan. This study revealed Salmonella's current prevalence status in poultry from China and emphasized the transmission of blaNDM-5 in poultry, which can help guide the rational use of antibiotics and the formulation of relevant rules in poultry breeding. |
| format | Article |
| id | doaj-art-72346cf5e1ca45d6aa0a3726d20080ea |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-72346cf5e1ca45d6aa0a3726d20080ea2025-08-20T03:41:57ZengElsevierPoultry Science0032-57912025-11-011041110564710.1016/j.psj.2025.105647Whole genome sequencing of Salmonella in poultry from China reveals the presence of blaNDM-5 in different serotypesYi Zhou0Ke Wu1Heng Lin2Yu-Lian Hu3Wei Xu4Jie Zhang5Sheng Zhou6Kai Yu7Chun-Guo Liu8Hong-Ning Wang9Chang-Wei Lei10Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, NO. 29 Wangjiang Road, Chengdu, Sichuan 610064, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, NO. 29 Wangjiang Road, Chengdu, Sichuan 610064, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, NO. 29 Wangjiang Road, Chengdu, Sichuan 610064, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, NO. 29 Wangjiang Road, Chengdu, Sichuan 610064, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, NO. 29 Wangjiang Road, Chengdu, Sichuan 610064, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, NO. 29 Wangjiang Road, Chengdu, Sichuan 610064, ChinaShandong Sinder Technology Co., Ltd., Qingdao, ChinaShandong Sinder Technology Co., Ltd., Qingdao, ChinaShandong Sinder Technology Co., Ltd., Qingdao, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, NO. 29 Wangjiang Road, Chengdu, Sichuan 610064, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, NO. 29 Wangjiang Road, Chengdu, Sichuan 610064, China; Corresponding author.Salmonella is a significant zoonotic pathogen, which carries diverse antibiotic resistance genes (ARGs) and utilizes poultry as a major reservoir. It spreads along the farm-to-table continuum, posing risks of human salmonellosis and clinical infections. In this study, 144 Salmonella strains were isolated from 741 poultry samples across 23 provinces in China. Whole genome sequencing (WGS) identified 16 serotypes and 18 sequence types (STs); the main serotypes are S. Enteritidis, S. Kentucky, S. Indiana and S. Typhimurium. SNP analysis of S. Enteritidis and S. Kentucky revealed potential transmission events (SNPs < 10), which were concentrated in close provinces. Antimicrobial susceptibility testing provides information on antimicrobial resistance phenotype, with over 50 % exhibiting multidrug resistance (MDR). Bioinformatics analysis revealed 67 ARGs in all strains, including 6 blaNDM-5-positive isolates. Nanopore sequencing revealed that blaNDM-5 genes in different isolates were located on IncHI2/ST3 plasmids or chromosomes. The ability of horizontal transfer was verified by inverse PCR and conjugation experiments. Notably, this is the first report of blaNDM-5-positive S. Idikan. This study revealed Salmonella's current prevalence status in poultry from China and emphasized the transmission of blaNDM-5 in poultry, which can help guide the rational use of antibiotics and the formulation of relevant rules in poultry breeding.http://www.sciencedirect.com/science/article/pii/S0032579125008880Salmonella entericaPoultryWhole genome sequencingblaNDM-5 |
| spellingShingle | Yi Zhou Ke Wu Heng Lin Yu-Lian Hu Wei Xu Jie Zhang Sheng Zhou Kai Yu Chun-Guo Liu Hong-Ning Wang Chang-Wei Lei Whole genome sequencing of Salmonella in poultry from China reveals the presence of blaNDM-5 in different serotypes Poultry Science Salmonella enterica Poultry Whole genome sequencing blaNDM-5 |
| title | Whole genome sequencing of Salmonella in poultry from China reveals the presence of blaNDM-5 in different serotypes |
| title_full | Whole genome sequencing of Salmonella in poultry from China reveals the presence of blaNDM-5 in different serotypes |
| title_fullStr | Whole genome sequencing of Salmonella in poultry from China reveals the presence of blaNDM-5 in different serotypes |
| title_full_unstemmed | Whole genome sequencing of Salmonella in poultry from China reveals the presence of blaNDM-5 in different serotypes |
| title_short | Whole genome sequencing of Salmonella in poultry from China reveals the presence of blaNDM-5 in different serotypes |
| title_sort | whole genome sequencing of salmonella in poultry from china reveals the presence of blandm 5 in different serotypes |
| topic | Salmonella enterica Poultry Whole genome sequencing blaNDM-5 |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125008880 |
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