A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents
To bridge the convergence between traditional Chinese medicine (TCM) and modern medicine originated from the West, a new method of area under the absorbance-wavelength curve (AUAWC) by spectrophotometer scanning was investigated and compared with HPLC method to explore metabolomic pharmacokinetics i...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2013/919023 |
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| author | Lihong Zhang Xiaojin Xiao Zhenzhen Yang Mengli Jiang Xiaodong Li |
| author_facet | Lihong Zhang Xiaojin Xiao Zhenzhen Yang Mengli Jiang Xiaodong Li |
| author_sort | Lihong Zhang |
| collection | DOAJ |
| description | To bridge the convergence between traditional Chinese medicine (TCM) and modern medicine originated from the West, a new method of area under the absorbance-wavelength curve (AUAWC) by spectrophotometer scanning was investigated and compared with HPLC method to explore metabolomic pharmacokinetics in rats. AUAWC and drug total concentration were obtained after Yangxue was injected to rats. Meanwhile, individual concentrations of sodium ferulate, tetramethylpyrazine hydrochloride, tanshinol sodium, and sodium tanshinone IIA sulfonate in plasma were determined by HPLC. Metabolomic profile of multicomponents plasma concentration time from AUAWC and that of individual components from HPLC were compared. The data from AUAWC had one-compartment model with mean area under concentration versus time (AUC) of 9370.58 min·μg/mL and mean elimination half-life (t1/2) of 12.92 min. The results by HPLC demonstrated that sodium ferulate and tetramethylpyrazine hydrochloride had one-compartment model with AUC of 6075.50 and 876.94 min·μg/mL, t1/2 of 10.85 and 20.57 min, respectively. Tanshinol sodium and sodium tanshinone IIA sulfonate showed two-compartment model, and AUC was 29.58 and 201.46 with t1/2β of 1.76 and 16.90, respectively. The profiles indicated that method of AUAWC can be used to study pharmacokinetics of TCM with multicomponents and to improve its development of active theory and application in clinic combined with in vivo metabolomic profile of HPLC. |
| format | Article |
| id | doaj-art-7222ab213a024d9fb4ce693a2287cdf1 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-7222ab213a024d9fb4ce693a2287cdf12025-08-20T03:33:43ZengWileyJournal of Spectroscopy2314-49202314-49392013-01-01201310.1155/2013/919023919023A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with MulticomponentsLihong Zhang0Xiaojin Xiao1Zhenzhen Yang2Mengli Jiang3Xiaodong Li4College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian, Fuzhou 350122, ChinaCollege of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian, Fuzhou 350122, ChinaCollege of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian, Fuzhou 350122, ChinaCollege of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian, Fuzhou 350122, ChinaCollege of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian, Fuzhou 350122, ChinaTo bridge the convergence between traditional Chinese medicine (TCM) and modern medicine originated from the West, a new method of area under the absorbance-wavelength curve (AUAWC) by spectrophotometer scanning was investigated and compared with HPLC method to explore metabolomic pharmacokinetics in rats. AUAWC and drug total concentration were obtained after Yangxue was injected to rats. Meanwhile, individual concentrations of sodium ferulate, tetramethylpyrazine hydrochloride, tanshinol sodium, and sodium tanshinone IIA sulfonate in plasma were determined by HPLC. Metabolomic profile of multicomponents plasma concentration time from AUAWC and that of individual components from HPLC were compared. The data from AUAWC had one-compartment model with mean area under concentration versus time (AUC) of 9370.58 min·μg/mL and mean elimination half-life (t1/2) of 12.92 min. The results by HPLC demonstrated that sodium ferulate and tetramethylpyrazine hydrochloride had one-compartment model with AUC of 6075.50 and 876.94 min·μg/mL, t1/2 of 10.85 and 20.57 min, respectively. Tanshinol sodium and sodium tanshinone IIA sulfonate showed two-compartment model, and AUC was 29.58 and 201.46 with t1/2β of 1.76 and 16.90, respectively. The profiles indicated that method of AUAWC can be used to study pharmacokinetics of TCM with multicomponents and to improve its development of active theory and application in clinic combined with in vivo metabolomic profile of HPLC.http://dx.doi.org/10.1155/2013/919023 |
| spellingShingle | Lihong Zhang Xiaojin Xiao Zhenzhen Yang Mengli Jiang Xiaodong Li A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents Journal of Spectroscopy |
| title | A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents |
| title_full | A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents |
| title_fullStr | A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents |
| title_full_unstemmed | A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents |
| title_short | A New Method of Area under the Absorbance-Wavelength Curve for Rats Total Metabolomic Pharmacokinetics from Yangxue Injection with Multicomponents |
| title_sort | new method of area under the absorbance wavelength curve for rats total metabolomic pharmacokinetics from yangxue injection with multicomponents |
| url | http://dx.doi.org/10.1155/2013/919023 |
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