Bioaffinity-based method for catecholamines determination with amperometric DNA sensor
A bioaffinity-based method for the determination of adrenaline and dopamine involving immobilized renatured DNA incorporated into the amperometric biosensor was developed. With the help of spectrophometry and voltammetry data, it was found that the composition of complexes formed with catecholamine...
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| Format: | Article |
| Language: | English |
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Kazan Federal University
2020-03-01
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| Series: | Учёные записки Казанского университета: Серия Естественные науки |
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| Online Access: | https://kpfu.ru/uz-eng-ns-2020-1-1.html |
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| author | S.S. Babkina N.A. Ulakhovich E.P. Medyantseva A.R. Gataulina |
| author_facet | S.S. Babkina N.A. Ulakhovich E.P. Medyantseva A.R. Gataulina |
| author_sort | S.S. Babkina |
| collection | DOAJ |
| description | A bioaffinity-based method for the determination of adrenaline and dopamine involving immobilized renatured DNA incorporated into the amperometric biosensor was developed.
With the help of spectrophometry and voltammetry data, it was found that the composition of complexes formed with catecholamines and their stability constants were changing with concentration from [КАL2] to [КА2L], where КА – catecholamine, L – base pare, and lg βeff = 10.1–13.5. The high affinity of adrenaline and dopamine to the immobilized renatured DNA molecule was revealed, thereby making it possible to run effective concentration of KA from the analyzed solutions of low concentration including multicomponent fluids.
The minimum reporting level was found to be 5.5·10–9 (adrenaline) and 2.0·10–8 (dopamin) mol/L, respectively. The proposed method is characterized by high sensitivity, reproducibility (relative accuracy does not exceed 5%), and single determination time shorter than 30 min. |
| format | Article |
| id | doaj-art-72196f522ce04e03b6d433d255dc7ed2 |
| institution | Kabale University |
| issn | 2542-064X 2500-218X |
| language | English |
| publishDate | 2020-03-01 |
| publisher | Kazan Federal University |
| record_format | Article |
| series | Учёные записки Казанского университета: Серия Естественные науки |
| spelling | doaj-art-72196f522ce04e03b6d433d255dc7ed22025-01-03T00:32:59ZengKazan Federal UniversityУчёные записки Казанского университета: Серия Естественные науки2542-064X2500-218X2020-03-01162151610.26907/2542-064X.2020.1.5-16Bioaffinity-based method for catecholamines determination with amperometric DNA sensorS.S. Babkina0N.A. Ulakhovich1E.P. Medyantseva2A.R. Gataulina3MIREA – Russian Technological University, Moscow, 119454 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaA bioaffinity-based method for the determination of adrenaline and dopamine involving immobilized renatured DNA incorporated into the amperometric biosensor was developed. With the help of spectrophometry and voltammetry data, it was found that the composition of complexes formed with catecholamines and their stability constants were changing with concentration from [КАL2] to [КА2L], where КА – catecholamine, L – base pare, and lg βeff = 10.1–13.5. The high affinity of adrenaline and dopamine to the immobilized renatured DNA molecule was revealed, thereby making it possible to run effective concentration of KA from the analyzed solutions of low concentration including multicomponent fluids. The minimum reporting level was found to be 5.5·10–9 (adrenaline) and 2.0·10–8 (dopamin) mol/L, respectively. The proposed method is characterized by high sensitivity, reproducibility (relative accuracy does not exceed 5%), and single determination time shorter than 30 min.https://kpfu.ru/uz-eng-ns-2020-1-1.htmladrenalineamperometric sensorbioaffinity-based methodbiosensordnadopaminecatecholamines |
| spellingShingle | S.S. Babkina N.A. Ulakhovich E.P. Medyantseva A.R. Gataulina Bioaffinity-based method for catecholamines determination with amperometric DNA sensor Учёные записки Казанского университета: Серия Естественные науки adrenaline amperometric sensor bioaffinity-based method biosensor dna dopamine catecholamines |
| title | Bioaffinity-based method for catecholamines determination with amperometric DNA sensor |
| title_full | Bioaffinity-based method for catecholamines determination with amperometric DNA sensor |
| title_fullStr | Bioaffinity-based method for catecholamines determination with amperometric DNA sensor |
| title_full_unstemmed | Bioaffinity-based method for catecholamines determination with amperometric DNA sensor |
| title_short | Bioaffinity-based method for catecholamines determination with amperometric DNA sensor |
| title_sort | bioaffinity based method for catecholamines determination with amperometric dna sensor |
| topic | adrenaline amperometric sensor bioaffinity-based method biosensor dna dopamine catecholamines |
| url | https://kpfu.ru/uz-eng-ns-2020-1-1.html |
| work_keys_str_mv | AT ssbabkina bioaffinitybasedmethodforcatecholaminesdeterminationwithamperometricdnasensor AT naulakhovich bioaffinitybasedmethodforcatecholaminesdeterminationwithamperometricdnasensor AT epmedyantseva bioaffinitybasedmethodforcatecholaminesdeterminationwithamperometricdnasensor AT argataulina bioaffinitybasedmethodforcatecholaminesdeterminationwithamperometricdnasensor |