Gastroprotective properties of flavonoid-rich extract of Pulicaria odora against ethanol-induced gastric ulcer in mice
Pulicaria odora has been traditionally used in northeastern Algeria for treating gastrointestinal disorders, particularly ulcers. This study aimed to assess the gastroprotective, anti-inflammatory, and antioxidant properties of a crude hydroalcoholic extract derived from the leaves of Pulicaria odor...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025000040 |
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| author | Khadidja Boudebbaz Manel Brouk Roumaissa Laalem Nada Zabaiou |
| author_facet | Khadidja Boudebbaz Manel Brouk Roumaissa Laalem Nada Zabaiou |
| author_sort | Khadidja Boudebbaz |
| collection | DOAJ |
| description | Pulicaria odora has been traditionally used in northeastern Algeria for treating gastrointestinal disorders, particularly ulcers. This study aimed to assess the gastroprotective, anti-inflammatory, and antioxidant properties of a crude hydroalcoholic extract derived from the leaves of Pulicaria odora, as well as its subsequent fractions. The gastroprotective effect was studied in an ethanol-induced ulcer model in mice. The in vitro antioxidant activity was quantified via the DPPH, and ABTS methods. In vitro anti-inflammatory activity was investigated through the human red blood cell membrane stabilization method and the bovine serum albumin denaturation method. The in vivo anti-inflammatory properties of the crude extract were assessed in mice via a carrageenan-induced acute paw edema model. The crude extract significantly inhibited gastric ulcer formation in a dose-dependent manner, achieving a reduction of up to 99.91 % at a dose of 200 mg/kg. Among the fractions, the aqueous fraction exhibited the most potent gastroprotective effect, with a notable dose-dependent response and high ulcer inhibition rates (95.56 % at 50 mg/kg). Additionally, the crude extract displayed strong in vitro and in vivo anti-inflammatory activity and a high free radical scavenging capacity; with IC50 values comparable to those of ascorbic acid. LC-ESI-MSn analysis led to the identification of nine flavonoid derivatives, which were subsequently subjected to molecular docking techniques to predict their antiulcer activity. These results suggest that both glycosylated and aglycone flavonoids derived from P. odora have the potential to inhibit gastric acid secretion by targeting the proton pump. |
| format | Article |
| id | doaj-art-72141058214a4384ab882358c873ca69 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj-art-72141058214a4384ab882358c873ca692025-01-17T04:51:46ZengElsevierHeliyon2405-84402025-01-01111e41625Gastroprotective properties of flavonoid-rich extract of Pulicaria odora against ethanol-induced gastric ulcer in miceKhadidja Boudebbaz0Manel Brouk1Roumaissa Laalem2Nada Zabaiou3Laboratory of Pharmacology and Phytochemistry, Faculty of Exact Sciences and Informatics, University of Jijel, 18000, Jijel, Algeria; Corresponding author.Department of Chemistry, Faculty of Exact Sciences and Informatics, University of Jijel, 18000, Jijel, AlgeriaDepartment of Chemistry, Faculty of Exact Sciences and Informatics, University of Jijel, 18000, Jijel, AlgeriaLaboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Jijel, 18000, Jijel, AlgeriaPulicaria odora has been traditionally used in northeastern Algeria for treating gastrointestinal disorders, particularly ulcers. This study aimed to assess the gastroprotective, anti-inflammatory, and antioxidant properties of a crude hydroalcoholic extract derived from the leaves of Pulicaria odora, as well as its subsequent fractions. The gastroprotective effect was studied in an ethanol-induced ulcer model in mice. The in vitro antioxidant activity was quantified via the DPPH, and ABTS methods. In vitro anti-inflammatory activity was investigated through the human red blood cell membrane stabilization method and the bovine serum albumin denaturation method. The in vivo anti-inflammatory properties of the crude extract were assessed in mice via a carrageenan-induced acute paw edema model. The crude extract significantly inhibited gastric ulcer formation in a dose-dependent manner, achieving a reduction of up to 99.91 % at a dose of 200 mg/kg. Among the fractions, the aqueous fraction exhibited the most potent gastroprotective effect, with a notable dose-dependent response and high ulcer inhibition rates (95.56 % at 50 mg/kg). Additionally, the crude extract displayed strong in vitro and in vivo anti-inflammatory activity and a high free radical scavenging capacity; with IC50 values comparable to those of ascorbic acid. LC-ESI-MSn analysis led to the identification of nine flavonoid derivatives, which were subsequently subjected to molecular docking techniques to predict their antiulcer activity. These results suggest that both glycosylated and aglycone flavonoids derived from P. odora have the potential to inhibit gastric acid secretion by targeting the proton pump.http://www.sciencedirect.com/science/article/pii/S2405844025000040Pulicaria odoraGastroprotective effectsAnti-inflammatory propertiesAntioxidant activityFlavonoidsMolecular docking |
| spellingShingle | Khadidja Boudebbaz Manel Brouk Roumaissa Laalem Nada Zabaiou Gastroprotective properties of flavonoid-rich extract of Pulicaria odora against ethanol-induced gastric ulcer in mice Heliyon Pulicaria odora Gastroprotective effects Anti-inflammatory properties Antioxidant activity Flavonoids Molecular docking |
| title | Gastroprotective properties of flavonoid-rich extract of Pulicaria odora against ethanol-induced gastric ulcer in mice |
| title_full | Gastroprotective properties of flavonoid-rich extract of Pulicaria odora against ethanol-induced gastric ulcer in mice |
| title_fullStr | Gastroprotective properties of flavonoid-rich extract of Pulicaria odora against ethanol-induced gastric ulcer in mice |
| title_full_unstemmed | Gastroprotective properties of flavonoid-rich extract of Pulicaria odora against ethanol-induced gastric ulcer in mice |
| title_short | Gastroprotective properties of flavonoid-rich extract of Pulicaria odora against ethanol-induced gastric ulcer in mice |
| title_sort | gastroprotective properties of flavonoid rich extract of pulicaria odora against ethanol induced gastric ulcer in mice |
| topic | Pulicaria odora Gastroprotective effects Anti-inflammatory properties Antioxidant activity Flavonoids Molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025000040 |
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