Unraveling the association and regulatory role of miR-146b-5p in coronary artery disease
Abstract Background Coronary artery disease (CAD), one of the most prevalent cardiovascular diseases, is a critical health issue that affects millions of individuals worldwide. It has been reported that miR-146b-5p exhibited a strong correlation with inflammatory responses and atherosclerosis. Howev...
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BMC
2025-02-01
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| Series: | BMC Cardiovascular Disorders |
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| Online Access: | https://doi.org/10.1186/s12872-025-04530-0 |
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| author | Xiaozhu Ma Shuai Mei Yi He Qidamugai Wuyun Li Zhou Ziyang Cai Qiushi Luo Yi Wen Jiangtao Yan |
| author_facet | Xiaozhu Ma Shuai Mei Yi He Qidamugai Wuyun Li Zhou Ziyang Cai Qiushi Luo Yi Wen Jiangtao Yan |
| author_sort | Xiaozhu Ma |
| collection | DOAJ |
| description | Abstract Background Coronary artery disease (CAD), one of the most prevalent cardiovascular diseases, is a critical health issue that affects millions of individuals worldwide. It has been reported that miR-146b-5p exhibited a strong correlation with inflammatory responses and atherosclerosis. However, its association with the incidence and severity of CAD has not been substantiated in a large cohort. In the study, we focus on the expression of miR-146b-5p in peripheral blood mononuclear cells (PBMCs) of patients with CAD and preliminarily investigate its function and underlying mechanism. Methods and results The study encompassed a total of 452 participants, consisting 295 patients with CAD and 157 individuals without CAD. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) was performed to assess miR-146b-5p expression in PBMCs. We found that miR-146b-5p was significantly increased in PBMCs of patients with CAD compared with the control group. Binary logistic regression revealed that miR-146b-5p was associated with CAD. Receiver Operation Characteristic (ROC) analysis showed that the sensitivity and specificity of miR-146b-5p in discriminating CAD patients from non-CAD patients were meaningful. Subsequent subgroup analysis showed that miR-146b-5p was related to the severity of CAD. Furthermore, gain- and loss-of-function experiments in THP-1 cells showed that miR-146b-5p inhibited inflammation, cell proliferation, and migration. Mechanically, miR-146b-5p was involved in the classical NF-κB inflammatory pathway by directly targeting IKKβ. Conclusion Our study revealed that miR-146b-5p was higher in the PBMCs of CAD patients than non-CAD individuals, and established a correlation between miR-146b-5p and occurrence and severity of CAD. In addition, the inflammatory role of miR-146b-5p is mediated by targeting IKKβ. |
| format | Article |
| id | doaj-art-720ffa23b42142e0b22e1db1a0ff4ce7 |
| institution | Kabale University |
| issn | 1471-2261 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cardiovascular Disorders |
| spelling | doaj-art-720ffa23b42142e0b22e1db1a0ff4ce72025-02-09T12:11:20ZengBMCBMC Cardiovascular Disorders1471-22612025-02-0125111510.1186/s12872-025-04530-0Unraveling the association and regulatory role of miR-146b-5p in coronary artery diseaseXiaozhu Ma0Shuai Mei1Yi He2Qidamugai Wuyun3Li Zhou4Ziyang Cai5Qiushi Luo6Yi Wen7Jiangtao Yan8Department of Cardiology, Division of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & TechnologyDepartment of Cardiology, Division of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & TechnologyDepartment of Cardiology, Division of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & TechnologyDepartment of Cardiology, Division of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & TechnologyDepartment of Cardiology, Division of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & TechnologyDepartment of Cardiology, Division of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & TechnologyDepartment of Cardiology, Division of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & TechnologyDepartment of Cardiology, Division of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & TechnologyDepartment of Cardiology, Division of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science & TechnologyAbstract Background Coronary artery disease (CAD), one of the most prevalent cardiovascular diseases, is a critical health issue that affects millions of individuals worldwide. It has been reported that miR-146b-5p exhibited a strong correlation with inflammatory responses and atherosclerosis. However, its association with the incidence and severity of CAD has not been substantiated in a large cohort. In the study, we focus on the expression of miR-146b-5p in peripheral blood mononuclear cells (PBMCs) of patients with CAD and preliminarily investigate its function and underlying mechanism. Methods and results The study encompassed a total of 452 participants, consisting 295 patients with CAD and 157 individuals without CAD. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) was performed to assess miR-146b-5p expression in PBMCs. We found that miR-146b-5p was significantly increased in PBMCs of patients with CAD compared with the control group. Binary logistic regression revealed that miR-146b-5p was associated with CAD. Receiver Operation Characteristic (ROC) analysis showed that the sensitivity and specificity of miR-146b-5p in discriminating CAD patients from non-CAD patients were meaningful. Subsequent subgroup analysis showed that miR-146b-5p was related to the severity of CAD. Furthermore, gain- and loss-of-function experiments in THP-1 cells showed that miR-146b-5p inhibited inflammation, cell proliferation, and migration. Mechanically, miR-146b-5p was involved in the classical NF-κB inflammatory pathway by directly targeting IKKβ. Conclusion Our study revealed that miR-146b-5p was higher in the PBMCs of CAD patients than non-CAD individuals, and established a correlation between miR-146b-5p and occurrence and severity of CAD. In addition, the inflammatory role of miR-146b-5p is mediated by targeting IKKβ.https://doi.org/10.1186/s12872-025-04530-0miR-146b-5pCoronary artery diseaseInflammationIKKB |
| spellingShingle | Xiaozhu Ma Shuai Mei Yi He Qidamugai Wuyun Li Zhou Ziyang Cai Qiushi Luo Yi Wen Jiangtao Yan Unraveling the association and regulatory role of miR-146b-5p in coronary artery disease BMC Cardiovascular Disorders miR-146b-5p Coronary artery disease Inflammation IKKB |
| title | Unraveling the association and regulatory role of miR-146b-5p in coronary artery disease |
| title_full | Unraveling the association and regulatory role of miR-146b-5p in coronary artery disease |
| title_fullStr | Unraveling the association and regulatory role of miR-146b-5p in coronary artery disease |
| title_full_unstemmed | Unraveling the association and regulatory role of miR-146b-5p in coronary artery disease |
| title_short | Unraveling the association and regulatory role of miR-146b-5p in coronary artery disease |
| title_sort | unraveling the association and regulatory role of mir 146b 5p in coronary artery disease |
| topic | miR-146b-5p Coronary artery disease Inflammation IKKB |
| url | https://doi.org/10.1186/s12872-025-04530-0 |
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