Essential Oil Content of the Rhizome of Curcuma purpurascens Bl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines
Curcuma purpurascens Bl., belonging to the Zingiberaceae family, is known as temu tis in Yogyakarta, Indonesia. In this study, the hydrodistilled dried ground rhizome oil was investigated for its chemical content and antiproliferative activity against selected human carcinoma cell lines (MCF7, Ca Sk...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/397430 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558805190180864 |
|---|---|
| author | Sok-Lai Hong Guan-Serm Lee Syarifah Nur Syed Abdul Rahman Omer Abdalla Ahmed Hamdi Khalijah Awang Nurfina Aznam Nugroho Sri Nurestri Abd Malek |
| author_facet | Sok-Lai Hong Guan-Serm Lee Syarifah Nur Syed Abdul Rahman Omer Abdalla Ahmed Hamdi Khalijah Awang Nurfina Aznam Nugroho Sri Nurestri Abd Malek |
| author_sort | Sok-Lai Hong |
| collection | DOAJ |
| description | Curcuma purpurascens Bl., belonging to the Zingiberaceae family, is known as temu tis in Yogyakarta, Indonesia. In this study, the hydrodistilled dried ground rhizome oil was investigated for its chemical content and antiproliferative activity against selected human carcinoma cell lines (MCF7, Ca Ski, A549, HT29, and HCT116) and a normal human lung fibroblast cell line (MRC5). Results from GC-MS and GC-FID analysis of the rhizome oil of temu tis showed turmerone as the major component, followed by germacrone, ar-turmerone, germacrene-B, and curlone. The rhizome oil of temu tis exhibited strong cytotoxicity against HT29 cells (IC50 value of 4.9 ± 0.4 μg/mL), weak cytotoxicity against A549, Ca Ski, and HCT116 cells (with IC50 values of 46.3 ± 0.7, 32.5 ± 1.1, and 35.0 ± 0.3 μg/mL, resp.), and no inhibitory effect against MCF7 cells. It exhibited mild cytotoxicity against a noncancerous human lung fibroblast cell line (MRC5), with an IC50 value of 25.2 ± 2.7 μg/mL. This is the first report on the chemical composition of this rhizome’s oil and its selective antiproliferative effect on HT29. The obtained data provided a basis for further investigation of the mode of cell death. |
| format | Article |
| id | doaj-art-720ff42dbe3e498da206dd25da3ac495 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-720ff42dbe3e498da206dd25da3ac4952025-02-03T01:31:30ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/397430397430Essential Oil Content of the Rhizome of Curcuma purpurascens Bl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell LinesSok-Lai Hong0Guan-Serm Lee1Syarifah Nur Syed Abdul Rahman2Omer Abdalla Ahmed Hamdi3Khalijah Awang4Nurfina Aznam Nugroho5Sri Nurestri Abd Malek6Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaInstitute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaInstitute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaFaculty of Mathematics and Sciences, Yogyakarta State University, Yogyakarta 55281, IndonesiaInstitute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaCurcuma purpurascens Bl., belonging to the Zingiberaceae family, is known as temu tis in Yogyakarta, Indonesia. In this study, the hydrodistilled dried ground rhizome oil was investigated for its chemical content and antiproliferative activity against selected human carcinoma cell lines (MCF7, Ca Ski, A549, HT29, and HCT116) and a normal human lung fibroblast cell line (MRC5). Results from GC-MS and GC-FID analysis of the rhizome oil of temu tis showed turmerone as the major component, followed by germacrone, ar-turmerone, germacrene-B, and curlone. The rhizome oil of temu tis exhibited strong cytotoxicity against HT29 cells (IC50 value of 4.9 ± 0.4 μg/mL), weak cytotoxicity against A549, Ca Ski, and HCT116 cells (with IC50 values of 46.3 ± 0.7, 32.5 ± 1.1, and 35.0 ± 0.3 μg/mL, resp.), and no inhibitory effect against MCF7 cells. It exhibited mild cytotoxicity against a noncancerous human lung fibroblast cell line (MRC5), with an IC50 value of 25.2 ± 2.7 μg/mL. This is the first report on the chemical composition of this rhizome’s oil and its selective antiproliferative effect on HT29. The obtained data provided a basis for further investigation of the mode of cell death.http://dx.doi.org/10.1155/2014/397430 |
| spellingShingle | Sok-Lai Hong Guan-Serm Lee Syarifah Nur Syed Abdul Rahman Omer Abdalla Ahmed Hamdi Khalijah Awang Nurfina Aznam Nugroho Sri Nurestri Abd Malek Essential Oil Content of the Rhizome of Curcuma purpurascens Bl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines The Scientific World Journal |
| title | Essential Oil Content of the Rhizome of Curcuma purpurascens Bl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines |
| title_full | Essential Oil Content of the Rhizome of Curcuma purpurascens Bl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines |
| title_fullStr | Essential Oil Content of the Rhizome of Curcuma purpurascens Bl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines |
| title_full_unstemmed | Essential Oil Content of the Rhizome of Curcuma purpurascens Bl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines |
| title_short | Essential Oil Content of the Rhizome of Curcuma purpurascens Bl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines |
| title_sort | essential oil content of the rhizome of curcuma purpurascens bl temu tis and its antiproliferative effect on selected human carcinoma cell lines |
| url | http://dx.doi.org/10.1155/2014/397430 |
| work_keys_str_mv | AT soklaihong essentialoilcontentoftherhizomeofcurcumapurpurascensbltemutisanditsantiproliferativeeffectonselectedhumancarcinomacelllines AT guansermlee essentialoilcontentoftherhizomeofcurcumapurpurascensbltemutisanditsantiproliferativeeffectonselectedhumancarcinomacelllines AT syarifahnursyedabdulrahman essentialoilcontentoftherhizomeofcurcumapurpurascensbltemutisanditsantiproliferativeeffectonselectedhumancarcinomacelllines AT omerabdallaahmedhamdi essentialoilcontentoftherhizomeofcurcumapurpurascensbltemutisanditsantiproliferativeeffectonselectedhumancarcinomacelllines AT khalijahawang essentialoilcontentoftherhizomeofcurcumapurpurascensbltemutisanditsantiproliferativeeffectonselectedhumancarcinomacelllines AT nurfinaaznamnugroho essentialoilcontentoftherhizomeofcurcumapurpurascensbltemutisanditsantiproliferativeeffectonselectedhumancarcinomacelllines AT srinurestriabdmalek essentialoilcontentoftherhizomeofcurcumapurpurascensbltemutisanditsantiproliferativeeffectonselectedhumancarcinomacelllines |