Nonlytic cellular release of hepatitis A virus requires dual capsid recruitment of the ESCRT-associated Bro1 domain proteins HD-PTP and ALIX.
Although picornaviruses are conventionally considered 'nonenveloped', members of multiple picornaviral genera are released nonlytically from infected cells in extracellular vesicles. The mechanisms underlying this process are poorly understood. Here, we describe interactions of the hepatit...
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Public Library of Science (PLoS)
2022-08-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1010543&type=printable |
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| author | Takayoshi Shirasaki Hui Feng Helen M E Duyvesteyn William G Fusco Kevin L McKnight Ling Xie Mark Boyce Sathish Kumar Rina Barouch-Bentov Olga González-López Ryan McNamara Li Wang Adriana Hertel-Wulff Xian Chen Shirit Einav Joseph A Duncan Maryna Kapustina Elizabeth E Fry David I Stuart Stanley M Lemon |
| author_facet | Takayoshi Shirasaki Hui Feng Helen M E Duyvesteyn William G Fusco Kevin L McKnight Ling Xie Mark Boyce Sathish Kumar Rina Barouch-Bentov Olga González-López Ryan McNamara Li Wang Adriana Hertel-Wulff Xian Chen Shirit Einav Joseph A Duncan Maryna Kapustina Elizabeth E Fry David I Stuart Stanley M Lemon |
| author_sort | Takayoshi Shirasaki |
| collection | DOAJ |
| description | Although picornaviruses are conventionally considered 'nonenveloped', members of multiple picornaviral genera are released nonlytically from infected cells in extracellular vesicles. The mechanisms underlying this process are poorly understood. Here, we describe interactions of the hepatitis A virus (HAV) capsid with components of host endosomal sorting complexes required for transport (ESCRT) that play an essential role in release. We show release of quasi-enveloped virus (eHAV) in exosome-like vesicles requires a conserved export signal located within the 8 kDa C-terminal VP1 pX extension that functions in a manner analogous to late domains of canonical enveloped viruses. Fusing pX to a self-assembling engineered protein nanocage (EPN-pX) resulted in its ESCRT-dependent release in extracellular vesicles. Mutational analysis identified a 24 amino acid peptide sequence located within the center of pX that was both necessary and sufficient for nanocage release. Deleting a YxxL motif within this sequence ablated eHAV release, resulting in virus accumulating intracellularly. The pX export signal is conserved in non-human hepatoviruses from a wide range of mammalian species, and functional in pX sequences from bat hepatoviruses when fused to the nanocage protein, suggesting these viruses are released as quasi-enveloped virions. Quantitative proteomics identified multiple ESCRT-related proteins associating with EPN-pX, including ALG2-interacting protein X (ALIX), and its paralog, tyrosine-protein phosphatase non-receptor type 23 (HD-PTP), a second Bro1 domain protein linked to sorting of ubiquitylated cargo into multivesicular endosomes. RNAi-mediated depletion of either Bro1 domain protein impeded eHAV release. Super-resolution fluorescence microscopy demonstrated colocalization of viral capsids with endogenous ALIX and HD-PTP. Co-immunoprecipitation assays using biotin-tagged peptides and recombinant proteins revealed pX interacts directly through the export signal with N-terminal Bro1 domains of both HD-PTP and ALIX. Our study identifies an exceptionally potent viral export signal mediating extracellular release of virus-sized protein assemblies and shows release requires non-redundant activities of both HD-PTP and ALIX. |
| format | Article |
| id | doaj-art-71fe0a3da16d43cda03ca15add33c19f |
| institution | OA Journals |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS Pathogens |
| spelling | doaj-art-71fe0a3da16d43cda03ca15add33c19f2025-08-20T02:31:38ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742022-08-01188e101054310.1371/journal.ppat.1010543Nonlytic cellular release of hepatitis A virus requires dual capsid recruitment of the ESCRT-associated Bro1 domain proteins HD-PTP and ALIX.Takayoshi ShirasakiHui FengHelen M E DuyvesteynWilliam G FuscoKevin L McKnightLing XieMark BoyceSathish KumarRina Barouch-BentovOlga González-LópezRyan McNamaraLi WangAdriana Hertel-WulffXian ChenShirit EinavJoseph A DuncanMaryna KapustinaElizabeth E FryDavid I StuartStanley M LemonAlthough picornaviruses are conventionally considered 'nonenveloped', members of multiple picornaviral genera are released nonlytically from infected cells in extracellular vesicles. The mechanisms underlying this process are poorly understood. Here, we describe interactions of the hepatitis A virus (HAV) capsid with components of host endosomal sorting complexes required for transport (ESCRT) that play an essential role in release. We show release of quasi-enveloped virus (eHAV) in exosome-like vesicles requires a conserved export signal located within the 8 kDa C-terminal VP1 pX extension that functions in a manner analogous to late domains of canonical enveloped viruses. Fusing pX to a self-assembling engineered protein nanocage (EPN-pX) resulted in its ESCRT-dependent release in extracellular vesicles. Mutational analysis identified a 24 amino acid peptide sequence located within the center of pX that was both necessary and sufficient for nanocage release. Deleting a YxxL motif within this sequence ablated eHAV release, resulting in virus accumulating intracellularly. The pX export signal is conserved in non-human hepatoviruses from a wide range of mammalian species, and functional in pX sequences from bat hepatoviruses when fused to the nanocage protein, suggesting these viruses are released as quasi-enveloped virions. Quantitative proteomics identified multiple ESCRT-related proteins associating with EPN-pX, including ALG2-interacting protein X (ALIX), and its paralog, tyrosine-protein phosphatase non-receptor type 23 (HD-PTP), a second Bro1 domain protein linked to sorting of ubiquitylated cargo into multivesicular endosomes. RNAi-mediated depletion of either Bro1 domain protein impeded eHAV release. Super-resolution fluorescence microscopy demonstrated colocalization of viral capsids with endogenous ALIX and HD-PTP. Co-immunoprecipitation assays using biotin-tagged peptides and recombinant proteins revealed pX interacts directly through the export signal with N-terminal Bro1 domains of both HD-PTP and ALIX. Our study identifies an exceptionally potent viral export signal mediating extracellular release of virus-sized protein assemblies and shows release requires non-redundant activities of both HD-PTP and ALIX.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1010543&type=printable |
| spellingShingle | Takayoshi Shirasaki Hui Feng Helen M E Duyvesteyn William G Fusco Kevin L McKnight Ling Xie Mark Boyce Sathish Kumar Rina Barouch-Bentov Olga González-López Ryan McNamara Li Wang Adriana Hertel-Wulff Xian Chen Shirit Einav Joseph A Duncan Maryna Kapustina Elizabeth E Fry David I Stuart Stanley M Lemon Nonlytic cellular release of hepatitis A virus requires dual capsid recruitment of the ESCRT-associated Bro1 domain proteins HD-PTP and ALIX. PLoS Pathogens |
| title | Nonlytic cellular release of hepatitis A virus requires dual capsid recruitment of the ESCRT-associated Bro1 domain proteins HD-PTP and ALIX. |
| title_full | Nonlytic cellular release of hepatitis A virus requires dual capsid recruitment of the ESCRT-associated Bro1 domain proteins HD-PTP and ALIX. |
| title_fullStr | Nonlytic cellular release of hepatitis A virus requires dual capsid recruitment of the ESCRT-associated Bro1 domain proteins HD-PTP and ALIX. |
| title_full_unstemmed | Nonlytic cellular release of hepatitis A virus requires dual capsid recruitment of the ESCRT-associated Bro1 domain proteins HD-PTP and ALIX. |
| title_short | Nonlytic cellular release of hepatitis A virus requires dual capsid recruitment of the ESCRT-associated Bro1 domain proteins HD-PTP and ALIX. |
| title_sort | nonlytic cellular release of hepatitis a virus requires dual capsid recruitment of the escrt associated bro1 domain proteins hd ptp and alix |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1010543&type=printable |
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