A case report of hermaphrodite goat: clinical and genetic approach

This case report describes clinical examination and cytogenetic and molecular genetic analyses of a hermaphrodite mixed Maltese breed goat that was referred to the Gynecology Clinic of the Faculty of Veterinary Medicine, Selcuk University in 2010. In anamnesis, goat was a single kid at the birth and...

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Main Authors: Çağlayan Özel, Gonca Şen, Mustafa Hitit, Nadir Koçak, Aydın Güzeloğlu, Ahmet Semacan, Ercan Kurar, Ercan
Format: Article
Language:English
Published: Selcuk University Press
Series:Eurasian Journal of Veterinary Sciences
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Online Access:http://eurasianjvetsci.org/pdf.php3?id=1054
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author Çağlayan Özel
Gonca Şen
Mustafa Hitit
Nadir Koçak
Aydın Güzeloğlu
Ahmet Semacan
Ercan Kurar
Ercan
author_facet Çağlayan Özel
Gonca Şen
Mustafa Hitit
Nadir Koçak
Aydın Güzeloğlu
Ahmet Semacan
Ercan Kurar
Ercan
author_sort Çağlayan Özel
collection DOAJ
description This case report describes clinical examination and cytogenetic and molecular genetic analyses of a hermaphrodite mixed Maltese breed goat that was referred to the Gynecology Clinic of the Faculty of Veterinary Medicine, Selcuk University in 2010. In anamnesis, goat was a single kid at the birth and was polled. Clitoris was 1.9x0.9 cm in size and vagina was shorter than normal. By palpation and ultrasonographic examination, it was determined that the right (4.88 cm) and left (2.87 cm) testicles were located under skin in the inguinal region. Breast and nipples were in normal size. There was a rudimentary penis located under skin but no scrotum or prepitium was observed. After chromosome preparation using peripheral blood samples and GTG staining, appropriate metaphases were analyzed using an image analyzer program. Genomic DNA was isolated from the blood sample. Sexdetermining region Y (SRY) and amelogenin gene regions were amplified using polymerase chain reaction (PCR) including positive and negative controls. In karyotype analysis, metaphase plaque was observed as 60,XY. In PCR analysis, no SRY amplification was determined and only AMX allele was observed for amelogenin locus. Hermaphroditism is widely observed in goats and a number different mechanisms were reported. It is suggested that polled rams should be culled and karyotype and molecular genetics analyses should be performed to evaluate hermaphrodite cases in detail.
format Article
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institution DOAJ
issn 1309-6958
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language English
publisher Selcuk University Press
record_format Article
series Eurasian Journal of Veterinary Sciences
spelling doaj-art-71f905ed039d40dfa8ae4ceadfda33142025-08-20T02:52:06ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19533042272311054A case report of hermaphrodite goat: clinical and genetic approachÇağlayan Özel01Gonca Şen2Mustafa Hitit3Nadir Koçak4Aydın Güzeloğlu5Ahmet Semacan6Ercan Kurar7Ercan8Selçuk Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Konya, TürkiyeKırıkkale Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Kırıkkale, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Konya, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Konya, TürkiyeSelçuk Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Konya, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Konya, Türkiye Selçuk Üniversitesi, Veteriner Fakültesi, Doğum ve Jinekoloji Anabilim Dalı, Konya, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Konya, TürkiyeNecmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Konya, TürkiyeThis case report describes clinical examination and cytogenetic and molecular genetic analyses of a hermaphrodite mixed Maltese breed goat that was referred to the Gynecology Clinic of the Faculty of Veterinary Medicine, Selcuk University in 2010. In anamnesis, goat was a single kid at the birth and was polled. Clitoris was 1.9x0.9 cm in size and vagina was shorter than normal. By palpation and ultrasonographic examination, it was determined that the right (4.88 cm) and left (2.87 cm) testicles were located under skin in the inguinal region. Breast and nipples were in normal size. There was a rudimentary penis located under skin but no scrotum or prepitium was observed. After chromosome preparation using peripheral blood samples and GTG staining, appropriate metaphases were analyzed using an image analyzer program. Genomic DNA was isolated from the blood sample. Sexdetermining region Y (SRY) and amelogenin gene regions were amplified using polymerase chain reaction (PCR) including positive and negative controls. In karyotype analysis, metaphase plaque was observed as 60,XY. In PCR analysis, no SRY amplification was determined and only AMX allele was observed for amelogenin locus. Hermaphroditism is widely observed in goats and a number different mechanisms were reported. It is suggested that polled rams should be culled and karyotype and molecular genetics analyses should be performed to evaluate hermaphrodite cases in detail.http://eurasianjvetsci.org/pdf.php3?id=1054goathermaphroditismkaryotypesryamelogenin
spellingShingle Çağlayan Özel
Gonca Şen
Mustafa Hitit
Nadir Koçak
Aydın Güzeloğlu
Ahmet Semacan
Ercan Kurar
Ercan
A case report of hermaphrodite goat: clinical and genetic approach
Eurasian Journal of Veterinary Sciences
goat
hermaphroditism
karyotype
sry
amelogenin
title A case report of hermaphrodite goat: clinical and genetic approach
title_full A case report of hermaphrodite goat: clinical and genetic approach
title_fullStr A case report of hermaphrodite goat: clinical and genetic approach
title_full_unstemmed A case report of hermaphrodite goat: clinical and genetic approach
title_short A case report of hermaphrodite goat: clinical and genetic approach
title_sort case report of hermaphrodite goat clinical and genetic approach
topic goat
hermaphroditism
karyotype
sry
amelogenin
url http://eurasianjvetsci.org/pdf.php3?id=1054
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