Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery
Abstract Precision Cut Lung Slices (PCLS) have emerged as a sophisticated and physiologically relevant ex vivo model for studying the intricacies of lung diseases, including fibrosis, injury, repair, and host defense mechanisms. This innovative methodology presents a unique opportunity to bridge the...
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| Format: | Article |
| Language: | English |
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BMC
2024-06-01
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| Series: | Respiratory Research |
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| Online Access: | https://doi.org/10.1186/s12931-024-02855-6 |
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| author | Cynthia Koziol-White Eric Gebski Gaoyaun Cao Reynold A. Panettieri |
| author_facet | Cynthia Koziol-White Eric Gebski Gaoyaun Cao Reynold A. Panettieri |
| author_sort | Cynthia Koziol-White |
| collection | DOAJ |
| description | Abstract Precision Cut Lung Slices (PCLS) have emerged as a sophisticated and physiologically relevant ex vivo model for studying the intricacies of lung diseases, including fibrosis, injury, repair, and host defense mechanisms. This innovative methodology presents a unique opportunity to bridge the gap between traditional in vitro cell cultures and in vivo animal models, offering researchers a more accurate representation of the intricate microenvironment of the lung. PCLS require the precise sectioning of lung tissue to maintain its structural and functional integrity. These thin slices serve as invaluable tools for various research endeavors, particularly in the realm of airway diseases. By providing a controlled microenvironment, precision-cut lung slices empower researchers to dissect and comprehend the multifaceted interactions and responses within lung tissue, thereby advancing our understanding of pulmonary pathophysiology. |
| format | Article |
| id | doaj-art-71f7ddc284e4473cb3c3dcf06f33ca55 |
| institution | Kabale University |
| issn | 1465-993X |
| language | English |
| publishDate | 2024-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj-art-71f7ddc284e4473cb3c3dcf06f33ca552025-02-02T12:37:55ZengBMCRespiratory Research1465-993X2024-06-0125112110.1186/s12931-024-02855-6Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discoveryCynthia Koziol-White0Eric Gebski1Gaoyaun Cao2Reynold A. Panettieri3Rutgers Institute for Translational Medicine and Science, The State University of NJRutgers Institute for Translational Medicine and Science, The State University of NJRutgers Institute for Translational Medicine and Science, The State University of NJRutgers Institute for Translational Medicine and Science, The State University of NJAbstract Precision Cut Lung Slices (PCLS) have emerged as a sophisticated and physiologically relevant ex vivo model for studying the intricacies of lung diseases, including fibrosis, injury, repair, and host defense mechanisms. This innovative methodology presents a unique opportunity to bridge the gap between traditional in vitro cell cultures and in vivo animal models, offering researchers a more accurate representation of the intricate microenvironment of the lung. PCLS require the precise sectioning of lung tissue to maintain its structural and functional integrity. These thin slices serve as invaluable tools for various research endeavors, particularly in the realm of airway diseases. By providing a controlled microenvironment, precision-cut lung slices empower researchers to dissect and comprehend the multifaceted interactions and responses within lung tissue, thereby advancing our understanding of pulmonary pathophysiology.https://doi.org/10.1186/s12931-024-02855-6Precision cut lung slicesRespiratory therapeuticsAirway physiology in precision cut lung slices |
| spellingShingle | Cynthia Koziol-White Eric Gebski Gaoyaun Cao Reynold A. Panettieri Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery Respiratory Research Precision cut lung slices Respiratory therapeutics Airway physiology in precision cut lung slices |
| title | Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery |
| title_full | Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery |
| title_fullStr | Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery |
| title_full_unstemmed | Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery |
| title_short | Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery |
| title_sort | precision cut lung slices an integrated ex vivo model for studying lung physiology pharmacology disease pathogenesis and drug discovery |
| topic | Precision cut lung slices Respiratory therapeutics Airway physiology in precision cut lung slices |
| url | https://doi.org/10.1186/s12931-024-02855-6 |
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