Quiescent hepatic stellate cells functionally contribute to the hepatic innate immune response via TLR3.
Toll-like Receptor 3 (TLR3) is a pathogen pattern recognition receptor that plays a key role in innate immunity. TLR3 signalling has numerous functions in liver, both in health and disease. Here we report that TLR3 is expressed by quiescent hepatic stellate cells (HSC) where it functions to induce t...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0083391 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849331734246588416 |
|---|---|
| author | Caroline L Wilson Jelena Mann Meagan Walsh Maria J Perrugoria Fiona Oakley Matthew C Wright Chiara Brignole Daniela Di Paolo Patrizia Perri Mirco Ponzoni Michael Karin Derek A Mann |
| author_facet | Caroline L Wilson Jelena Mann Meagan Walsh Maria J Perrugoria Fiona Oakley Matthew C Wright Chiara Brignole Daniela Di Paolo Patrizia Perri Mirco Ponzoni Michael Karin Derek A Mann |
| author_sort | Caroline L Wilson |
| collection | DOAJ |
| description | Toll-like Receptor 3 (TLR3) is a pathogen pattern recognition receptor that plays a key role in innate immunity. TLR3 signalling has numerous functions in liver, both in health and disease. Here we report that TLR3 is expressed by quiescent hepatic stellate cells (HSC) where it functions to induce transcription and secretion of functional interferons as well as a number of other cytokines and chemokines. Upon transdifferentiation into myofibroblasts, HSCs rapidly loose the ability to produce interferon gamma (IFNγ). Mechanistically, this gene silencing may be due to Polycomb complex mediated repression via methylation of histone H3 lysine 27. In contrast to wild type, quiescent HSC isolated from tlr3 knockout mice do not produce IFNγ in response to Poly(I∶C) treatment. Therefore, quiescent HSC may contribute to induction of the hepatic innate immune system in response to injury or infection. |
| format | Article |
| id | doaj-art-71f7d585cd604254ace52ba7bc53da0f |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-71f7d585cd604254ace52ba7bc53da0f2025-08-20T03:46:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8339110.1371/journal.pone.0083391Quiescent hepatic stellate cells functionally contribute to the hepatic innate immune response via TLR3.Caroline L WilsonJelena MannMeagan WalshMaria J PerrugoriaFiona OakleyMatthew C WrightChiara BrignoleDaniela Di PaoloPatrizia PerriMirco PonzoniMichael KarinDerek A MannToll-like Receptor 3 (TLR3) is a pathogen pattern recognition receptor that plays a key role in innate immunity. TLR3 signalling has numerous functions in liver, both in health and disease. Here we report that TLR3 is expressed by quiescent hepatic stellate cells (HSC) where it functions to induce transcription and secretion of functional interferons as well as a number of other cytokines and chemokines. Upon transdifferentiation into myofibroblasts, HSCs rapidly loose the ability to produce interferon gamma (IFNγ). Mechanistically, this gene silencing may be due to Polycomb complex mediated repression via methylation of histone H3 lysine 27. In contrast to wild type, quiescent HSC isolated from tlr3 knockout mice do not produce IFNγ in response to Poly(I∶C) treatment. Therefore, quiescent HSC may contribute to induction of the hepatic innate immune system in response to injury or infection.https://doi.org/10.1371/journal.pone.0083391 |
| spellingShingle | Caroline L Wilson Jelena Mann Meagan Walsh Maria J Perrugoria Fiona Oakley Matthew C Wright Chiara Brignole Daniela Di Paolo Patrizia Perri Mirco Ponzoni Michael Karin Derek A Mann Quiescent hepatic stellate cells functionally contribute to the hepatic innate immune response via TLR3. PLoS ONE |
| title | Quiescent hepatic stellate cells functionally contribute to the hepatic innate immune response via TLR3. |
| title_full | Quiescent hepatic stellate cells functionally contribute to the hepatic innate immune response via TLR3. |
| title_fullStr | Quiescent hepatic stellate cells functionally contribute to the hepatic innate immune response via TLR3. |
| title_full_unstemmed | Quiescent hepatic stellate cells functionally contribute to the hepatic innate immune response via TLR3. |
| title_short | Quiescent hepatic stellate cells functionally contribute to the hepatic innate immune response via TLR3. |
| title_sort | quiescent hepatic stellate cells functionally contribute to the hepatic innate immune response via tlr3 |
| url | https://doi.org/10.1371/journal.pone.0083391 |
| work_keys_str_mv | AT carolinelwilson quiescenthepaticstellatecellsfunctionallycontributetothehepaticinnateimmuneresponseviatlr3 AT jelenamann quiescenthepaticstellatecellsfunctionallycontributetothehepaticinnateimmuneresponseviatlr3 AT meaganwalsh quiescenthepaticstellatecellsfunctionallycontributetothehepaticinnateimmuneresponseviatlr3 AT mariajperrugoria quiescenthepaticstellatecellsfunctionallycontributetothehepaticinnateimmuneresponseviatlr3 AT fionaoakley quiescenthepaticstellatecellsfunctionallycontributetothehepaticinnateimmuneresponseviatlr3 AT matthewcwright quiescenthepaticstellatecellsfunctionallycontributetothehepaticinnateimmuneresponseviatlr3 AT chiarabrignole quiescenthepaticstellatecellsfunctionallycontributetothehepaticinnateimmuneresponseviatlr3 AT danieladipaolo quiescenthepaticstellatecellsfunctionallycontributetothehepaticinnateimmuneresponseviatlr3 AT patriziaperri quiescenthepaticstellatecellsfunctionallycontributetothehepaticinnateimmuneresponseviatlr3 AT mircoponzoni quiescenthepaticstellatecellsfunctionallycontributetothehepaticinnateimmuneresponseviatlr3 AT michaelkarin quiescenthepaticstellatecellsfunctionallycontributetothehepaticinnateimmuneresponseviatlr3 AT derekamann quiescenthepaticstellatecellsfunctionallycontributetothehepaticinnateimmuneresponseviatlr3 |