Tumor-infiltrating lymphocytes in Wilms tumor

Purpose: T-lymphocytes-infiltrating tumors (TILs) are white blood cells (CD3+) found within the tumor and are thought to indicate a T-cell-mediated specific immune response. The purpose was to evaluate the presence and the subtypes of TILs in Wilms tumor (WT) specimens. Materials and Methods: A pros...

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Main Authors: Devendra Kumar Yadav, Vishesh Jain, Amit Kumar Dinda, Sandeep Agarwala
Format: Article
Language:English
Published: Thieme Medical and Scientific Publishers Pvt. Ltd. 2020-01-01
Series:Indian Journal of Medical and Paediatric Oncology
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Online Access:http://www.ijmpo.org/article.asp?issn=0971-5851;year=2020;volume=41;issue=1;spage=34;epage=38;aulast=Yadav
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author Devendra Kumar Yadav
Vishesh Jain
Amit Kumar Dinda
Sandeep Agarwala
author_facet Devendra Kumar Yadav
Vishesh Jain
Amit Kumar Dinda
Sandeep Agarwala
author_sort Devendra Kumar Yadav
collection DOAJ
description Purpose: T-lymphocytes-infiltrating tumors (TILs) are white blood cells (CD3+) found within the tumor and are thought to indicate a T-cell-mediated specific immune response. The purpose was to evaluate the presence and the subtypes of TILs in Wilms tumor (WT) specimens. Materials and Methods: A prospective study was conducted, and consecutive cases of WT registered and treated at our center were included in the study. The resected tumor was processed by taking multiple sections from the tumor and the peritumoral area. The sections were then processed for evaluating TILs and their subtypes (CD4 + and CD8+). TILs were assessed as a percentage of the total mononuclear cell infiltration in both these areas in all specimens. Results: Twenty-four consecutive specimens of WT were studied. The mean TILs (CD3+) counts was significantly higher (P = 0.001) in the peritumoral area (22.64 ± 1.42; range 20.2%–25%) as compared to the tumor (21.08 ± 2.42; range 14.6%–23.1%). The mean T-Helper (CD4+) counts was also significantly higher (P < 0.001) in the peritumoral area (13.04 ± 1.79; range 10.5%–16.5%) than in the tumor (7.30 ± 1.81; range 4.2%–10.1%). The mean cytolytic T-lymphocytes (CTLs-CD8+) counts in peritumoral area were 6.64 ± 1.09 (range 4.2%–9.2%), while in the tumor, it was 11.96 ± 3.09 (range 3.6%–16.4%). Two patients died on follow-up due to recurrence. These patients showed a markedly lower CD8+ cell count and higher CD4+/CD8+ ratio in the tumor. Conclusion: T-cells infiltration takes place in WT, and most of the patients demonstrate a higher concentration of CTL (CD8+) in the tumor as compared to the peritumoral area.
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spelling doaj-art-71f32302c4064c2abac6a8215a1dffac2025-08-20T01:55:40ZengThieme Medical and Scientific Publishers Pvt. Ltd.Indian Journal of Medical and Paediatric Oncology0971-58510975-21292020-01-01411343810.4103/ijmpo.ijmpo_115_19Tumor-infiltrating lymphocytes in Wilms tumorDevendra Kumar YadavVishesh JainAmit Kumar DindaSandeep AgarwalaPurpose: T-lymphocytes-infiltrating tumors (TILs) are white blood cells (CD3+) found within the tumor and are thought to indicate a T-cell-mediated specific immune response. The purpose was to evaluate the presence and the subtypes of TILs in Wilms tumor (WT) specimens. Materials and Methods: A prospective study was conducted, and consecutive cases of WT registered and treated at our center were included in the study. The resected tumor was processed by taking multiple sections from the tumor and the peritumoral area. The sections were then processed for evaluating TILs and their subtypes (CD4 + and CD8+). TILs were assessed as a percentage of the total mononuclear cell infiltration in both these areas in all specimens. Results: Twenty-four consecutive specimens of WT were studied. The mean TILs (CD3+) counts was significantly higher (P = 0.001) in the peritumoral area (22.64 ± 1.42; range 20.2%–25%) as compared to the tumor (21.08 ± 2.42; range 14.6%–23.1%). The mean T-Helper (CD4+) counts was also significantly higher (P < 0.001) in the peritumoral area (13.04 ± 1.79; range 10.5%–16.5%) than in the tumor (7.30 ± 1.81; range 4.2%–10.1%). The mean cytolytic T-lymphocytes (CTLs-CD8+) counts in peritumoral area were 6.64 ± 1.09 (range 4.2%–9.2%), while in the tumor, it was 11.96 ± 3.09 (range 3.6%–16.4%). Two patients died on follow-up due to recurrence. These patients showed a markedly lower CD8+ cell count and higher CD4+/CD8+ ratio in the tumor. Conclusion: T-cells infiltration takes place in WT, and most of the patients demonstrate a higher concentration of CTL (CD8+) in the tumor as compared to the peritumoral area.http://www.ijmpo.org/article.asp?issn=0971-5851;year=2020;volume=41;issue=1;spage=34;epage=38;aulast=Yadavimmunosurveillancetumor-infiltrating lymphocyteswilms tumor
spellingShingle Devendra Kumar Yadav
Vishesh Jain
Amit Kumar Dinda
Sandeep Agarwala
Tumor-infiltrating lymphocytes in Wilms tumor
Indian Journal of Medical and Paediatric Oncology
immunosurveillance
tumor-infiltrating lymphocytes
wilms tumor
title Tumor-infiltrating lymphocytes in Wilms tumor
title_full Tumor-infiltrating lymphocytes in Wilms tumor
title_fullStr Tumor-infiltrating lymphocytes in Wilms tumor
title_full_unstemmed Tumor-infiltrating lymphocytes in Wilms tumor
title_short Tumor-infiltrating lymphocytes in Wilms tumor
title_sort tumor infiltrating lymphocytes in wilms tumor
topic immunosurveillance
tumor-infiltrating lymphocytes
wilms tumor
url http://www.ijmpo.org/article.asp?issn=0971-5851;year=2020;volume=41;issue=1;spage=34;epage=38;aulast=Yadav
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AT visheshjain tumorinfiltratinglymphocytesinwilmstumor
AT amitkumardinda tumorinfiltratinglymphocytesinwilmstumor
AT sandeepagarwala tumorinfiltratinglymphocytesinwilmstumor