Expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult rats
The bone morphogenetic protein (BMP) pathway, which plays a crucial role in the control of intestinal epithelial cell homeostasis, has been studied in mice and humans, leading to an understanding of its involvement in several intestinal pathologies. However, the expression and localization of the v...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2025-08-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | https://www.ejh.it/ejh/article/view/4174 |
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| author | Emma Cogo Edwin Fouché Charline Buisson Adenike Omotoyinbo Fabrice Pierre Françoise Guéraud Pascale Plaisancié |
| author_facet | Emma Cogo Edwin Fouché Charline Buisson Adenike Omotoyinbo Fabrice Pierre Françoise Guéraud Pascale Plaisancié |
| author_sort | Emma Cogo |
| collection | DOAJ |
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The bone morphogenetic protein (BMP) pathway, which plays a crucial role in the control of intestinal epithelial cell homeostasis, has been studied in mice and humans, leading to an understanding of its involvement in several intestinal pathologies. However, the expression and localization of the various actors (ligands, antagonists, receptors) of this pathway remain unknown in the rat intestine, although this species is widely used in pathophysiology studies. Here, we aimed to determine the expression and localization of the various players in the BMP pathway in the jejunum and colon of the rat using RT-qPCR and immunohistochemistry. BMP2, mainly localized in epithelial cells, was the most expressed ligand in the jejunum and colon in comparison with BMP4, BMP6 and BMP7. We showed for the first time that BMP7 was highly expressed in epithelial cells in both tissues. BMP2, BMP6 and BMP7 ligands were also present in the enteric nervous plexuses, as the BMP receptors and antagonists Noggin and Chordin-like 1. The expression of BMP antagonists and ligands in enterocytes and mature colonocytes could suggest a paracrine or autocrine feedback modulation at the cellular level. Finally, all the studied BMP actors were present in colonic vessel walls including GREM1, a BMP antagonist described as pro-angiogenic and also being a ligand for VEGFR receptors. These data provided a good correlation between the observations in rats compared to those in humans and highlighted the importance of the BMP pathway not only in the intestinal epithelium, but also in both the enteric nervous system and vascular system. Our work lays the foundations for further studies on the involvement of the BMP pathway in rat models of intestinal pathophysiology.
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| format | Article |
| id | doaj-art-71f26119ecc9499f8d45b84a58b98f0a |
| institution | Kabale University |
| issn | 1121-760X 2038-8306 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj-art-71f26119ecc9499f8d45b84a58b98f0a2025-08-23T11:20:10ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062025-08-0169310.4081/ejh.2025.4174Expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult ratsEmma Cogo0Edwin Fouché1Charline Buisson2Adenike Omotoyinbo3Fabrice Pierre4Françoise Guéraud5Pascale Plaisancié6Toxalim (Research Centre in Food Toxicology), Toulouse University; INRAE UMR 1331, ENVT, INP-Purpan, Paul Sabatier University, ToulouseToxalim (Research Centre in Food Toxicology), Toulouse University; INRAE UMR 1331, ENVT, INP-Purpan, Paul Sabatier University, ToulouseToxalim (Research Centre in Food Toxicology), Toulouse University; INRAE UMR 1331, ENVT, INP-Purpan, Paul Sabatier University, ToulouseToxalim (Research Centre in Food Toxicology), Toulouse University; INRAE UMR 1331, ENVT, INP-Purpan, Paul Sabatier University, ToulouseToxalim (Research Centre in Food Toxicology), Toulouse University; INRAE UMR 1331, ENVT, INP-Purpan, Paul Sabatier University, ToulouseToxalim (Research Centre in Food Toxicology), Toulouse University; INRAE UMR 1331, ENVT, INP-Purpan, Paul Sabatier University, ToulouseToxalim (Research Centre in Food Toxicology), Toulouse University; INRAE UMR 1331, ENVT, INP-Purpan, Paul Sabatier University, Toulouse The bone morphogenetic protein (BMP) pathway, which plays a crucial role in the control of intestinal epithelial cell homeostasis, has been studied in mice and humans, leading to an understanding of its involvement in several intestinal pathologies. However, the expression and localization of the various actors (ligands, antagonists, receptors) of this pathway remain unknown in the rat intestine, although this species is widely used in pathophysiology studies. Here, we aimed to determine the expression and localization of the various players in the BMP pathway in the jejunum and colon of the rat using RT-qPCR and immunohistochemistry. BMP2, mainly localized in epithelial cells, was the most expressed ligand in the jejunum and colon in comparison with BMP4, BMP6 and BMP7. We showed for the first time that BMP7 was highly expressed in epithelial cells in both tissues. BMP2, BMP6 and BMP7 ligands were also present in the enteric nervous plexuses, as the BMP receptors and antagonists Noggin and Chordin-like 1. The expression of BMP antagonists and ligands in enterocytes and mature colonocytes could suggest a paracrine or autocrine feedback modulation at the cellular level. Finally, all the studied BMP actors were present in colonic vessel walls including GREM1, a BMP antagonist described as pro-angiogenic and also being a ligand for VEGFR receptors. These data provided a good correlation between the observations in rats compared to those in humans and highlighted the importance of the BMP pathway not only in the intestinal epithelium, but also in both the enteric nervous system and vascular system. Our work lays the foundations for further studies on the involvement of the BMP pathway in rat models of intestinal pathophysiology. https://www.ejh.it/ejh/article/view/4174BMP pathwayligandsantagonistsreceptorsadult ratjejunum |
| spellingShingle | Emma Cogo Edwin Fouché Charline Buisson Adenike Omotoyinbo Fabrice Pierre Françoise Guéraud Pascale Plaisancié Expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult rats European Journal of Histochemistry BMP pathway ligands antagonists receptors adult rat jejunum |
| title | Expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult rats |
| title_full | Expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult rats |
| title_fullStr | Expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult rats |
| title_full_unstemmed | Expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult rats |
| title_short | Expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult rats |
| title_sort | expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult rats |
| topic | BMP pathway ligands antagonists receptors adult rat jejunum |
| url | https://www.ejh.it/ejh/article/view/4174 |
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