Plasma Extracellular Vesicles Biomarkers Linked to Lower Muscle Mass, Function and Physical Performance in Sarcopenia
ABSTRACT Background As society ages, identifying individuals at risk of sarcopenia becomes essential. Several plasma biomarkers are used to assess musculoskeletal status, but their results are inconsistent. Extracellular vesicles (EVs) are investigated as disease biomarkers due to their role in tran...
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| Format: | Article |
| Language: | English |
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Wiley
2025-04-01
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| Series: | Journal of Cachexia, Sarcopenia and Muscle |
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| Online Access: | https://doi.org/10.1002/jcsm.13784 |
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| author | Ji Yeon Kim Tae‐Hwan Gil Hyo Gyeong Lee Ji‐Won Shin Dong‐Hyun Jang Hyeon Soo Kim Seung Shin Park Sang Wan Kim Chan Soo Shin Sung Hye Kong Ok Hee Jeon |
| author_facet | Ji Yeon Kim Tae‐Hwan Gil Hyo Gyeong Lee Ji‐Won Shin Dong‐Hyun Jang Hyeon Soo Kim Seung Shin Park Sang Wan Kim Chan Soo Shin Sung Hye Kong Ok Hee Jeon |
| author_sort | Ji Yeon Kim |
| collection | DOAJ |
| description | ABSTRACT Background As society ages, identifying individuals at risk of sarcopenia becomes essential. Several plasma biomarkers are used to assess musculoskeletal status, but their results are inconsistent. Extracellular vesicles (EVs) are investigated as disease biomarkers due to their role in transporting molecules and influencing cellular processes. This study investigated the correlation of known sarcopenia biomarkers—adiponectin, myostatin, P3NP, CRP and TNF‐α—measured from plasma‐derived EVs with muscle mass, function and performance in an Osteoporosis Sarcopenia cohort at the Seoul National University Bundang Hospital. Methods Muscle mass was evaluated by measuring appendicular skeletal muscle mass (ASM) using dual X‐ray absorptiometry and calculated as ASM/height2. Hand grip strength was measured using a hydraulic hand dynamometer for muscle function and physical performance based on the Short Physical Performance Battery (SPPB), walking speed and the five‐time‐sit‐to‐stand test. Density gradient ultracentrifugation was used to isolate EVs from the plasma, followed by confirming the expression of sarcopenia biomarkers. Multivariate regression analysis, adjusted for sex, age, body mass index, smoking, drinking, and bone density, was performed. Results The mean age of participants was 74.3 ± 12.1 years (range, 52.0–96.0), with 88.2% being female. Plasma‐derived EV levels of myostatin and P3NP were significantly associated with walking speed (ꞵ = −0.309, p = 0.014) and SPPB (ꞵ = −0.276, p = 0.029), respectively. TNF‐α levels were strongly correlated with hand grip strength (ꞵ = −0.313, p = 0.013). Using receiver‐operating characteristic curve analysis, cutoff values for three factors were determined, allowing participants to be categorized into high and low groups. Low myostatin group had a higher hand grip strength (19.63 kg vs. 17.14 kg, p = 0.027) and faster five‐time‐sit‐to‐stand test times (17.34 s vs. 23.72 s, p = 0.032). Low P3NP levels showed a stronger grip strength (19.87 kg vs. 16.81 kg, p = 0.008), better SPPB scores (9.10 vs. 8.03, p = 0.006) and five‐time‐sit‐to‐stand times (18.31 s vs. 21.87 s, p = 0.002). Low TNF‐α levels were linked to better walking speeds (0.82 m/s vs. 0.64 m/s, p = 0.009) and lower SARC‐F scores (1.73 vs. 3.26, p = 0.029). Conclusion Our research confirmed that EVs‐derived myostatin, P3NP and TNF‐α are strongly associated with muscle function and performance. Significant differences in these factors between high and low groups based on biomarker cutoffs emphasize their diagnostic relevance for sarcopenia. These findings offer a promising avenue for identifying effective markers in future research and clinical applications. |
| format | Article |
| id | doaj-art-71f0ea9045ac444a82efac7e2b467c61 |
| institution | Kabale University |
| issn | 2190-5991 2190-6009 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
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| series | Journal of Cachexia, Sarcopenia and Muscle |
| spelling | doaj-art-71f0ea9045ac444a82efac7e2b467c612025-08-20T03:46:37ZengWileyJournal of Cachexia, Sarcopenia and Muscle2190-59912190-60092025-04-01162n/an/a10.1002/jcsm.13784Plasma Extracellular Vesicles Biomarkers Linked to Lower Muscle Mass, Function and Physical Performance in SarcopeniaJi Yeon Kim0Tae‐Hwan Gil1Hyo Gyeong Lee2Ji‐Won Shin3Dong‐Hyun Jang4Hyeon Soo Kim5Seung Shin Park6Sang Wan Kim7Chan Soo Shin8Sung Hye Kong9Ok Hee Jeon10Department of Biomedical Sciences Korea University College of Medicine Seoul Republic of KoreaDepartment of Biomedical Sciences Korea University College of Medicine Seoul Republic of KoreaDepartment of Biomedical Sciences Korea University College of Medicine Seoul Republic of KoreaDepartment of Biomedical Sciences Korea University College of Medicine Seoul Republic of KoreaDepartment of Biomedical Sciences Korea University College of Medicine Seoul Republic of KoreaDepartment of Biomedical Sciences Korea University College of Medicine Seoul Republic of KoreaDepartment of Internal Medicine Seoul National University Hospital Seoul Republic of KoreaDepartment of Internal Medicine Seoul National University Boramae Hospital Seoul Republic of KoreaDepartment of Internal Medicine Seoul National University Hospital Seoul Republic of KoreaDepartment of Internal Medicine Seoul National University College of Medicine Seoul Republic of KoreaDepartment of Biomedical Sciences Korea University College of Medicine Seoul Republic of KoreaABSTRACT Background As society ages, identifying individuals at risk of sarcopenia becomes essential. Several plasma biomarkers are used to assess musculoskeletal status, but their results are inconsistent. Extracellular vesicles (EVs) are investigated as disease biomarkers due to their role in transporting molecules and influencing cellular processes. This study investigated the correlation of known sarcopenia biomarkers—adiponectin, myostatin, P3NP, CRP and TNF‐α—measured from plasma‐derived EVs with muscle mass, function and performance in an Osteoporosis Sarcopenia cohort at the Seoul National University Bundang Hospital. Methods Muscle mass was evaluated by measuring appendicular skeletal muscle mass (ASM) using dual X‐ray absorptiometry and calculated as ASM/height2. Hand grip strength was measured using a hydraulic hand dynamometer for muscle function and physical performance based on the Short Physical Performance Battery (SPPB), walking speed and the five‐time‐sit‐to‐stand test. Density gradient ultracentrifugation was used to isolate EVs from the plasma, followed by confirming the expression of sarcopenia biomarkers. Multivariate regression analysis, adjusted for sex, age, body mass index, smoking, drinking, and bone density, was performed. Results The mean age of participants was 74.3 ± 12.1 years (range, 52.0–96.0), with 88.2% being female. Plasma‐derived EV levels of myostatin and P3NP were significantly associated with walking speed (ꞵ = −0.309, p = 0.014) and SPPB (ꞵ = −0.276, p = 0.029), respectively. TNF‐α levels were strongly correlated with hand grip strength (ꞵ = −0.313, p = 0.013). Using receiver‐operating characteristic curve analysis, cutoff values for three factors were determined, allowing participants to be categorized into high and low groups. Low myostatin group had a higher hand grip strength (19.63 kg vs. 17.14 kg, p = 0.027) and faster five‐time‐sit‐to‐stand test times (17.34 s vs. 23.72 s, p = 0.032). Low P3NP levels showed a stronger grip strength (19.87 kg vs. 16.81 kg, p = 0.008), better SPPB scores (9.10 vs. 8.03, p = 0.006) and five‐time‐sit‐to‐stand times (18.31 s vs. 21.87 s, p = 0.002). Low TNF‐α levels were linked to better walking speeds (0.82 m/s vs. 0.64 m/s, p = 0.009) and lower SARC‐F scores (1.73 vs. 3.26, p = 0.029). Conclusion Our research confirmed that EVs‐derived myostatin, P3NP and TNF‐α are strongly associated with muscle function and performance. Significant differences in these factors between high and low groups based on biomarker cutoffs emphasize their diagnostic relevance for sarcopenia. These findings offer a promising avenue for identifying effective markers in future research and clinical applications.https://doi.org/10.1002/jcsm.13784ageingbiomarkersextracellular vesiclesarcopeniaskeletal muscle |
| spellingShingle | Ji Yeon Kim Tae‐Hwan Gil Hyo Gyeong Lee Ji‐Won Shin Dong‐Hyun Jang Hyeon Soo Kim Seung Shin Park Sang Wan Kim Chan Soo Shin Sung Hye Kong Ok Hee Jeon Plasma Extracellular Vesicles Biomarkers Linked to Lower Muscle Mass, Function and Physical Performance in Sarcopenia Journal of Cachexia, Sarcopenia and Muscle ageing biomarkers extracellular vesicle sarcopenia skeletal muscle |
| title | Plasma Extracellular Vesicles Biomarkers Linked to Lower Muscle Mass, Function and Physical Performance in Sarcopenia |
| title_full | Plasma Extracellular Vesicles Biomarkers Linked to Lower Muscle Mass, Function and Physical Performance in Sarcopenia |
| title_fullStr | Plasma Extracellular Vesicles Biomarkers Linked to Lower Muscle Mass, Function and Physical Performance in Sarcopenia |
| title_full_unstemmed | Plasma Extracellular Vesicles Biomarkers Linked to Lower Muscle Mass, Function and Physical Performance in Sarcopenia |
| title_short | Plasma Extracellular Vesicles Biomarkers Linked to Lower Muscle Mass, Function and Physical Performance in Sarcopenia |
| title_sort | plasma extracellular vesicles biomarkers linked to lower muscle mass function and physical performance in sarcopenia |
| topic | ageing biomarkers extracellular vesicle sarcopenia skeletal muscle |
| url | https://doi.org/10.1002/jcsm.13784 |
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