Plant genotype-specific modulation of Clonostachys rosea-mediated biocontrol of septoria tritici blotch disease in wheat
Abstract Background Beneficial microorganisms can act as biological control agents (BCAs) directly by targeting pathogens or indirectly by enhancing the plant’s defense mechanisms against pathogens. However, efficiencies with which plants benefit from BCAs vary, potentially because of genetic variat...
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2025-05-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-06620-9 |
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| author | Sidhant Chaudhary Mustafa Zakieh Mukesh Dubey Dan Funck Jensen Laura Grenville-Briggs Aakash Chawade Magnus Karlsson |
| author_facet | Sidhant Chaudhary Mustafa Zakieh Mukesh Dubey Dan Funck Jensen Laura Grenville-Briggs Aakash Chawade Magnus Karlsson |
| author_sort | Sidhant Chaudhary |
| collection | DOAJ |
| description | Abstract Background Beneficial microorganisms can act as biological control agents (BCAs) directly by targeting pathogens or indirectly by enhancing the plant’s defense mechanisms against pathogens. However, efficiencies with which plants benefit from BCAs vary, potentially because of genetic variation in plants for plant-BCA compatibility. The aim of this study was to explore the genetic variation in winter wheat for modulation of Clonostachys rosea-mediated biocontrol of septoria tritici blotch disease caused by the fungal pathogen Zymoseptoria tritici. Results In total, 202 winter wheat genotypes, including landraces and old cultivars grown from 1900 onwards in the Scandinavian countries, were tested under greenhouse-controlled conditions. Foliar spray applications of the pathogen and the fungal BCA in two treatments, i.e., Z. tritici (Zt) alone and Z. tritici along with C. rosea (ZtCr) were used to assess the disease progress over time. The absence and presence of C. rosea in Zt and ZtCr, respectively, allowed the dissection of variation for plant disease resistance and biocontrol efficacy. The study showed significant (P < 0.05) phenotypic variation among plant genotypes for disease progression in both Zt and ZtCr treatments. Moreover, the application of C. rosea resulted in a significant (P < 0.05) reduction in disease progression for seven genotypes and increased disease progression for eleven genotypes, indicating a plant genotype-dependent effect on the interaction between wheat, C. rosea and Z. tritici. For the phenotypic variation in disease progress and biocontrol efficacy, a genome-wide association study using a 20K single-nucleotide polymorphism (SNP) marker array was also performed. In total, five distinct SNP markers associated with disease resistance and four SNP markers associated with C. rosea biocontrol efficacy were identified. Conclusions This work serves as a foundation to further characterize the genetic basis of plant-BCA interactions when inoculated with Z. tritici, facilitating opportunities for simultaneous breeding for disease resistance and biocontrol efficacy. |
| format | Article |
| id | doaj-art-71ed897e89274ad4a4debacf1f9b5f18 |
| institution | OA Journals |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj-art-71ed897e89274ad4a4debacf1f9b5f182025-08-20T01:47:33ZengBMCBMC Plant Biology1471-22292025-05-0125111810.1186/s12870-025-06620-9Plant genotype-specific modulation of Clonostachys rosea-mediated biocontrol of septoria tritici blotch disease in wheatSidhant Chaudhary0Mustafa Zakieh1Mukesh Dubey2Dan Funck Jensen3Laura Grenville-Briggs4Aakash Chawade5Magnus Karlsson6Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural SciencesDepartment of Plant Breeding, Swedish University of Agricultural SciencesDepartment of Forest Mycology and Plant Pathology, Swedish University of Agricultural SciencesDepartment of Forest Mycology and Plant Pathology, Swedish University of Agricultural SciencesDepartment of Plant Protection Biology, Swedish University of Agricultural SciencesDepartment of Plant Breeding, Swedish University of Agricultural SciencesDepartment of Forest Mycology and Plant Pathology, Swedish University of Agricultural SciencesAbstract Background Beneficial microorganisms can act as biological control agents (BCAs) directly by targeting pathogens or indirectly by enhancing the plant’s defense mechanisms against pathogens. However, efficiencies with which plants benefit from BCAs vary, potentially because of genetic variation in plants for plant-BCA compatibility. The aim of this study was to explore the genetic variation in winter wheat for modulation of Clonostachys rosea-mediated biocontrol of septoria tritici blotch disease caused by the fungal pathogen Zymoseptoria tritici. Results In total, 202 winter wheat genotypes, including landraces and old cultivars grown from 1900 onwards in the Scandinavian countries, were tested under greenhouse-controlled conditions. Foliar spray applications of the pathogen and the fungal BCA in two treatments, i.e., Z. tritici (Zt) alone and Z. tritici along with C. rosea (ZtCr) were used to assess the disease progress over time. The absence and presence of C. rosea in Zt and ZtCr, respectively, allowed the dissection of variation for plant disease resistance and biocontrol efficacy. The study showed significant (P < 0.05) phenotypic variation among plant genotypes for disease progression in both Zt and ZtCr treatments. Moreover, the application of C. rosea resulted in a significant (P < 0.05) reduction in disease progression for seven genotypes and increased disease progression for eleven genotypes, indicating a plant genotype-dependent effect on the interaction between wheat, C. rosea and Z. tritici. For the phenotypic variation in disease progress and biocontrol efficacy, a genome-wide association study using a 20K single-nucleotide polymorphism (SNP) marker array was also performed. In total, five distinct SNP markers associated with disease resistance and four SNP markers associated with C. rosea biocontrol efficacy were identified. Conclusions This work serves as a foundation to further characterize the genetic basis of plant-BCA interactions when inoculated with Z. tritici, facilitating opportunities for simultaneous breeding for disease resistance and biocontrol efficacy.https://doi.org/10.1186/s12870-025-06620-9Biological control agentClonostachys roseaGenome-wide association studyIntegrated pest managementSeptoria tritici blotchSingle nucleotide polymorphism |
| spellingShingle | Sidhant Chaudhary Mustafa Zakieh Mukesh Dubey Dan Funck Jensen Laura Grenville-Briggs Aakash Chawade Magnus Karlsson Plant genotype-specific modulation of Clonostachys rosea-mediated biocontrol of septoria tritici blotch disease in wheat BMC Plant Biology Biological control agent Clonostachys rosea Genome-wide association study Integrated pest management Septoria tritici blotch Single nucleotide polymorphism |
| title | Plant genotype-specific modulation of Clonostachys rosea-mediated biocontrol of septoria tritici blotch disease in wheat |
| title_full | Plant genotype-specific modulation of Clonostachys rosea-mediated biocontrol of septoria tritici blotch disease in wheat |
| title_fullStr | Plant genotype-specific modulation of Clonostachys rosea-mediated biocontrol of septoria tritici blotch disease in wheat |
| title_full_unstemmed | Plant genotype-specific modulation of Clonostachys rosea-mediated biocontrol of septoria tritici blotch disease in wheat |
| title_short | Plant genotype-specific modulation of Clonostachys rosea-mediated biocontrol of septoria tritici blotch disease in wheat |
| title_sort | plant genotype specific modulation of clonostachys rosea mediated biocontrol of septoria tritici blotch disease in wheat |
| topic | Biological control agent Clonostachys rosea Genome-wide association study Integrated pest management Septoria tritici blotch Single nucleotide polymorphism |
| url | https://doi.org/10.1186/s12870-025-06620-9 |
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