A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol
An efficient, sensitive, and low-cost method has been developed for turn-on fluorescence sensing of dopamine (DA). The method relies on the rapid reaction of DA and 3-Hydroxyphenylboronic acid (3-HPBA) via specific recognition between boronic acids and cis-diol of DA in alkaline solution. The reacti...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/6540397 |
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| author | Xiaoxia Liu Miaomiao Tian Wenmei Gao Jinzhong Zhao |
| author_facet | Xiaoxia Liu Miaomiao Tian Wenmei Gao Jinzhong Zhao |
| author_sort | Xiaoxia Liu |
| collection | DOAJ |
| description | An efficient, sensitive, and low-cost method has been developed for turn-on fluorescence sensing of dopamine (DA). The method relies on the rapid reaction of DA and 3-Hydroxyphenylboronic acid (3-HPBA) via specific recognition between boronic acids and cis-diol of DA in alkaline solution. The reaction product shows an excitation wavelength of 417 nm and the maximum emission peak at 470 nm. The proposed method allows the determination of DA in the range of 50 nM–25 μM, and the whole detection can be completed within 5 minutes. Furthermore, the presented approach has good selectivity and has been successfully applied to DA sensing in human serum samples, showing great potential in clinical diagnosis. |
| format | Article |
| id | doaj-art-71ecc1bd0ea640bba113583d3708755d |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-71ecc1bd0ea640bba113583d3708755d2025-08-20T03:33:45ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732019-01-01201910.1155/2019/65403976540397A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-DiolXiaoxia Liu0Miaomiao Tian1Wenmei Gao2Jinzhong Zhao3College of Arts and Sciences, Shanxi Agricultural University, Jinzhong, Shanxi 030801, ChinaKey Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, Changchun, Jilin 130024, ChinaCollege of Arts and Sciences, Shanxi Agricultural University, Jinzhong, Shanxi 030801, ChinaCollege of Arts and Sciences, Shanxi Agricultural University, Jinzhong, Shanxi 030801, ChinaAn efficient, sensitive, and low-cost method has been developed for turn-on fluorescence sensing of dopamine (DA). The method relies on the rapid reaction of DA and 3-Hydroxyphenylboronic acid (3-HPBA) via specific recognition between boronic acids and cis-diol of DA in alkaline solution. The reaction product shows an excitation wavelength of 417 nm and the maximum emission peak at 470 nm. The proposed method allows the determination of DA in the range of 50 nM–25 μM, and the whole detection can be completed within 5 minutes. Furthermore, the presented approach has good selectivity and has been successfully applied to DA sensing in human serum samples, showing great potential in clinical diagnosis.http://dx.doi.org/10.1155/2019/6540397 |
| spellingShingle | Xiaoxia Liu Miaomiao Tian Wenmei Gao Jinzhong Zhao A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol Journal of Analytical Methods in Chemistry |
| title | A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol |
| title_full | A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol |
| title_fullStr | A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol |
| title_full_unstemmed | A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol |
| title_short | A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol |
| title_sort | simple rapid fluorometric assay for dopamine by in situ reaction of boronic acids and cis diol |
| url | http://dx.doi.org/10.1155/2019/6540397 |
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