Polymerase chain reaction in viral anterior uveitis with secondary glaucoma: Analysis of 28 eyes from a tertiary eye care center in South India
PURPOSE: To study the role of polymerase chain reaction (PCR) in viral anterior uveitis with secondary glaucoma. MATERIALS AND METHODS: This was a retrospective cohort study in which aqueous humor from 28 eyes of 23 patients with viral uveitis and secondary glaucoma from 2008 to 2024 was sent for re...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-04-01
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| Series: | Taiwan Journal of Ophthalmology |
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| Online Access: | https://journals.lww.com/10.4103/tjo.TJO-D-24-00089 |
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| author | Bhavini Johri Dhanurekha Lakshmipathy Jyotirmay Biswas |
| author_facet | Bhavini Johri Dhanurekha Lakshmipathy Jyotirmay Biswas |
| author_sort | Bhavini Johri |
| collection | DOAJ |
| description | PURPOSE:
To study the role of polymerase chain reaction (PCR) in viral anterior uveitis with secondary glaucoma.
MATERIALS AND METHODS:
This was a retrospective cohort study in which aqueous humor from 28 eyes of 23 patients with viral uveitis and secondary glaucoma from 2008 to 2024 was sent for real-time/nested PCR. The clinical features of the patients were analyzed (P < 0.05 statistically significant).
RESULTS:
The median age at uveitis onset was 45 years. Secondary open-angle glaucoma was found in 23 out of 28 eyes, 82.1%. The median intraocular pressure (IOP) reduced significantly from baseline, 18.5 to 14 mmHg at the final visit (P = 0.01). Maximum IOP was 60 mmHg. PCR was positive in 57.1% of eyes, mostly for varicella-zoster virus (32.1%), followed by Cytomegalovirus (CMV) (17.8%) and herpes simplex virus (10.7%). Valacyclovir was used to treat 50% of eyes. CMV was treated with valganciclovir (10.7% eyes). Antiglaucoma and anti-inflammatory medications were reduced significantly from initiation (P < 0.001).
CONCLUSION:
PCR enables timely and accurate detection of virus causing anterior uveitis and helps salvage vision in secondary glaucoma with appropriate antiviral therapy. |
| format | Article |
| id | doaj-art-71e447b4d3fe4fa1a9f2f937fe566dfe |
| institution | DOAJ |
| issn | 2211-5056 2211-5072 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Taiwan Journal of Ophthalmology |
| spelling | doaj-art-71e447b4d3fe4fa1a9f2f937fe566dfe2025-08-20T03:16:11ZengWolters Kluwer Medknow PublicationsTaiwan Journal of Ophthalmology2211-50562211-50722025-04-0115228328910.4103/tjo.TJO-D-24-00089Polymerase chain reaction in viral anterior uveitis with secondary glaucoma: Analysis of 28 eyes from a tertiary eye care center in South IndiaBhavini JohriDhanurekha LakshmipathyJyotirmay BiswasPURPOSE: To study the role of polymerase chain reaction (PCR) in viral anterior uveitis with secondary glaucoma. MATERIALS AND METHODS: This was a retrospective cohort study in which aqueous humor from 28 eyes of 23 patients with viral uveitis and secondary glaucoma from 2008 to 2024 was sent for real-time/nested PCR. The clinical features of the patients were analyzed (P < 0.05 statistically significant). RESULTS: The median age at uveitis onset was 45 years. Secondary open-angle glaucoma was found in 23 out of 28 eyes, 82.1%. The median intraocular pressure (IOP) reduced significantly from baseline, 18.5 to 14 mmHg at the final visit (P = 0.01). Maximum IOP was 60 mmHg. PCR was positive in 57.1% of eyes, mostly for varicella-zoster virus (32.1%), followed by Cytomegalovirus (CMV) (17.8%) and herpes simplex virus (10.7%). Valacyclovir was used to treat 50% of eyes. CMV was treated with valganciclovir (10.7% eyes). Antiglaucoma and anti-inflammatory medications were reduced significantly from initiation (P < 0.001). CONCLUSION: PCR enables timely and accurate detection of virus causing anterior uveitis and helps salvage vision in secondary glaucoma with appropriate antiviral therapy.https://journals.lww.com/10.4103/tjo.TJO-D-24-00089polymerase chain reactionsecondary glaucomaviral anterior uveitis |
| spellingShingle | Bhavini Johri Dhanurekha Lakshmipathy Jyotirmay Biswas Polymerase chain reaction in viral anterior uveitis with secondary glaucoma: Analysis of 28 eyes from a tertiary eye care center in South India Taiwan Journal of Ophthalmology polymerase chain reaction secondary glaucoma viral anterior uveitis |
| title | Polymerase chain reaction in viral anterior uveitis with secondary glaucoma: Analysis of 28 eyes from a tertiary eye care center in South India |
| title_full | Polymerase chain reaction in viral anterior uveitis with secondary glaucoma: Analysis of 28 eyes from a tertiary eye care center in South India |
| title_fullStr | Polymerase chain reaction in viral anterior uveitis with secondary glaucoma: Analysis of 28 eyes from a tertiary eye care center in South India |
| title_full_unstemmed | Polymerase chain reaction in viral anterior uveitis with secondary glaucoma: Analysis of 28 eyes from a tertiary eye care center in South India |
| title_short | Polymerase chain reaction in viral anterior uveitis with secondary glaucoma: Analysis of 28 eyes from a tertiary eye care center in South India |
| title_sort | polymerase chain reaction in viral anterior uveitis with secondary glaucoma analysis of 28 eyes from a tertiary eye care center in south india |
| topic | polymerase chain reaction secondary glaucoma viral anterior uveitis |
| url | https://journals.lww.com/10.4103/tjo.TJO-D-24-00089 |
| work_keys_str_mv | AT bhavinijohri polymerasechainreactioninviralanterioruveitiswithsecondaryglaucomaanalysisof28eyesfromatertiaryeyecarecenterinsouthindia AT dhanurekhalakshmipathy polymerasechainreactioninviralanterioruveitiswithsecondaryglaucomaanalysisof28eyesfromatertiaryeyecarecenterinsouthindia AT jyotirmaybiswas polymerasechainreactioninviralanterioruveitiswithsecondaryglaucomaanalysisof28eyesfromatertiaryeyecarecenterinsouthindia |