Silver nanoparticles biosynthesized from Stenocereus queretaroensis with antiproliferative activity
Aim: This work aimed to evaluate the antiproliferative activity of silver nanoparticles (AgNPs) biosynthesized with aqueous extract of Stenocereus queretaroensis peel (SAgNPs) in pancreatic ductal cancer cells PANC-1. Methods: Nanoparticles were synthesized using 2 mM silver nitrate (AgNO3) and a 1%...
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Open Exploration Publishing Inc.
2025-02-01
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| Series: | Exploration of BioMat-X |
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| Online Access: | https://www.explorationpub.com/uploads/Article/A101328/101328.pdf |
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| author | Ariadna Abigail Villarreal-Amézquita Ivan Moises Sánchez-Hernández Eduardo Padilla-Camberos |
| author_facet | Ariadna Abigail Villarreal-Amézquita Ivan Moises Sánchez-Hernández Eduardo Padilla-Camberos |
| author_sort | Ariadna Abigail Villarreal-Amézquita |
| collection | DOAJ |
| description | Aim: This work aimed to evaluate the antiproliferative activity of silver nanoparticles (AgNPs) biosynthesized with aqueous extract of Stenocereus queretaroensis peel (SAgNPs) in pancreatic ductal cancer cells PANC-1. Methods: Nanoparticles were synthesized using 2 mM silver nitrate (AgNO3) and a 1% aqueous extract of Stenocereus queretaroensis peel. SAgNPs were characterized by ultraviolet-visible spectroscopy (UV-Vis) light spectroscopy, dynamic light scattering analysis, and transmission electron microscopy. The antiproliferative activity was evaluated in the PANC-1 cell line by measuring the viability percentage with the 3'-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method and subsequently the IC50 of SAgNPs. Results: The presence of AgNPs was confirmed by silver surface plasmon resonance at 420 nm. The average size obtained by dynamic light scattering analysis was 98.96 nm, with a spherical and uniform shape according to transmission electron microscopy analysis. SAgNPs were tested at concentrations from 10 µg/mL to 0.3125 µg/mL and presented inhibition percentages from 3.76% to 90.09% with an IC50 value of 3.04 µg/mL (p-value of 0.02, 95% confidence level) in PANC-1 cells. Conclusions: The biologically synthesized nanoparticles with Stenocereus queretaroensis peel showed antiproliferative activity in PANC-1 pancreatic cancer cells. Therefore, these results suggest their potential use in the prevention and treatment of pancreatic cancer with further investigation. |
| format | Article |
| id | doaj-art-71e19ba19b454634ad962e2fee348eca |
| institution | Kabale University |
| issn | 2996-9476 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Open Exploration Publishing Inc. |
| record_format | Article |
| series | Exploration of BioMat-X |
| spelling | doaj-art-71e19ba19b454634ad962e2fee348eca2025-08-20T03:27:25ZengOpen Exploration Publishing Inc.Exploration of BioMat-X2996-94762025-02-012552810.37349/ebmx.2025.101328Silver nanoparticles biosynthesized from Stenocereus queretaroensis with antiproliferative activityAriadna Abigail Villarreal-Amézquita0https://orcid.org/0009-0009-0224-1805Ivan Moises Sánchez-Hernández1https://orcid.org/0000-0001-7328-0048Eduardo Padilla-Camberos2https://orcid.org/0000-0003-4783-841XUnit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Guadalajara 44270, Jalisco, MexicoUnit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Guadalajara 44270, Jalisco, MexicoUnit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Guadalajara 44270, Jalisco, MexicoAim: This work aimed to evaluate the antiproliferative activity of silver nanoparticles (AgNPs) biosynthesized with aqueous extract of Stenocereus queretaroensis peel (SAgNPs) in pancreatic ductal cancer cells PANC-1. Methods: Nanoparticles were synthesized using 2 mM silver nitrate (AgNO3) and a 1% aqueous extract of Stenocereus queretaroensis peel. SAgNPs were characterized by ultraviolet-visible spectroscopy (UV-Vis) light spectroscopy, dynamic light scattering analysis, and transmission electron microscopy. The antiproliferative activity was evaluated in the PANC-1 cell line by measuring the viability percentage with the 3'-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method and subsequently the IC50 of SAgNPs. Results: The presence of AgNPs was confirmed by silver surface plasmon resonance at 420 nm. The average size obtained by dynamic light scattering analysis was 98.96 nm, with a spherical and uniform shape according to transmission electron microscopy analysis. SAgNPs were tested at concentrations from 10 µg/mL to 0.3125 µg/mL and presented inhibition percentages from 3.76% to 90.09% with an IC50 value of 3.04 µg/mL (p-value of 0.02, 95% confidence level) in PANC-1 cells. Conclusions: The biologically synthesized nanoparticles with Stenocereus queretaroensis peel showed antiproliferative activity in PANC-1 pancreatic cancer cells. Therefore, these results suggest their potential use in the prevention and treatment of pancreatic cancer with further investigation.https://www.explorationpub.com/uploads/Article/A101328/101328.pdfsilver nanoparticlesbiogenic synthesisantiproliferative activitypancreatic cancerstenocereus queretaroensis |
| spellingShingle | Ariadna Abigail Villarreal-Amézquita Ivan Moises Sánchez-Hernández Eduardo Padilla-Camberos Silver nanoparticles biosynthesized from Stenocereus queretaroensis with antiproliferative activity Exploration of BioMat-X silver nanoparticles biogenic synthesis antiproliferative activity pancreatic cancer stenocereus queretaroensis |
| title | Silver nanoparticles biosynthesized from Stenocereus queretaroensis with antiproliferative activity |
| title_full | Silver nanoparticles biosynthesized from Stenocereus queretaroensis with antiproliferative activity |
| title_fullStr | Silver nanoparticles biosynthesized from Stenocereus queretaroensis with antiproliferative activity |
| title_full_unstemmed | Silver nanoparticles biosynthesized from Stenocereus queretaroensis with antiproliferative activity |
| title_short | Silver nanoparticles biosynthesized from Stenocereus queretaroensis with antiproliferative activity |
| title_sort | silver nanoparticles biosynthesized from stenocereus queretaroensis with antiproliferative activity |
| topic | silver nanoparticles biogenic synthesis antiproliferative activity pancreatic cancer stenocereus queretaroensis |
| url | https://www.explorationpub.com/uploads/Article/A101328/101328.pdf |
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