Silver nanoparticles biosynthesized from Stenocereus queretaroensis with antiproliferative activity
Aim: This work aimed to evaluate the antiproliferative activity of silver nanoparticles (AgNPs) biosynthesized with aqueous extract of Stenocereus queretaroensis peel (SAgNPs) in pancreatic ductal cancer cells PANC-1. Methods: Nanoparticles were synthesized using 2 mM silver nitrate (AgNO3) and a 1%...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Open Exploration Publishing Inc.
2025-02-01
|
| Series: | Exploration of BioMat-X |
| Subjects: | |
| Online Access: | https://www.explorationpub.com/uploads/Article/A101328/101328.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Aim: This work aimed to evaluate the antiproliferative activity of silver nanoparticles (AgNPs) biosynthesized with aqueous extract of Stenocereus queretaroensis peel (SAgNPs) in pancreatic ductal cancer cells PANC-1. Methods: Nanoparticles were synthesized using 2 mM silver nitrate (AgNO3) and a 1% aqueous extract of Stenocereus queretaroensis peel. SAgNPs were characterized by ultraviolet-visible spectroscopy (UV-Vis) light spectroscopy, dynamic light scattering analysis, and transmission electron microscopy. The antiproliferative activity was evaluated in the PANC-1 cell line by measuring the viability percentage with the 3'-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method and subsequently the IC50 of SAgNPs. Results: The presence of AgNPs was confirmed by silver surface plasmon resonance at 420 nm. The average size obtained by dynamic light scattering analysis was 98.96 nm, with a spherical and uniform shape according to transmission electron microscopy analysis. SAgNPs were tested at concentrations from 10 µg/mL to 0.3125 µg/mL and presented inhibition percentages from 3.76% to 90.09% with an IC50 value of 3.04 µg/mL (p-value of 0.02, 95% confidence level) in PANC-1 cells. Conclusions: The biologically synthesized nanoparticles with Stenocereus queretaroensis peel showed antiproliferative activity in PANC-1 pancreatic cancer cells. Therefore, these results suggest their potential use in the prevention and treatment of pancreatic cancer with further investigation. |
|---|---|
| ISSN: | 2996-9476 |