Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae
Background: Mitochondrial genomes play a key role in molecular biology research by providing essential information about evolutionary links, population history, and genetic diversity. Aim: The aim of this investigation was to produce partial sequence of 12S rRNA and 16S rRNA genes, as well as co...
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| Format: | Article |
| Language: | English |
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Tripoli University
2024-12-01
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| Series: | Open Veterinary Journal |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=217238 |
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| author | Najiah M. Alyamani |
| author_facet | Najiah M. Alyamani |
| author_sort | Najiah M. Alyamani |
| collection | DOAJ |
| description | Background:
Mitochondrial genomes play a key role in molecular biology research by providing essential information about evolutionary links, population history, and genetic diversity.
Aim:
The aim of this investigation was to produce partial sequence of 12S rRNA and 16S rRNA genes, as well as complete sequence of tRNA-Val gene in some species of family Labridae.
Methods:
Five species of labrid fishes (Oxycheilinus digramma, Cheilio inermis, Epibulus insidiator, Coris aygula and Gomphosus caeruleus) belong to Family Labridae were collected from the Red Sea, thereafter, taken to a lab for morphological identification in accordance with Randall (1982). Using forward and reverse primers (Wang, 2000), genome DNA was amplified through polymerase chain reaction.
Results:
The tRNA-Val gene's entire sequence, the 12S rRNA gene's partial sequence, and the 16S rRNA gene's partial sequence were all submitted to GenBank/NCBI with accession numbers (PP962382.1 - PP962386.1). The sequences' outcomes showed that the average A+T values were higher than the C+G values.
Conclusion:
The partial sequences of 12S RNA and 16S RNA, and the whole sequence of the tRNA-Val gene, were arranged that, the 12S RNA and 16S RNA have been distinguished by the tRNA-Val gene. [Open Vet J 2024; 14(12.000): 3336-3344] |
| format | Article |
| id | doaj-art-71dff98c7209446daf178ccaf1e72b9b |
| institution | DOAJ |
| issn | 2226-4485 2218-6050 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj-art-71dff98c7209446daf178ccaf1e72b9b2025-08-20T03:09:13ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502024-12-0114123336334410.5455/OVJ.2024.v14.i12.18217238Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family LabridaeNajiah M. Alyamani0Department of Biology, College of Science, University of Jeddah, Jeddah, Saudi ArabiaBackground: Mitochondrial genomes play a key role in molecular biology research by providing essential information about evolutionary links, population history, and genetic diversity. Aim: The aim of this investigation was to produce partial sequence of 12S rRNA and 16S rRNA genes, as well as complete sequence of tRNA-Val gene in some species of family Labridae. Methods: Five species of labrid fishes (Oxycheilinus digramma, Cheilio inermis, Epibulus insidiator, Coris aygula and Gomphosus caeruleus) belong to Family Labridae were collected from the Red Sea, thereafter, taken to a lab for morphological identification in accordance with Randall (1982). Using forward and reverse primers (Wang, 2000), genome DNA was amplified through polymerase chain reaction. Results: The tRNA-Val gene's entire sequence, the 12S rRNA gene's partial sequence, and the 16S rRNA gene's partial sequence were all submitted to GenBank/NCBI with accession numbers (PP962382.1 - PP962386.1). The sequences' outcomes showed that the average A+T values were higher than the C+G values. Conclusion: The partial sequences of 12S RNA and 16S RNA, and the whole sequence of the tRNA-Val gene, were arranged that, the 12S RNA and 16S RNA have been distinguished by the tRNA-Val gene. [Open Vet J 2024; 14(12.000): 3336-3344]http://www.ejmanager.com/fulltextpdf.php?mno=217238mitochondrial 12s rrna gene16s rrna genetrna-val genelabridae |
| spellingShingle | Najiah M. Alyamani Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae Open Veterinary Journal mitochondrial 12s rrna gene 16s rrna gene trna-val gene labridae |
| title | Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae |
| title_full | Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae |
| title_fullStr | Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae |
| title_full_unstemmed | Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae |
| title_short | Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae |
| title_sort | partial and complete sequence of small and large subunit ribosomal rna genes trna val gene in some species of family labridae |
| topic | mitochondrial 12s rrna gene 16s rrna gene trna-val gene labridae |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=217238 |
| work_keys_str_mv | AT najiahmalyamani partialandcompletesequenceofsmallandlargesubunitribosomalrnagenestrnavalgeneinsomespeciesoffamilylabridae |