Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae
Background: Mitochondrial genomes play a key role in molecular biology research by providing essential information about evolutionary links, population history, and genetic diversity. Aim: The aim of this investigation was to produce partial sequence of 12S rRNA and 16S rRNA genes, as well as co...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Tripoli University
2024-12-01
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| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=217238 |
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| Summary: | Background:
Mitochondrial genomes play a key role in molecular biology research by providing essential information about evolutionary links, population history, and genetic diversity.
Aim:
The aim of this investigation was to produce partial sequence of 12S rRNA and 16S rRNA genes, as well as complete sequence of tRNA-Val gene in some species of family Labridae.
Methods:
Five species of labrid fishes (Oxycheilinus digramma, Cheilio inermis, Epibulus insidiator, Coris aygula and Gomphosus caeruleus) belong to Family Labridae were collected from the Red Sea, thereafter, taken to a lab for morphological identification in accordance with Randall (1982). Using forward and reverse primers (Wang, 2000), genome DNA was amplified through polymerase chain reaction.
Results:
The tRNA-Val gene's entire sequence, the 12S rRNA gene's partial sequence, and the 16S rRNA gene's partial sequence were all submitted to GenBank/NCBI with accession numbers (PP962382.1 - PP962386.1). The sequences' outcomes showed that the average A+T values were higher than the C+G values.
Conclusion:
The partial sequences of 12S RNA and 16S RNA, and the whole sequence of the tRNA-Val gene, were arranged that, the 12S RNA and 16S RNA have been distinguished by the tRNA-Val gene. [Open Vet J 2024; 14(12.000): 3336-3344] |
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| ISSN: | 2226-4485 2218-6050 |