Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations
A competitive CZE method for quality control analysis of multivitamin preparations and veterinary products containing B-group vitamins was developed. Vitamins of interest are thiamine hydrochloride (B1), thiamine monophosphate chloride (B1a), riboflavine (B2), riboflavine-5′monophosphate (B2a), ni...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/592650 |
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| author | Marina Franco Renata Jasionowska Elisa Salvatore |
| author_facet | Marina Franco Renata Jasionowska Elisa Salvatore |
| author_sort | Marina Franco |
| collection | DOAJ |
| description | A competitive CZE method for quality control analysis of multivitamin preparations and veterinary products containing B-group vitamins was developed. Vitamins of interest are thiamine hydrochloride (B1), thiamine monophosphate chloride (B1a), riboflavine (B2), riboflavine-5′monophosphate (B2a), nicotinamide (B3), d-pantothenic acid calcium salt (B5), pyridoxine hydrochloride (B6), folic acid (B9), and 4-aminobenzoic acid (B10). These analytes were separated optimizing the experimental conditions in 20 mM tetraborate buffer pH=9.2 as a BGE (background electrolyte), on a Beckman P/ACE System MDQ instrument, using uncoated fused silica capillary.
The effective capillary length was of 49.5 cm, I.D.=50 μm, the applied voltage 20 kV and the temperature 25∘C. Detection was performed by a diode array detector at 214 nm for all vitamins except B5 (190 nm) and B2a (260 nm). Separation time was about 9 min. After experimental conditions optimization, the proposed method was validated. Precision of migration time and corrected peak area, linearity range, LOD and LOQ, accuracy (recovery), robustness, and ruggedness were evaluated for each analyte demonstrating the good reliability of the method. Analyses of the pharmaceutical real samples were performed and confirmed the versatility of this method. |
| format | Article |
| id | doaj-art-71bd99ddd2504c8ebc8e59e2b2d13712 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-71bd99ddd2504c8ebc8e59e2b2d137122025-02-03T01:23:54ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792012-01-01201210.1155/2012/592650592650Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary PreparationsMarina Franco0Renata Jasionowska1Elisa Salvatore2Dipartimento di Chimica, Sapienza Università, 00185 Roma, ItalyDipartimento di Chimica, Sapienza Università, 00185 Roma, ItalyDipartimento di Chimica, Sapienza Università, 00185 Roma, ItalyA competitive CZE method for quality control analysis of multivitamin preparations and veterinary products containing B-group vitamins was developed. Vitamins of interest are thiamine hydrochloride (B1), thiamine monophosphate chloride (B1a), riboflavine (B2), riboflavine-5′monophosphate (B2a), nicotinamide (B3), d-pantothenic acid calcium salt (B5), pyridoxine hydrochloride (B6), folic acid (B9), and 4-aminobenzoic acid (B10). These analytes were separated optimizing the experimental conditions in 20 mM tetraborate buffer pH=9.2 as a BGE (background electrolyte), on a Beckman P/ACE System MDQ instrument, using uncoated fused silica capillary. The effective capillary length was of 49.5 cm, I.D.=50 μm, the applied voltage 20 kV and the temperature 25∘C. Detection was performed by a diode array detector at 214 nm for all vitamins except B5 (190 nm) and B2a (260 nm). Separation time was about 9 min. After experimental conditions optimization, the proposed method was validated. Precision of migration time and corrected peak area, linearity range, LOD and LOQ, accuracy (recovery), robustness, and ruggedness were evaluated for each analyte demonstrating the good reliability of the method. Analyses of the pharmaceutical real samples were performed and confirmed the versatility of this method.http://dx.doi.org/10.1155/2012/592650 |
| spellingShingle | Marina Franco Renata Jasionowska Elisa Salvatore Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations International Journal of Analytical Chemistry |
| title | Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations |
| title_full | Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations |
| title_fullStr | Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations |
| title_full_unstemmed | Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations |
| title_short | Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations |
| title_sort | application of cze method in routine analysis for determination of b complex vitamins in pharmaceutical and veterinary preparations |
| url | http://dx.doi.org/10.1155/2012/592650 |
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