Potential Antioxidant Compounds from the Spores of <i>Dicranopteris linearis</i> and the Branches of <i>Averrhoa bilimbi</i>
This study focused on bio-guided isolation based on antioxidant activities from <i>Dicranopteris linearis</i> spores and <i>Averrhoa bilimbi</i> branches. The total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities of the extracts were determin...
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2024-10-01
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| author | Thuc-Huy Duong Thi-Minh-Dinh Tran Phuong-Mai To Nguyen-Hong-Nhi Phan Thi-Phuong Nguyen Huong Thuy Le Jirapast Sichaem |
| author_facet | Thuc-Huy Duong Thi-Minh-Dinh Tran Phuong-Mai To Nguyen-Hong-Nhi Phan Thi-Phuong Nguyen Huong Thuy Le Jirapast Sichaem |
| author_sort | Thuc-Huy Duong |
| collection | DOAJ |
| description | This study focused on bio-guided isolation based on antioxidant activities from <i>Dicranopteris linearis</i> spores and <i>Averrhoa bilimbi</i> branches. The total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities of the extracts were determined. For <i>D. linearis</i> spores, the ethyl acetate (EA) extract exhibited the highest TPC (120.13 ± 0.04 mg GAE/g) and TFC (21.94 ± 0.30 mg QE/g), along with strong DPPH antioxidant activity (96.3 ± 0.3% inhibition, IC<sub>50</sub> of 39.4 ± 0.3 µg/mL). For <i>A. bilimbi</i> branches, the <i>n</i>-hexane–ethyl acetate (HEA) extract showed the highest TPC (165.21 ± 0.24 mg GAE/g) and TFC (26.20 ± 0.01 mg QE/g), with significant DPPH antioxidant activity (89.6 ± 0.7% inhibition, IC<sub>50</sub> of 39.7 ± 1.9 µg/mL). Phytochemical investigation led to the identification of ten compounds (<b>D1</b>–<b>D10</b>) from <i>D. linearis</i> spores and twelve compounds (<b>A1</b>–<b>A12</b>) from <i>A. bilimbi</i> branches. Notably, compound <b>A1</b> was identified as a new natural compound. The chemical structures were elucidated through NMR spectroscopy and comparison with existing literature. The antioxidant activities of selected compounds (<b>D3</b>–<b>D5</b>, <b>D8</b>–<b>D10</b>, and <b>A1</b>–<b>A11</b>) were evaluated using DPPH and ABTS free radical scavenging assays. Among them, compound <b>A3</b> exhibited the strongest antioxidant activities (IC<sub>50</sub> of 7.1 ± 0.1 µg/mL for DPPH and 14.8 ± 0.1 for ABTS, respectively). The results of this study highlight the potential of <i>D. linearis</i> and <i>A. bilimbi</i> for use in natural product-based antioxidant applications. |
| format | Article |
| id | doaj-art-71bb8a4577514ed99827664944ce8943 |
| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-10-01 |
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| spelling | doaj-art-71bb8a4577514ed99827664944ce89432025-08-20T02:26:52ZengMDPI AGAntioxidants2076-39212024-10-011311131910.3390/antiox13111319Potential Antioxidant Compounds from the Spores of <i>Dicranopteris linearis</i> and the Branches of <i>Averrhoa bilimbi</i>Thuc-Huy Duong0Thi-Minh-Dinh Tran1Phuong-Mai To2Nguyen-Hong-Nhi Phan3Thi-Phuong Nguyen4Huong Thuy Le5Jirapast Sichaem6Department of Chemistry, Ho Chi Minh City University of Education, 280 An Duong Vuong Street, District 5, Ho Chi Minh City 700000, VietnamDepartment of Biology, Ho Chi Minh City University of Education, 280 An Duong Vuong Street, District 5, Ho Chi Minh City 700000, VietnamDepartment of Chemistry, Ho Chi Minh City University of Education, 280 An Duong Vuong Street, District 5, Ho Chi Minh City 700000, VietnamNTT Hi-Tech Institute, Nguyen Tat Thanh University, 300A Nguyen Tat Thanh, District 4, Ho Chi Minh City 700000, VietnamNTT Hi-Tech Institute, Nguyen Tat Thanh University, 300A Nguyen Tat Thanh, District 4, Ho Chi Minh City 700000, VietnamFaculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, VietnamResearch Unit in Natural Products Chemistry and Bioactivities, Faculty of Science and Technology, Thammasat University Lampang Campus, Lampang 52190, ThailandThis study focused on bio-guided isolation based on antioxidant activities from <i>Dicranopteris linearis</i> spores and <i>Averrhoa bilimbi</i> branches. The total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities of the extracts were determined. For <i>D. linearis</i> spores, the ethyl acetate (EA) extract exhibited the highest TPC (120.13 ± 0.04 mg GAE/g) and TFC (21.94 ± 0.30 mg QE/g), along with strong DPPH antioxidant activity (96.3 ± 0.3% inhibition, IC<sub>50</sub> of 39.4 ± 0.3 µg/mL). For <i>A. bilimbi</i> branches, the <i>n</i>-hexane–ethyl acetate (HEA) extract showed the highest TPC (165.21 ± 0.24 mg GAE/g) and TFC (26.20 ± 0.01 mg QE/g), with significant DPPH antioxidant activity (89.6 ± 0.7% inhibition, IC<sub>50</sub> of 39.7 ± 1.9 µg/mL). Phytochemical investigation led to the identification of ten compounds (<b>D1</b>–<b>D10</b>) from <i>D. linearis</i> spores and twelve compounds (<b>A1</b>–<b>A12</b>) from <i>A. bilimbi</i> branches. Notably, compound <b>A1</b> was identified as a new natural compound. The chemical structures were elucidated through NMR spectroscopy and comparison with existing literature. The antioxidant activities of selected compounds (<b>D3</b>–<b>D5</b>, <b>D8</b>–<b>D10</b>, and <b>A1</b>–<b>A11</b>) were evaluated using DPPH and ABTS free radical scavenging assays. Among them, compound <b>A3</b> exhibited the strongest antioxidant activities (IC<sub>50</sub> of 7.1 ± 0.1 µg/mL for DPPH and 14.8 ± 0.1 for ABTS, respectively). The results of this study highlight the potential of <i>D. linearis</i> and <i>A. bilimbi</i> for use in natural product-based antioxidant applications.https://www.mdpi.com/2076-3921/13/11/1319<i>Dicranopteris linearis</i><i>Averrhoa bilimbi</i>bio-guided isolationantioxidant activityphenolic contentflavonoid content |
| spellingShingle | Thuc-Huy Duong Thi-Minh-Dinh Tran Phuong-Mai To Nguyen-Hong-Nhi Phan Thi-Phuong Nguyen Huong Thuy Le Jirapast Sichaem Potential Antioxidant Compounds from the Spores of <i>Dicranopteris linearis</i> and the Branches of <i>Averrhoa bilimbi</i> Antioxidants <i>Dicranopteris linearis</i> <i>Averrhoa bilimbi</i> bio-guided isolation antioxidant activity phenolic content flavonoid content |
| title | Potential Antioxidant Compounds from the Spores of <i>Dicranopteris linearis</i> and the Branches of <i>Averrhoa bilimbi</i> |
| title_full | Potential Antioxidant Compounds from the Spores of <i>Dicranopteris linearis</i> and the Branches of <i>Averrhoa bilimbi</i> |
| title_fullStr | Potential Antioxidant Compounds from the Spores of <i>Dicranopteris linearis</i> and the Branches of <i>Averrhoa bilimbi</i> |
| title_full_unstemmed | Potential Antioxidant Compounds from the Spores of <i>Dicranopteris linearis</i> and the Branches of <i>Averrhoa bilimbi</i> |
| title_short | Potential Antioxidant Compounds from the Spores of <i>Dicranopteris linearis</i> and the Branches of <i>Averrhoa bilimbi</i> |
| title_sort | potential antioxidant compounds from the spores of i dicranopteris linearis i and the branches of i averrhoa bilimbi i |
| topic | <i>Dicranopteris linearis</i> <i>Averrhoa bilimbi</i> bio-guided isolation antioxidant activity phenolic content flavonoid content |
| url | https://www.mdpi.com/2076-3921/13/11/1319 |
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