<i>cpt1b</i> Regulates Cardiomyocyte Proliferation Through Modulation of Glutamine Synthetase in Zebrafish
Carnitine palmitoyltransferase 1b (Cpt1b) is a crucial rate-limiting enzyme in fatty acid metabolism, but its role and mechanism in early cardiac development remains unclear. Here, we show that <i>cpt1b</i> regulates cardiomyocyte proliferation during zebrafish development. Knocking out...
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MDPI AG
2024-11-01
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| Series: | Journal of Cardiovascular Development and Disease |
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| Online Access: | https://www.mdpi.com/2308-3425/11/11/344 |
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| author | Xiaohan Cheng Jingyi Ju Wenping Huang Zongyi Duan Yanchao Han |
| author_facet | Xiaohan Cheng Jingyi Ju Wenping Huang Zongyi Duan Yanchao Han |
| author_sort | Xiaohan Cheng |
| collection | DOAJ |
| description | Carnitine palmitoyltransferase 1b (Cpt1b) is a crucial rate-limiting enzyme in fatty acid metabolism, but its role and mechanism in early cardiac development remains unclear. Here, we show that <i>cpt1b</i> regulates cardiomyocyte proliferation during zebrafish development. Knocking out entire <i>cpt1b</i> coding sequences leads to impaired cardiomyocyte proliferation, while cardiomyocyte-specific overexpression of <i>cpt1b</i> promotes cardiomyocyte proliferation. RNA sequencing analysis and pharmacological studies identified glutamine synthetase as a key downstream effector of <i>cpt1b</i> in regulating cardiomyocyte proliferation. Our study elucidates a novel mechanism whereby <i>cpt1b</i> promotes zebrafish cardiomyocyte proliferation through glutamine synthetase, which provides new perspectives on the significance of fatty acid metabolism in heart development and the interplay between fatty acid and amino acid metabolic pathways. |
| format | Article |
| id | doaj-art-71b69bc4ca6d439985bb654a17489f09 |
| institution | OA Journals |
| issn | 2308-3425 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Cardiovascular Development and Disease |
| spelling | doaj-art-71b69bc4ca6d439985bb654a17489f092025-08-20T02:04:52ZengMDPI AGJournal of Cardiovascular Development and Disease2308-34252024-11-01111134410.3390/jcdd11110344<i>cpt1b</i> Regulates Cardiomyocyte Proliferation Through Modulation of Glutamine Synthetase in ZebrafishXiaohan Cheng0Jingyi Ju1Wenping Huang2Zongyi Duan3Yanchao Han4Institute for Cardiovascular Science and Department of Cardiovascular Surgery of the First Affiliated Hospital of Soochow University, Suzhou Medical College, Soochow University, Suzhou 215000, ChinaInstitute for Cardiovascular Science and Department of Cardiovascular Surgery of the First Affiliated Hospital of Soochow University, Suzhou Medical College, Soochow University, Suzhou 215000, ChinaInstitute for Cardiovascular Science and Department of Cardiovascular Surgery of the First Affiliated Hospital of Soochow University, Suzhou Medical College, Soochow University, Suzhou 215000, ChinaInstitute for Cardiovascular Science and Department of Cardiovascular Surgery of the First Affiliated Hospital of Soochow University, Suzhou Medical College, Soochow University, Suzhou 215000, ChinaInstitute for Cardiovascular Science and Department of Cardiovascular Surgery of the First Affiliated Hospital of Soochow University, Suzhou Medical College, Soochow University, Suzhou 215000, ChinaCarnitine palmitoyltransferase 1b (Cpt1b) is a crucial rate-limiting enzyme in fatty acid metabolism, but its role and mechanism in early cardiac development remains unclear. Here, we show that <i>cpt1b</i> regulates cardiomyocyte proliferation during zebrafish development. Knocking out entire <i>cpt1b</i> coding sequences leads to impaired cardiomyocyte proliferation, while cardiomyocyte-specific overexpression of <i>cpt1b</i> promotes cardiomyocyte proliferation. RNA sequencing analysis and pharmacological studies identified glutamine synthetase as a key downstream effector of <i>cpt1b</i> in regulating cardiomyocyte proliferation. Our study elucidates a novel mechanism whereby <i>cpt1b</i> promotes zebrafish cardiomyocyte proliferation through glutamine synthetase, which provides new perspectives on the significance of fatty acid metabolism in heart development and the interplay between fatty acid and amino acid metabolic pathways.https://www.mdpi.com/2308-3425/11/11/344cardiomyocyte proliferation<i>cpt1b</i><i>glul</i>metabolismzebrafish |
| spellingShingle | Xiaohan Cheng Jingyi Ju Wenping Huang Zongyi Duan Yanchao Han <i>cpt1b</i> Regulates Cardiomyocyte Proliferation Through Modulation of Glutamine Synthetase in Zebrafish Journal of Cardiovascular Development and Disease cardiomyocyte proliferation <i>cpt1b</i> <i>glul</i> metabolism zebrafish |
| title | <i>cpt1b</i> Regulates Cardiomyocyte Proliferation Through Modulation of Glutamine Synthetase in Zebrafish |
| title_full | <i>cpt1b</i> Regulates Cardiomyocyte Proliferation Through Modulation of Glutamine Synthetase in Zebrafish |
| title_fullStr | <i>cpt1b</i> Regulates Cardiomyocyte Proliferation Through Modulation of Glutamine Synthetase in Zebrafish |
| title_full_unstemmed | <i>cpt1b</i> Regulates Cardiomyocyte Proliferation Through Modulation of Glutamine Synthetase in Zebrafish |
| title_short | <i>cpt1b</i> Regulates Cardiomyocyte Proliferation Through Modulation of Glutamine Synthetase in Zebrafish |
| title_sort | i cpt1b i regulates cardiomyocyte proliferation through modulation of glutamine synthetase in zebrafish |
| topic | cardiomyocyte proliferation <i>cpt1b</i> <i>glul</i> metabolism zebrafish |
| url | https://www.mdpi.com/2308-3425/11/11/344 |
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