The A-C linker controls centriole structural integrity and duplication
Abstract Centrioles are evolutionarily conserved barrel-shaped organelles playing crucial roles in cell division and ciliogenesis. These functions are underpinned by specific structural sub-elements whose functions have been under investigation since many years. The A-C linker structure, connecting...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-62154-6 |
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| author | Lorène Bournonville Marine. H. Laporte Susanne Borgers Paul Guichard Virginie Hamel |
| author_facet | Lorène Bournonville Marine. H. Laporte Susanne Borgers Paul Guichard Virginie Hamel |
| author_sort | Lorène Bournonville |
| collection | DOAJ |
| description | Abstract Centrioles are evolutionarily conserved barrel-shaped organelles playing crucial roles in cell division and ciliogenesis. These functions are underpinned by specific structural sub-elements whose functions have been under investigation since many years. The A-C linker structure, connecting adjacent microtubule triplets in the proximal region, has remained unexplored due to its unknown composition. Here, using ultrastructure expansion microscopy, we characterized two recently identified A-C linker proteins, CCDC77 and WDR67, and discovered MIIP as an additional A-C linker protein. Our findings reveal that these proteins localize between microtubule triplets at the A-C linker, forming a complex. Depletion of A-C linker components disrupt microtubule triplet cohesion, leading to breakage at the proximal end. Co-removal of the A-C linker and the inner scaffold demonstrates their joint role in maintaining centriole architecture. Moreover, we uncover an unexpected function of the A-C linker in centriole duplication through torus regulation, underscoring the interplay between these protein modules. |
| format | Article |
| id | doaj-art-71b66d89dc6f431db482954868a83d76 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-71b66d89dc6f431db482954868a83d762025-08-20T04:02:55ZengNature PortfolioNature Communications2041-17232025-07-0116111910.1038/s41467-025-62154-6The A-C linker controls centriole structural integrity and duplicationLorène Bournonville0Marine. H. Laporte1Susanne Borgers2Paul Guichard3Virginie Hamel4University of Geneva, Faculty of Sciences, Department of Molecular and Cellular BiologyUniversity of Geneva, Faculty of Sciences, Department of Molecular and Cellular BiologyUniversity of Geneva, Faculty of Sciences, Department of Molecular and Cellular BiologyUniversity of Geneva, Faculty of Sciences, Department of Molecular and Cellular BiologyUniversity of Geneva, Faculty of Sciences, Department of Molecular and Cellular BiologyAbstract Centrioles are evolutionarily conserved barrel-shaped organelles playing crucial roles in cell division and ciliogenesis. These functions are underpinned by specific structural sub-elements whose functions have been under investigation since many years. The A-C linker structure, connecting adjacent microtubule triplets in the proximal region, has remained unexplored due to its unknown composition. Here, using ultrastructure expansion microscopy, we characterized two recently identified A-C linker proteins, CCDC77 and WDR67, and discovered MIIP as an additional A-C linker protein. Our findings reveal that these proteins localize between microtubule triplets at the A-C linker, forming a complex. Depletion of A-C linker components disrupt microtubule triplet cohesion, leading to breakage at the proximal end. Co-removal of the A-C linker and the inner scaffold demonstrates their joint role in maintaining centriole architecture. Moreover, we uncover an unexpected function of the A-C linker in centriole duplication through torus regulation, underscoring the interplay between these protein modules.https://doi.org/10.1038/s41467-025-62154-6 |
| spellingShingle | Lorène Bournonville Marine. H. Laporte Susanne Borgers Paul Guichard Virginie Hamel The A-C linker controls centriole structural integrity and duplication Nature Communications |
| title | The A-C linker controls centriole structural integrity and duplication |
| title_full | The A-C linker controls centriole structural integrity and duplication |
| title_fullStr | The A-C linker controls centriole structural integrity and duplication |
| title_full_unstemmed | The A-C linker controls centriole structural integrity and duplication |
| title_short | The A-C linker controls centriole structural integrity and duplication |
| title_sort | a c linker controls centriole structural integrity and duplication |
| url | https://doi.org/10.1038/s41467-025-62154-6 |
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