A temperature responsive hydrogel encapsulated with adipose‐derived stem cells and melanin promotes repair and regeneration of endometrial injury
Abstract The endometrium, the inner lining of the uterus, assumes a crucial role in the female reproductive system. Disorders and injuries impacting the endometrium can lead to profound consequences, including infertility and compromised women's overall health. Recent advancements in stem cell...
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Wiley
2025-01-01
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| Series: | Bioengineering & Translational Medicine |
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| Online Access: | https://doi.org/10.1002/btm2.10714 |
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| author | Ruigao Song Chicheng Ma Hongxia Li Yu Cheng Xianmei Cui Zanhong Wang Lijuan Huang Chunying Song Yukai Jing Bing Cao Lili Wang Qing Tian Xi Wang Ruiping Zhang Hanwang Zhang |
| author_facet | Ruigao Song Chicheng Ma Hongxia Li Yu Cheng Xianmei Cui Zanhong Wang Lijuan Huang Chunying Song Yukai Jing Bing Cao Lili Wang Qing Tian Xi Wang Ruiping Zhang Hanwang Zhang |
| author_sort | Ruigao Song |
| collection | DOAJ |
| description | Abstract The endometrium, the inner lining of the uterus, assumes a crucial role in the female reproductive system. Disorders and injuries impacting the endometrium can lead to profound consequences, including infertility and compromised women's overall health. Recent advancements in stem cell research have opened new possibilities for the treatment and repair of endometrial issues. In the present study, we constructed a degradable hydrogel by loading adipose‐derived stem cells (ADSCs) and melanin nanoparticles (MNP). In vitro cell experiments validated the biocompatibility of the prepared hydrogels and their adeptness in encapsulating ADSCs. Subsequently, we explored the impact of hydrogel@ADSC@MNP constructs in the healing process of uterine injury in mice. The results indicated that hydrogel@ADSC@MNP could augment endometrial thickness and ameliorate endometrial interstitial fibrosis. The injured tissue adjacent to hydrogel@ADSC@MNP constructs exhibited higher levels of bFGF, IGF‐1, and VEGFA compared with the corresponding tissue in mice receiving hydrogel constructs alone or in the model group. Furthermore, the hydrogel@ADSC@MNP system enhanced the proliferative capabilities of uterine endometrial cells, facilitated microvasculature regeneration, and reinstated the endometrium's capacity to receive the embryos. Our findings strongly suggest that the hydrogel@ADSC@MNP system holds significant promise for repairing and regenerating damaged endometrium. |
| format | Article |
| id | doaj-art-71ab012248b54e238778bc8e6f300538 |
| institution | Kabale University |
| issn | 2380-6761 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj-art-71ab012248b54e238778bc8e6f3005382025-01-09T06:19:46ZengWileyBioengineering & Translational Medicine2380-67612025-01-01101n/an/a10.1002/btm2.10714A temperature responsive hydrogel encapsulated with adipose‐derived stem cells and melanin promotes repair and regeneration of endometrial injuryRuigao Song0Chicheng Ma1Hongxia Li2Yu Cheng3Xianmei Cui4Zanhong Wang5Lijuan Huang6Chunying Song7Yukai Jing8Bing Cao9Lili Wang10Qing Tian11Xi Wang12Ruiping Zhang13Hanwang Zhang14Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital Taiyuan ChinaCollege of Animal Science, Shanxi Agricultural University Taigu ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital Taiyuan ChinaCollege of Animal Science, Shanxi Agricultural University Taigu ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital Taiyuan ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital Taiyuan ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital Taiyuan ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital Taiyuan ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital Taiyuan ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital Taiyuan ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital Taiyuan ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital Taiyuan ChinaCollege of Animal Science, Shanxi Agricultural University Taigu ChinaThe Radiology Department of Shanxi Provincial People's Hospital The Fifth Hospital of Shanxi Medical University Taiyuan ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital Taiyuan ChinaAbstract The endometrium, the inner lining of the uterus, assumes a crucial role in the female reproductive system. Disorders and injuries impacting the endometrium can lead to profound consequences, including infertility and compromised women's overall health. Recent advancements in stem cell research have opened new possibilities for the treatment and repair of endometrial issues. In the present study, we constructed a degradable hydrogel by loading adipose‐derived stem cells (ADSCs) and melanin nanoparticles (MNP). In vitro cell experiments validated the biocompatibility of the prepared hydrogels and their adeptness in encapsulating ADSCs. Subsequently, we explored the impact of hydrogel@ADSC@MNP constructs in the healing process of uterine injury in mice. The results indicated that hydrogel@ADSC@MNP could augment endometrial thickness and ameliorate endometrial interstitial fibrosis. The injured tissue adjacent to hydrogel@ADSC@MNP constructs exhibited higher levels of bFGF, IGF‐1, and VEGFA compared with the corresponding tissue in mice receiving hydrogel constructs alone or in the model group. Furthermore, the hydrogel@ADSC@MNP system enhanced the proliferative capabilities of uterine endometrial cells, facilitated microvasculature regeneration, and reinstated the endometrium's capacity to receive the embryos. Our findings strongly suggest that the hydrogel@ADSC@MNP system holds significant promise for repairing and regenerating damaged endometrium.https://doi.org/10.1002/btm2.10714ADSCsendometriumhydrogelMNPregeneration |
| spellingShingle | Ruigao Song Chicheng Ma Hongxia Li Yu Cheng Xianmei Cui Zanhong Wang Lijuan Huang Chunying Song Yukai Jing Bing Cao Lili Wang Qing Tian Xi Wang Ruiping Zhang Hanwang Zhang A temperature responsive hydrogel encapsulated with adipose‐derived stem cells and melanin promotes repair and regeneration of endometrial injury Bioengineering & Translational Medicine ADSCs endometrium hydrogel MNP regeneration |
| title | A temperature responsive hydrogel encapsulated with adipose‐derived stem cells and melanin promotes repair and regeneration of endometrial injury |
| title_full | A temperature responsive hydrogel encapsulated with adipose‐derived stem cells and melanin promotes repair and regeneration of endometrial injury |
| title_fullStr | A temperature responsive hydrogel encapsulated with adipose‐derived stem cells and melanin promotes repair and regeneration of endometrial injury |
| title_full_unstemmed | A temperature responsive hydrogel encapsulated with adipose‐derived stem cells and melanin promotes repair and regeneration of endometrial injury |
| title_short | A temperature responsive hydrogel encapsulated with adipose‐derived stem cells and melanin promotes repair and regeneration of endometrial injury |
| title_sort | temperature responsive hydrogel encapsulated with adipose derived stem cells and melanin promotes repair and regeneration of endometrial injury |
| topic | ADSCs endometrium hydrogel MNP regeneration |
| url | https://doi.org/10.1002/btm2.10714 |
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