Culture and culture-independent diagnostic tests in Campylobacter enteritis
Introduction: Campylobacter infections are among the most common causes of bacterial enteritis. This study aims to determine the sensitivity, specificity and positive predictive values (PPV) of culture and culture-independent tests for the diagnosis of Campylobacter enteritis. Methodology: A tot...
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The Journal of Infection in Developing Countries
2022-04-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/14902 |
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| author | Nida Özcan Fatma Bacalan Fatih Çakır Alican Bilden Neslihan Genişel Tuba Dal |
| author_facet | Nida Özcan Fatma Bacalan Fatih Çakır Alican Bilden Neslihan Genişel Tuba Dal |
| author_sort | Nida Özcan |
| collection | DOAJ |
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Introduction: Campylobacter infections are among the most common causes of bacterial enteritis. This study aims to determine the sensitivity, specificity and positive predictive values (PPV) of culture and culture-independent tests for the diagnosis of Campylobacter enteritis.
Methodology: A total of 400 stool samples were included in the study. BD MAX enteric bacterial panel (BD Diagnostics, Franklin Lakes, NJ, USA) and EntericBio Gastro Panel II (Serosep, Limerick, Ireland) were used as commercial molecular tests. RIDA®QUICK Campylobacter (R-Biopharm, Darmstadt,, Germany) and CerTest (Biotec, Zaragoza, Spain) were used to detect Campylobacter antigens. Samples were cultured in CCDA media and subjected to bacterial identification by mass spectrometry.
Results: Among the 400 specimens, 41 (10.2%) were evaluated as Campylobacter positive; 21 were culture-positive and 20 were detected as positive by both PCR methods. Of the 21 isolates grown in culture, 16 (76.2%) were identified as C. jejuni and 5 (23.8%) as C. coli. While all 21 culture-positive specimens were detected as positive by both molecular tests, 18 of the specimens were found positive by RidaQuick, and 16 by Certest ICA. Of the 20 culture-negative Campylobacter cases, 18 were positive by RidaQuick and 12 by Certest ICA. Sensitivities of culture, ICA-RidaQuick and ICA-CerTest were 51.2%, 87.8 and 68.3, respectively. The specificities of all tests were in the range of 90-100 %. PPV of molecular tests, ICA-RidaQuick and ICA-CerTest were > 95%, 72 % and 48.3 %, respectively.
Conclusions: Molecular tests were superior to culture and ICA in terms of sensitivity, specificity, and positive predictive value.
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| format | Article |
| id | doaj-art-71a6f8a5d4c2422c8c3f1a1df1b3726e |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2022-04-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-71a6f8a5d4c2422c8c3f1a1df1b3726e2025-08-20T02:14:11ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802022-04-01160410.3855/jidc.14902Culture and culture-independent diagnostic tests in Campylobacter enteritisNida Özcan0Fatma Bacalan1Fatih Çakır2Alican Bilden3Neslihan Genişel4Tuba Dal5Dicle University Medicine Faculty, Medical Microbiology Department, Diyarbakır, TurkeyDiyarbakır Children’s Hospital, Laboratory of Microbiology, Diyarbakır, TurkeyDiyarbakır Children’s Hospital, Laboratory of Microbiology, Diyarbakır, TurkeyDicle University Medicine Faculty, Medical Microbiology Department, Diyarbakır, TurkeyDicle University Pharmacy Faculty, Diyarbakır, TurkeyYıldırım Beyazıt University Medicine Faculty, Ankara, Turkey Introduction: Campylobacter infections are among the most common causes of bacterial enteritis. This study aims to determine the sensitivity, specificity and positive predictive values (PPV) of culture and culture-independent tests for the diagnosis of Campylobacter enteritis. Methodology: A total of 400 stool samples were included in the study. BD MAX enteric bacterial panel (BD Diagnostics, Franklin Lakes, NJ, USA) and EntericBio Gastro Panel II (Serosep, Limerick, Ireland) were used as commercial molecular tests. RIDA®QUICK Campylobacter (R-Biopharm, Darmstadt,, Germany) and CerTest (Biotec, Zaragoza, Spain) were used to detect Campylobacter antigens. Samples were cultured in CCDA media and subjected to bacterial identification by mass spectrometry. Results: Among the 400 specimens, 41 (10.2%) were evaluated as Campylobacter positive; 21 were culture-positive and 20 were detected as positive by both PCR methods. Of the 21 isolates grown in culture, 16 (76.2%) were identified as C. jejuni and 5 (23.8%) as C. coli. While all 21 culture-positive specimens were detected as positive by both molecular tests, 18 of the specimens were found positive by RidaQuick, and 16 by Certest ICA. Of the 20 culture-negative Campylobacter cases, 18 were positive by RidaQuick and 12 by Certest ICA. Sensitivities of culture, ICA-RidaQuick and ICA-CerTest were 51.2%, 87.8 and 68.3, respectively. The specificities of all tests were in the range of 90-100 %. PPV of molecular tests, ICA-RidaQuick and ICA-CerTest were > 95%, 72 % and 48.3 %, respectively. Conclusions: Molecular tests were superior to culture and ICA in terms of sensitivity, specificity, and positive predictive value. https://jidc.org/index.php/journal/article/view/14902Campylobactergastroenteritismultiplex PCRimmunochromatography |
| spellingShingle | Nida Özcan Fatma Bacalan Fatih Çakır Alican Bilden Neslihan Genişel Tuba Dal Culture and culture-independent diagnostic tests in Campylobacter enteritis Journal of Infection in Developing Countries Campylobacter gastroenteritis multiplex PCR immunochromatography |
| title | Culture and culture-independent diagnostic tests in Campylobacter enteritis |
| title_full | Culture and culture-independent diagnostic tests in Campylobacter enteritis |
| title_fullStr | Culture and culture-independent diagnostic tests in Campylobacter enteritis |
| title_full_unstemmed | Culture and culture-independent diagnostic tests in Campylobacter enteritis |
| title_short | Culture and culture-independent diagnostic tests in Campylobacter enteritis |
| title_sort | culture and culture independent diagnostic tests in campylobacter enteritis |
| topic | Campylobacter gastroenteritis multiplex PCR immunochromatography |
| url | https://jidc.org/index.php/journal/article/view/14902 |
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