The careful selection of zwitterionic nanoparticle coating results in rapid and efficient cell labeling for imaging‐based cell tracking
Abstract The increased clinical application of cell‐based therapies has resulted in a parallel increase in the need for non‐invasive imaging‐based approaches for cell tracking, often through labeling with nanoparticles. An ideal nanoparticle for such applications must be biologically compatible as w...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-12-01
|
| Series: | Aggregate |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/agt2.609 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850064972790366208 |
|---|---|
| author | Nicholas D. Calvert Luciana Yu Olivia C. Sehl Julia J. Gevaert Natasha N. Knier Angelie Rivera‐Rodriguez Clara S. Goulet Nitara Fernando Samantha Flood Carlos M. Rinaldi‐Ramos Paula J. Foster Adam J. Shuhendler |
| author_facet | Nicholas D. Calvert Luciana Yu Olivia C. Sehl Julia J. Gevaert Natasha N. Knier Angelie Rivera‐Rodriguez Clara S. Goulet Nitara Fernando Samantha Flood Carlos M. Rinaldi‐Ramos Paula J. Foster Adam J. Shuhendler |
| author_sort | Nicholas D. Calvert |
| collection | DOAJ |
| description | Abstract The increased clinical application of cell‐based therapies has resulted in a parallel increase in the need for non‐invasive imaging‐based approaches for cell tracking, often through labeling with nanoparticles. An ideal nanoparticle for such applications must be biologically compatible as well as readily internalized by cells to ensure adequate and stable cell loading. Surface coatings have been used to make nanoparticle trackers suitable for these purposes, but those currently employed tend to have cytotoxic effects. Zwitterionic ligands are known to be biocompatible and antifouling; however, head‐to‐head evaluation of specific zwitterionic ligands for cell loading has not yet been explored. Magnetic particle imaging (MPI) detects superparamagnetic iron oxide nanoparticles (SPIONs) using time‐varying magnetic fields. Because MPI can produce high‐contrast, real‐time images with no tissue depth limitation, it is an ideal candidate for in vivo cell tracking. In this work, we have conjugated hard (permanently charged) and soft (pKa‐dependently charged) biomimetic zwitterionic ligands to SPIONs and characterized how these ligands changed SPION physicochemical properties. We have evaluated cellular uptake and subcellular localization between zwitterions, how the improvement in cell uptake generated stronger MPI signal for smaller numbers of cells, and how these cells can be tracked in an animal model with greater sensitivity for longer periods of time. Our best‐performing surface coating afforded high cell loading within 4 h, with full signal retention in vivo over 7 days. |
| format | Article |
| id | doaj-art-71a5e0d35fb94fecae4472548b319b2c |
| institution | DOAJ |
| issn | 2692-4560 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aggregate |
| spelling | doaj-art-71a5e0d35fb94fecae4472548b319b2c2025-08-20T02:49:08ZengWileyAggregate2692-45602024-12-0156n/an/a10.1002/agt2.609The careful selection of zwitterionic nanoparticle coating results in rapid and efficient cell labeling for imaging‐based cell trackingNicholas D. Calvert0Luciana Yu1Olivia C. Sehl2Julia J. Gevaert3Natasha N. Knier4Angelie Rivera‐Rodriguez5Clara S. Goulet6Nitara Fernando7Samantha Flood8Carlos M. Rinaldi‐Ramos9Paula J. Foster10Adam J. Shuhendler11Department of Chemistry and Biomolecular Sciences University of Ottawa Ottawa Ontario CanadaDepartment of Chemistry and Biomolecular Sciences University of Ottawa Ottawa Ontario CanadaDepartment of Medical Biophysics Western University London Ontario CanadaDepartment of Medical Biophysics Western University London Ontario CanadaDepartment of Medical Biophysics Western University London Ontario CanadaJ. Crayton Pruitt Family Department of Biomedical Engineering University of Florida Gainesville Florida USADepartment of Chemistry and Biomolecular Sciences University of Ottawa Ottawa Ontario CanadaDepartment of Medical Biophysics Western University London Ontario CanadaDepartment of Medical Biophysics Western University London Ontario CanadaJ. Crayton Pruitt Family Department of Biomedical Engineering University of Florida Gainesville Florida USADepartment of Medical Biophysics Western University London Ontario CanadaDepartment of Chemistry and Biomolecular Sciences University of Ottawa Ottawa Ontario CanadaAbstract The increased clinical application of cell‐based therapies has resulted in a parallel increase in the need for non‐invasive imaging‐based approaches for cell tracking, often through labeling with nanoparticles. An ideal nanoparticle for such applications must be biologically compatible as well as readily internalized by cells to ensure adequate and stable cell loading. Surface coatings have been used to make nanoparticle trackers suitable for these purposes, but those currently employed tend to have cytotoxic effects. Zwitterionic ligands are known to be biocompatible and antifouling; however, head‐to‐head evaluation of specific zwitterionic ligands for cell loading has not yet been explored. Magnetic particle imaging (MPI) detects superparamagnetic iron oxide nanoparticles (SPIONs) using time‐varying magnetic fields. Because MPI can produce high‐contrast, real‐time images with no tissue depth limitation, it is an ideal candidate for in vivo cell tracking. In this work, we have conjugated hard (permanently charged) and soft (pKa‐dependently charged) biomimetic zwitterionic ligands to SPIONs and characterized how these ligands changed SPION physicochemical properties. We have evaluated cellular uptake and subcellular localization between zwitterions, how the improvement in cell uptake generated stronger MPI signal for smaller numbers of cells, and how these cells can be tracked in an animal model with greater sensitivity for longer periods of time. Our best‐performing surface coating afforded high cell loading within 4 h, with full signal retention in vivo over 7 days.https://doi.org/10.1002/agt2.609cell trackingmagnetic nanoparticle imaging (MPI)nanoparticle functionalizationnanoparticle uptakesurface chemistryzwitterionic ligands |
| spellingShingle | Nicholas D. Calvert Luciana Yu Olivia C. Sehl Julia J. Gevaert Natasha N. Knier Angelie Rivera‐Rodriguez Clara S. Goulet Nitara Fernando Samantha Flood Carlos M. Rinaldi‐Ramos Paula J. Foster Adam J. Shuhendler The careful selection of zwitterionic nanoparticle coating results in rapid and efficient cell labeling for imaging‐based cell tracking Aggregate cell tracking magnetic nanoparticle imaging (MPI) nanoparticle functionalization nanoparticle uptake surface chemistry zwitterionic ligands |
| title | The careful selection of zwitterionic nanoparticle coating results in rapid and efficient cell labeling for imaging‐based cell tracking |
| title_full | The careful selection of zwitterionic nanoparticle coating results in rapid and efficient cell labeling for imaging‐based cell tracking |
| title_fullStr | The careful selection of zwitterionic nanoparticle coating results in rapid and efficient cell labeling for imaging‐based cell tracking |
| title_full_unstemmed | The careful selection of zwitterionic nanoparticle coating results in rapid and efficient cell labeling for imaging‐based cell tracking |
| title_short | The careful selection of zwitterionic nanoparticle coating results in rapid and efficient cell labeling for imaging‐based cell tracking |
| title_sort | careful selection of zwitterionic nanoparticle coating results in rapid and efficient cell labeling for imaging based cell tracking |
| topic | cell tracking magnetic nanoparticle imaging (MPI) nanoparticle functionalization nanoparticle uptake surface chemistry zwitterionic ligands |
| url | https://doi.org/10.1002/agt2.609 |
| work_keys_str_mv | AT nicholasdcalvert thecarefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT lucianayu thecarefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT oliviacsehl thecarefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT juliajgevaert thecarefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT natashanknier thecarefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT angelieriverarodriguez thecarefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT clarasgoulet thecarefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT nitarafernando thecarefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT samanthaflood thecarefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT carlosmrinaldiramos thecarefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT paulajfoster thecarefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT adamjshuhendler thecarefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT nicholasdcalvert carefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT lucianayu carefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT oliviacsehl carefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT juliajgevaert carefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT natashanknier carefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT angelieriverarodriguez carefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT clarasgoulet carefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT nitarafernando carefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT samanthaflood carefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT carlosmrinaldiramos carefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT paulajfoster carefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking AT adamjshuhendler carefulselectionofzwitterionicnanoparticlecoatingresultsinrapidandefficientcelllabelingforimagingbasedcelltracking |