The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation
Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for differentiation into diverse tissues. We report a straightforward and highly efficient method for the generation of iPSCs from PBMCs. By plating the cells serially to a newly coated plate by centrifugation, this prot...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/1329459 |
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| author | Youngkyun Kim Yeri Alice Rim Hyoju Yi Narae Park Sung-Hwan Park Ji Hyeon Ju |
| author_facet | Youngkyun Kim Yeri Alice Rim Hyoju Yi Narae Park Sung-Hwan Park Ji Hyeon Ju |
| author_sort | Youngkyun Kim |
| collection | DOAJ |
| description | Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for differentiation into diverse tissues. We report a straightforward and highly efficient method for the generation of iPSCs from PBMCs. By plating the cells serially to a newly coated plate by centrifugation, this protocol provides multiple healthy iPSC colonies even from a small number of PBMCs. The generated iPSCs expressed pluripotent markers and differentiated into all three germ layer lineages. The protocol can also be used with umbilical cord blood mononuclear cells (CBMCs). In this study, we present a simple and efficient protocol that improved the yield of iPSCs from floating cells such as PBMCs and CBMCs by serial plating and centrifugation. |
| format | Article |
| id | doaj-art-719eac5cc5e04becb2f2c3da558168e8 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-719eac5cc5e04becb2f2c3da558168e82025-08-20T02:06:32ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/13294591329459The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by CentrifugationYoungkyun Kim0Yeri Alice Rim1Hyoju Yi2Narae Park3Sung-Hwan Park4Ji Hyeon Ju5Division of Cardiology, Department of Medicine, Institute for Stem Cell Biology and Regenerative Medicine and Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USACiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary’s Hospital, College of Medicine, Catholic University of Korea, Seoul 137-701, Republic of KoreaDivision of Cardiology, Department of Medicine, Institute for Stem Cell Biology and Regenerative Medicine and Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USACiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary’s Hospital, College of Medicine, Catholic University of Korea, Seoul 137-701, Republic of KoreaDivision of Rheumatology, Department of Internal Medicine, Seoul St. Mary’s Hospital, Institute of Medical Science, College of Medicine, Catholic University of Korea, Seoul 137-701, Republic of KoreaCiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary’s Hospital, College of Medicine, Catholic University of Korea, Seoul 137-701, Republic of KoreaHuman induced pluripotent stem cells (hiPSCs) have demonstrated great potential for differentiation into diverse tissues. We report a straightforward and highly efficient method for the generation of iPSCs from PBMCs. By plating the cells serially to a newly coated plate by centrifugation, this protocol provides multiple healthy iPSC colonies even from a small number of PBMCs. The generated iPSCs expressed pluripotent markers and differentiated into all three germ layer lineages. The protocol can also be used with umbilical cord blood mononuclear cells (CBMCs). In this study, we present a simple and efficient protocol that improved the yield of iPSCs from floating cells such as PBMCs and CBMCs by serial plating and centrifugation.http://dx.doi.org/10.1155/2016/1329459 |
| spellingShingle | Youngkyun Kim Yeri Alice Rim Hyoju Yi Narae Park Sung-Hwan Park Ji Hyeon Ju The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation Stem Cells International |
| title | The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation |
| title_full | The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation |
| title_fullStr | The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation |
| title_full_unstemmed | The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation |
| title_short | The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation |
| title_sort | generation of human induced pluripotent stem cells from blood cells an efficient protocol using serial plating of reprogrammed cells by centrifugation |
| url | http://dx.doi.org/10.1155/2016/1329459 |
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