Renal protective effects of helix B surface polypeptide in rats with puromycin aminonucleoside nephropathy
Background Recent studies have reported that helix B surface polypeptide (HBSP), an erythropoietin derivative, exhibits strong tissue protective effects, independent of erythropoietic effects, in a renal ischemia-reperfusion (IR) injury model. Meanwhile, the transforming growth factor-β (TGF-β) supe...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Renal Failure |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2394637 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850109152863453184 |
|---|---|
| author | Ting Chen Xiao-ye Shen Hui-min Liang Hui Shi Li Yuan |
| author_facet | Ting Chen Xiao-ye Shen Hui-min Liang Hui Shi Li Yuan |
| author_sort | Ting Chen |
| collection | DOAJ |
| description | Background Recent studies have reported that helix B surface polypeptide (HBSP), an erythropoietin derivative, exhibits strong tissue protective effects, independent of erythropoietic effects, in a renal ischemia-reperfusion (IR) injury model. Meanwhile, the transforming growth factor-β (TGF-β) superfamily member glial cell line-derived neurotrophic factor (GDNF) demonstrated protective effect on podocytes in vitro. Using a rat puromycin aminonucleoside nephropathy (PAN) model, this study observed the renal protective effect of HBSP and investigated its renal protective effect on podocytes and mechanism related to GDNF.Methods Rats nephropathy model was induced by injection of 60 mg/kg of PAN via the tail vein. Rats in the PAN + HBSP group were injected intraperitoneally with HBSP (8 nmol/kg) 4 h before the model was induced, followed by intraperitoneal injections of HBSP once every 24 h for 7 consecutive days. The 24-hour urinary protein level was measured once every other day, and blood and renal tissue samples were collected on the 7th day for the examination of renal function, complete blood count, renal pathological changes and the expression levels of GDNF.Results Compared with the control group, the PAN nephropathy rat model showed a large amount of urinary protein. The pathological manifestations were mainly extensive fusion and disappearance of foot processes, along with vacuolar degeneration of podocytes and their separation from the glomerular basement membrane. GDNF expression was upregulated. Compared with the PAN + vehicle group, the PAN + HBSP group showed decreased urinary protein (p < 0.05). Pathological examination revealed ameliorated glomerular injury and vacuolar degeneration of podocytes. The expression of GDNF in the PAN nephropathy group was increased, when compared with the control group. The greatest expression of GDNF observed in the PAN + HBSP group (p < 0.05).Conclusions The expression of GDNF in the kidney of PAN rat model was increased. HBSP reduced urinary protein, ameliorated pathological changes in renal podocytes, increased the expression of GDNF in the PAN rat model. HBSP is likely to exert its protective effects on podocytes through upregulation of GDNF expression. |
| format | Article |
| id | doaj-art-718bdfec07d04de398360de0803ebf9a |
| institution | OA Journals |
| issn | 0886-022X 1525-6049 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj-art-718bdfec07d04de398360de0803ebf9a2025-08-20T02:38:10ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492024-12-0146210.1080/0886022X.2024.2394637Renal protective effects of helix B surface polypeptide in rats with puromycin aminonucleoside nephropathyTing Chen0Xiao-ye Shen1Hui-min Liang2Hui Shi3Li Yuan4Division of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, ChinaDivision of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, ChinaDivision of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, ChinaDivision of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, ChinaDivision of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, ChinaBackground Recent studies have reported that helix B surface polypeptide (HBSP), an erythropoietin derivative, exhibits strong tissue protective effects, independent of erythropoietic effects, in a renal ischemia-reperfusion (IR) injury model. Meanwhile, the transforming growth factor-β (TGF-β) superfamily member glial cell line-derived neurotrophic factor (GDNF) demonstrated protective effect on podocytes in vitro. Using a rat puromycin aminonucleoside nephropathy (PAN) model, this study observed the renal protective effect of HBSP and investigated its renal protective effect on podocytes and mechanism related to GDNF.Methods Rats nephropathy model was induced by injection of 60 mg/kg of PAN via the tail vein. Rats in the PAN + HBSP group were injected intraperitoneally with HBSP (8 nmol/kg) 4 h before the model was induced, followed by intraperitoneal injections of HBSP once every 24 h for 7 consecutive days. The 24-hour urinary protein level was measured once every other day, and blood and renal tissue samples were collected on the 7th day for the examination of renal function, complete blood count, renal pathological changes and the expression levels of GDNF.Results Compared with the control group, the PAN nephropathy rat model showed a large amount of urinary protein. The pathological manifestations were mainly extensive fusion and disappearance of foot processes, along with vacuolar degeneration of podocytes and their separation from the glomerular basement membrane. GDNF expression was upregulated. Compared with the PAN + vehicle group, the PAN + HBSP group showed decreased urinary protein (p < 0.05). Pathological examination revealed ameliorated glomerular injury and vacuolar degeneration of podocytes. The expression of GDNF in the PAN nephropathy group was increased, when compared with the control group. The greatest expression of GDNF observed in the PAN + HBSP group (p < 0.05).Conclusions The expression of GDNF in the kidney of PAN rat model was increased. HBSP reduced urinary protein, ameliorated pathological changes in renal podocytes, increased the expression of GDNF in the PAN rat model. HBSP is likely to exert its protective effects on podocytes through upregulation of GDNF expression.https://www.tandfonline.com/doi/10.1080/0886022X.2024.2394637Glial cell line-derived neurotrophic factorhelix B surface polypeptidepuromycin aminonucleoside nephropathypodocyte |
| spellingShingle | Ting Chen Xiao-ye Shen Hui-min Liang Hui Shi Li Yuan Renal protective effects of helix B surface polypeptide in rats with puromycin aminonucleoside nephropathy Renal Failure Glial cell line-derived neurotrophic factor helix B surface polypeptide puromycin aminonucleoside nephropathy podocyte |
| title | Renal protective effects of helix B surface polypeptide in rats with puromycin aminonucleoside nephropathy |
| title_full | Renal protective effects of helix B surface polypeptide in rats with puromycin aminonucleoside nephropathy |
| title_fullStr | Renal protective effects of helix B surface polypeptide in rats with puromycin aminonucleoside nephropathy |
| title_full_unstemmed | Renal protective effects of helix B surface polypeptide in rats with puromycin aminonucleoside nephropathy |
| title_short | Renal protective effects of helix B surface polypeptide in rats with puromycin aminonucleoside nephropathy |
| title_sort | renal protective effects of helix b surface polypeptide in rats with puromycin aminonucleoside nephropathy |
| topic | Glial cell line-derived neurotrophic factor helix B surface polypeptide puromycin aminonucleoside nephropathy podocyte |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2394637 |
| work_keys_str_mv | AT tingchen renalprotectiveeffectsofhelixbsurfacepolypeptideinratswithpuromycinaminonucleosidenephropathy AT xiaoyeshen renalprotectiveeffectsofhelixbsurfacepolypeptideinratswithpuromycinaminonucleosidenephropathy AT huiminliang renalprotectiveeffectsofhelixbsurfacepolypeptideinratswithpuromycinaminonucleosidenephropathy AT huishi renalprotectiveeffectsofhelixbsurfacepolypeptideinratswithpuromycinaminonucleosidenephropathy AT liyuan renalprotectiveeffectsofhelixbsurfacepolypeptideinratswithpuromycinaminonucleosidenephropathy |