Genetic relatedness of clinical and environmental Acinetobacter baumanii isolates from an intensive care unit outbreak
Introduction: Determination of microbial genetic relatedness is important for improving efficiency of infection control measures during hospital outbreaks. This study aimed to analyze the clonal relationships of clinical and environmental Acinetobacter baumannii strains isolated during an outbreak i...
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The Journal of Infection in Developing Countries
2015-07-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/6726 |
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| author | Abiola Senok Ghada Garaween Adeola Raji Harish Khubnani Garwin Kim Sing Atef Shibl |
| author_facet | Abiola Senok Ghada Garaween Adeola Raji Harish Khubnani Garwin Kim Sing Atef Shibl |
| author_sort | Abiola Senok |
| collection | DOAJ |
| description | Introduction: Determination of microbial genetic relatedness is important for improving efficiency of infection control measures during hospital outbreaks. This study aimed to analyze the clonal relationships of clinical and environmental Acinetobacter baumannii strains isolated during an outbreak in the intensive care unit (ICU) of a secondary care hospital in Saudi Arabia.
Methodology: Twelve clinical and fourteen environmental A. baumannii isolates identified during an outbreak in February 2013 in the 14-bed adult intensive care unit of a tertiary care hospital in Riyadh, Saudi Arabia, were studied. Bacterial identification and antimicrobial susceptibility testing were carried out using Microscan Walkaway 96 automated system. Determination of clonal diversity was investigated by repetitive-sequence-based polymerase chain reaction (rep-PCR) with the semi-automated DiversiLab system.
Results: The majority of the clinical isolates were from endotracheal tube aspirates (n = 9), one from a wound swab and two were from urine and sputum, respectively. Environmental isolates were from bed railings (n = 10) and with one each from curtain, stethoscope, computer mouse and telephone. Isolates were categorized into 6 clusters (Groups 1-6). Most of the isolates were associated with two clusters namely Groups 2 (n = 11) and 3 (n = 9). All isolates were multidrug resistant showing resistance to three or more classes of antibiotics. One clinical strain from Cluster 3 was resistant to colistin (MIC > 4ug/ml).
Conclusion: This outbreak was caused by two clonal groups of multidrug resistant A. baumannii. The presence of multiple environmental clones was suggestive of environmental source dissemination via healthcare workers within the ICU.
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| format | Article |
| id | doaj-art-7189e94cdd9443cf85274e7f4fdb546d |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-7189e94cdd9443cf85274e7f4fdb546d2025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-07-0190610.3855/jidc.6726Genetic relatedness of clinical and environmental Acinetobacter baumanii isolates from an intensive care unit outbreakAbiola Senok0Ghada Garaween1Adeola Raji2Harish Khubnani3Garwin Kim Sing4Atef Shibl5College of Medicine, Alfaisal University, Riyadh, Saudi ArabiaCollege of Medicine, Alfaisal University, Riyadh, Saudi ArabiaCollege of Medicine, Alfaisal University, Riyadh, Saudi ArabiaKing Faisal Specialist Hospital and Research Center, Riyadh, Saudi ArabiaCollege of Medicine, Alfaisal University, Riyadh, Saudi ArabiaCollege of Medicine, Alfaisal University, Riyadh, Saudi ArabiaIntroduction: Determination of microbial genetic relatedness is important for improving efficiency of infection control measures during hospital outbreaks. This study aimed to analyze the clonal relationships of clinical and environmental Acinetobacter baumannii strains isolated during an outbreak in the intensive care unit (ICU) of a secondary care hospital in Saudi Arabia. Methodology: Twelve clinical and fourteen environmental A. baumannii isolates identified during an outbreak in February 2013 in the 14-bed adult intensive care unit of a tertiary care hospital in Riyadh, Saudi Arabia, were studied. Bacterial identification and antimicrobial susceptibility testing were carried out using Microscan Walkaway 96 automated system. Determination of clonal diversity was investigated by repetitive-sequence-based polymerase chain reaction (rep-PCR) with the semi-automated DiversiLab system. Results: The majority of the clinical isolates were from endotracheal tube aspirates (n = 9), one from a wound swab and two were from urine and sputum, respectively. Environmental isolates were from bed railings (n = 10) and with one each from curtain, stethoscope, computer mouse and telephone. Isolates were categorized into 6 clusters (Groups 1-6). Most of the isolates were associated with two clusters namely Groups 2 (n = 11) and 3 (n = 9). All isolates were multidrug resistant showing resistance to three or more classes of antibiotics. One clinical strain from Cluster 3 was resistant to colistin (MIC > 4ug/ml). Conclusion: This outbreak was caused by two clonal groups of multidrug resistant A. baumannii. The presence of multiple environmental clones was suggestive of environmental source dissemination via healthcare workers within the ICU. https://jidc.org/index.php/journal/article/view/6726Acinetobacter baumanniirep-PCRDiversiLabinfection control |
| spellingShingle | Abiola Senok Ghada Garaween Adeola Raji Harish Khubnani Garwin Kim Sing Atef Shibl Genetic relatedness of clinical and environmental Acinetobacter baumanii isolates from an intensive care unit outbreak Journal of Infection in Developing Countries Acinetobacter baumannii rep-PCR DiversiLab infection control |
| title | Genetic relatedness of clinical and environmental Acinetobacter baumanii isolates from an intensive care unit outbreak |
| title_full | Genetic relatedness of clinical and environmental Acinetobacter baumanii isolates from an intensive care unit outbreak |
| title_fullStr | Genetic relatedness of clinical and environmental Acinetobacter baumanii isolates from an intensive care unit outbreak |
| title_full_unstemmed | Genetic relatedness of clinical and environmental Acinetobacter baumanii isolates from an intensive care unit outbreak |
| title_short | Genetic relatedness of clinical and environmental Acinetobacter baumanii isolates from an intensive care unit outbreak |
| title_sort | genetic relatedness of clinical and environmental acinetobacter baumanii isolates from an intensive care unit outbreak |
| topic | Acinetobacter baumannii rep-PCR DiversiLab infection control |
| url | https://jidc.org/index.php/journal/article/view/6726 |
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