Development and characterization of a pseudovirus system for human Aichi virus: in vitro and in vivo analysis
Abstract Human Aichi virus (AiV) (genus Kobuvirus, family Picornaviridae) has been described as a causative agent of human gastroenteritis since 1989. However, research on AiV at cellular and animal levels is limited. Utilizing a double reporter gene system, we constructed an AiV capsid protein plas...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
|
| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-025-02839-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849388356572545024 |
|---|---|
| author | Ruojun Wu Jingli Tian Shunchang Fan Minyi Liang Yucheng Li Ying Deng Binbin Tang Minyi Zhang Qing Chen |
| author_facet | Ruojun Wu Jingli Tian Shunchang Fan Minyi Liang Yucheng Li Ying Deng Binbin Tang Minyi Zhang Qing Chen |
| author_sort | Ruojun Wu |
| collection | DOAJ |
| description | Abstract Human Aichi virus (AiV) (genus Kobuvirus, family Picornaviridae) has been described as a causative agent of human gastroenteritis since 1989. However, research on AiV at cellular and animal levels is limited. Utilizing a double reporter gene system, we constructed an AiV capsid protein plasmid and genomic backbone Replicon. Subsequently, AiV pseudovirus (AiV PsV) particles were packaged using a three-plasmid co-transfection system. Eleven cell types were screened to identify those susceptible to AiV PsV. Mouse models were established with AiV PsV to determine the optimal mouse species, mode of infection, and detection time, and investigate distribution characteristics of AiVs in vivo. HeLa cells exhibited the highest sensitivity to AiV PsV. A BALB/c mouse model established with bioluminescence imaging performed 24 h after infection via intraperitoneal injection demonstrated that the bioluminescent signal was concentrated in the murine abdominal cavity. The AiV PsV system should advance understanding of the infectious features of AiVs. |
| format | Article |
| id | doaj-art-7181caf05d6440f79e9cd16ed146c6e8 |
| institution | Kabale University |
| issn | 1743-422X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj-art-7181caf05d6440f79e9cd16ed146c6e82025-08-20T03:42:19ZengBMCVirology Journal1743-422X2025-07-0122111010.1186/s12985-025-02839-yDevelopment and characterization of a pseudovirus system for human Aichi virus: in vitro and in vivo analysisRuojun Wu0Jingli Tian1Shunchang Fan2Minyi Liang3Yucheng Li4Ying Deng5Binbin Tang6Minyi Zhang7Qing Chen8Department of Epidemiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical UniversityDepartment of Epidemiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical UniversityDepartment of Epidemiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical UniversityDepartment of Epidemiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical UniversityDepartment of Epidemiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical UniversityDepartment of Epidemiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical UniversityDepartment of Epidemiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical UniversityDepartment of Epidemiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical UniversityDepartment of Epidemiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical UniversityAbstract Human Aichi virus (AiV) (genus Kobuvirus, family Picornaviridae) has been described as a causative agent of human gastroenteritis since 1989. However, research on AiV at cellular and animal levels is limited. Utilizing a double reporter gene system, we constructed an AiV capsid protein plasmid and genomic backbone Replicon. Subsequently, AiV pseudovirus (AiV PsV) particles were packaged using a three-plasmid co-transfection system. Eleven cell types were screened to identify those susceptible to AiV PsV. Mouse models were established with AiV PsV to determine the optimal mouse species, mode of infection, and detection time, and investigate distribution characteristics of AiVs in vivo. HeLa cells exhibited the highest sensitivity to AiV PsV. A BALB/c mouse model established with bioluminescence imaging performed 24 h after infection via intraperitoneal injection demonstrated that the bioluminescent signal was concentrated in the murine abdominal cavity. The AiV PsV system should advance understanding of the infectious features of AiVs.https://doi.org/10.1186/s12985-025-02839-yHuman Aichi virusPseudovirusesCellsMouse modelBioluminescent imaging |
| spellingShingle | Ruojun Wu Jingli Tian Shunchang Fan Minyi Liang Yucheng Li Ying Deng Binbin Tang Minyi Zhang Qing Chen Development and characterization of a pseudovirus system for human Aichi virus: in vitro and in vivo analysis Virology Journal Human Aichi virus Pseudoviruses Cells Mouse model Bioluminescent imaging |
| title | Development and characterization of a pseudovirus system for human Aichi virus: in vitro and in vivo analysis |
| title_full | Development and characterization of a pseudovirus system for human Aichi virus: in vitro and in vivo analysis |
| title_fullStr | Development and characterization of a pseudovirus system for human Aichi virus: in vitro and in vivo analysis |
| title_full_unstemmed | Development and characterization of a pseudovirus system for human Aichi virus: in vitro and in vivo analysis |
| title_short | Development and characterization of a pseudovirus system for human Aichi virus: in vitro and in vivo analysis |
| title_sort | development and characterization of a pseudovirus system for human aichi virus in vitro and in vivo analysis |
| topic | Human Aichi virus Pseudoviruses Cells Mouse model Bioluminescent imaging |
| url | https://doi.org/10.1186/s12985-025-02839-y |
| work_keys_str_mv | AT ruojunwu developmentandcharacterizationofapseudovirussystemforhumanaichivirusinvitroandinvivoanalysis AT jinglitian developmentandcharacterizationofapseudovirussystemforhumanaichivirusinvitroandinvivoanalysis AT shunchangfan developmentandcharacterizationofapseudovirussystemforhumanaichivirusinvitroandinvivoanalysis AT minyiliang developmentandcharacterizationofapseudovirussystemforhumanaichivirusinvitroandinvivoanalysis AT yuchengli developmentandcharacterizationofapseudovirussystemforhumanaichivirusinvitroandinvivoanalysis AT yingdeng developmentandcharacterizationofapseudovirussystemforhumanaichivirusinvitroandinvivoanalysis AT binbintang developmentandcharacterizationofapseudovirussystemforhumanaichivirusinvitroandinvivoanalysis AT minyizhang developmentandcharacterizationofapseudovirussystemforhumanaichivirusinvitroandinvivoanalysis AT qingchen developmentandcharacterizationofapseudovirussystemforhumanaichivirusinvitroandinvivoanalysis |