Differences in IgG afucosylation between groups with and without carotid atherosclerosis

Abstract Background A previous study demonstrated that N-glycosylation profiles of IgG are associated with subclinical atherosclerosis in a British population. However, the generalisability of this finding to other ethnic groups remains to be investigated, and it has yet to account for additional tr...

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Main Authors: Cuihong Tian, Xingang Li, Hongxia Zhang, Jieyi He, Yan Zhou, Manshu Song, Peixuan Yang, Xuerui Tan
Format: Article
Language:English
Published: BMC 2024-11-01
Series:BMC Cardiovascular Disorders
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Online Access:https://doi.org/10.1186/s12872-024-04296-x
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author Cuihong Tian
Xingang Li
Hongxia Zhang
Jieyi He
Yan Zhou
Manshu Song
Peixuan Yang
Xuerui Tan
author_facet Cuihong Tian
Xingang Li
Hongxia Zhang
Jieyi He
Yan Zhou
Manshu Song
Peixuan Yang
Xuerui Tan
author_sort Cuihong Tian
collection DOAJ
description Abstract Background A previous study demonstrated that N-glycosylation profiles of IgG are associated with subclinical atherosclerosis in a British population. However, the generalisability of this finding to other ethnic groups remains to be investigated, and it has yet to account for additional traditional atherosclerotic risk factors. The present study, thus, aims to explore IgG N-glycosylation profiles in Han Chinese with atherosclerosis, and their potential role in atherosclerosis, while controlling for traditional atherosclerotic risk factors. Methods Data of this case-control study were obtained from an established umbrella Health Examination Cohort Study (registration number: ChiCTR2100048740). The investigation was conducted at the Health Care Centre of the First Affiliated Hospital of Shantou University Medical College in China, from August 1, 2021, to July 31, 2022. A sample of 69 carotid atherosclerosis (CAS) cases was recruited from the umbrella cohort, along with 69 controls without carotid atherosclerosis, matched by traditional atherosclerosis-related risk factors, including gender, age, smoking, alcohol consumption, hypertension, diabetes, dyslipidemia and obesity. Subsequently, serum IgG N-glycosylation was profiled using Ultra-Performance Liquid Chromatography. Results After propensity score matching, the relative abundance of IgG fucosylation in CAS cases was significantly lower than that in controls [95.32 (92.96, 95.99) vs. 95.96 (94.70, 96.58), P = 0.022]. The traditional atherosclerosis-related risk factors showed no statistically significant difference between CAS cases and controls (P > 0.05). Conclusions The reduced fucosylation of IgG in CAS cases underscores the pivotal role of afucosylation in CAS. Enhancing the inflammatory capability of IgG via initiating antibody-dependent cell-mediated cytotoxicity could be the potential mechanism behind this, which should be further verified by functional studies.
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spelling doaj-art-717676ca6308410fa82bed2c890f44fe2025-08-20T02:18:20ZengBMCBMC Cardiovascular Disorders1471-22612024-11-012411810.1186/s12872-024-04296-xDifferences in IgG afucosylation between groups with and without carotid atherosclerosisCuihong Tian0Xingang Li1Hongxia Zhang2Jieyi He3Yan Zhou4Manshu Song5Peixuan Yang6Xuerui Tan7Department of Cardiology, First Affiliated Hospital of Shantou University Medical CollegeCentre for Precision Health, Edith Cowan UniversityHealth Care Centre, First Affiliated Hospital of Shantou University Medical CollegeHealth Care Centre, First Affiliated Hospital of Shantou University Medical CollegeDepartment of Critical Care Medicine, Shenzhen Second People’s Hospital, First Affiliated Hospital of Shenzhen UniversityCentre for Precision Health, Edith Cowan UniversityHealth Care Centre, First Affiliated Hospital of Shantou University Medical CollegeDepartment of Cardiology, First Affiliated Hospital of Shantou University Medical CollegeAbstract Background A previous study demonstrated that N-glycosylation profiles of IgG are associated with subclinical atherosclerosis in a British population. However, the generalisability of this finding to other ethnic groups remains to be investigated, and it has yet to account for additional traditional atherosclerotic risk factors. The present study, thus, aims to explore IgG N-glycosylation profiles in Han Chinese with atherosclerosis, and their potential role in atherosclerosis, while controlling for traditional atherosclerotic risk factors. Methods Data of this case-control study were obtained from an established umbrella Health Examination Cohort Study (registration number: ChiCTR2100048740). The investigation was conducted at the Health Care Centre of the First Affiliated Hospital of Shantou University Medical College in China, from August 1, 2021, to July 31, 2022. A sample of 69 carotid atherosclerosis (CAS) cases was recruited from the umbrella cohort, along with 69 controls without carotid atherosclerosis, matched by traditional atherosclerosis-related risk factors, including gender, age, smoking, alcohol consumption, hypertension, diabetes, dyslipidemia and obesity. Subsequently, serum IgG N-glycosylation was profiled using Ultra-Performance Liquid Chromatography. Results After propensity score matching, the relative abundance of IgG fucosylation in CAS cases was significantly lower than that in controls [95.32 (92.96, 95.99) vs. 95.96 (94.70, 96.58), P = 0.022]. The traditional atherosclerosis-related risk factors showed no statistically significant difference between CAS cases and controls (P > 0.05). Conclusions The reduced fucosylation of IgG in CAS cases underscores the pivotal role of afucosylation in CAS. Enhancing the inflammatory capability of IgG via initiating antibody-dependent cell-mediated cytotoxicity could be the potential mechanism behind this, which should be further verified by functional studies.https://doi.org/10.1186/s12872-024-04296-xAtherosclerosisCarotid atherosclerosisIgG N-glycosylation profilesHan Chinese populationPropensity score matchingCase-control study
spellingShingle Cuihong Tian
Xingang Li
Hongxia Zhang
Jieyi He
Yan Zhou
Manshu Song
Peixuan Yang
Xuerui Tan
Differences in IgG afucosylation between groups with and without carotid atherosclerosis
BMC Cardiovascular Disorders
Atherosclerosis
Carotid atherosclerosis
IgG N-glycosylation profiles
Han Chinese population
Propensity score matching
Case-control study
title Differences in IgG afucosylation between groups with and without carotid atherosclerosis
title_full Differences in IgG afucosylation between groups with and without carotid atherosclerosis
title_fullStr Differences in IgG afucosylation between groups with and without carotid atherosclerosis
title_full_unstemmed Differences in IgG afucosylation between groups with and without carotid atherosclerosis
title_short Differences in IgG afucosylation between groups with and without carotid atherosclerosis
title_sort differences in igg afucosylation between groups with and without carotid atherosclerosis
topic Atherosclerosis
Carotid atherosclerosis
IgG N-glycosylation profiles
Han Chinese population
Propensity score matching
Case-control study
url https://doi.org/10.1186/s12872-024-04296-x
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