Quercetin Alleviates Hyperglycemic-Generated Endoplasmic Reticulum Stress-Contacted Apoptosis of Rat Nucleus Pulposus Cells
Background: Previous research has shown that apoptosis of nucleus pulposus (NP) cells contributes to intervertebral disc degeneration (IDD) progression. Endoplasmic reticulum (ER) stress is a reaction to diverse stimuli in eukaryotes and is tightly contacted with apoptosis. Quercetin, a naturally oc...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2025-01-01
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| Series: | Balkan Medical Journal |
| Online Access: | https://balkanmedicaljournal.org/abstract.php?id=2668 |
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| author | Zhen Yu Xianfeng Wang Yusen Hu Xinfa Wang Zhenghuan Zhu Xu Xu Yiming Wang Ailiang Zhang |
| author_facet | Zhen Yu Xianfeng Wang Yusen Hu Xinfa Wang Zhenghuan Zhu Xu Xu Yiming Wang Ailiang Zhang |
| author_sort | Zhen Yu |
| collection | DOAJ |
| description | Background: Previous research has shown that apoptosis of nucleus pulposus (NP) cells contributes to intervertebral disc degeneration (IDD) progression. Endoplasmic reticulum (ER) stress is a reaction to diverse stimuli in eukaryotes and is tightly contacted with apoptosis. Quercetin, a naturally occurring flavonoid, exerts protective effects against degenerative diseases via ER stress. However, the effect of quercetin on NP cell apoptosis remains unclear.
Aims: To investigate the influences of quercetin on apoptosis and ER stress in a high-glucose-generated primary NP cell model.
Study Design: In vivo animal experimental study.
Methods: To investigate the influence of quercetin in a high-glucose-generated NP cell apoptosis model, control, glucose, and glucose + quercetin groups adopted with Sprague-Dawley rats primary NP cells. In the glucose group, cell apoptosis was generated by 200 mm high glucose. In the glucose + quercetin group, 60 µm quercetin was pretreated with NP cells for 2 h before glucose administration. In this research, we examined the change effect of quercetin on NP cell apoptosis, ER stress, and the protein kinase R-like ER kinase (PERK)-eukaryotic translation initiation element 2α (eIF2α)-activating transcription element 4 (ATF4).
Results: High glucose decreased the viability and induced ER stress-related apoptosis in NP cells. Quercetin modulated ER stress through the PERK-eIF2α-ATF4 pathway, thereby alleviating the apoptosis rank in NP cells.
Conclusion: Quercetin exerts antiapoptotic effects on NP cells, probably through ER stress, thereby showcasing potential as a therapeutic method for treating IDD. |
| format | Article |
| id | doaj-art-716bb270eaf747fb9fb71f0aff98c229 |
| institution | Kabale University |
| issn | 2146-3123 2146-3131 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Balkan Medical Journal |
| spelling | doaj-art-716bb270eaf747fb9fb71f0aff98c2292025-01-07T12:19:50ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312025-01-01421273610.4274/balkanmedj.galenos.2024.2024-7-92Quercetin Alleviates Hyperglycemic-Generated Endoplasmic Reticulum Stress-Contacted Apoptosis of Rat Nucleus Pulposus CellsZhen Yu0https://orcid.org/0000-0001-6614-9834Xianfeng Wang1https://orcid.org/0000-0002-1837-0670Yusen Hu2https://orcid.org/0009-0004-1090-8079Xinfa Wang3https://orcid.org/0009-0001-0149-5186Zhenghuan Zhu4https://orcid.org/0000-0002-1616-7580Xu Xu5https://orcid.org/0009-0005-3579-9099Yiming Wang6https://orcid.org/0000-0002-6215-8028Ailiang Zhang7https://orcid.org/0009-0003-6575-9579Department of Articular Orthopaedics, The Third Affiliated Hospital of Soochow University, Changzhou, ChinaDepartment of Orthopedic Surgery, Beijing Jishuitan Hospital Guizhou Hospital, Guiyang, ChinaDepartment of Spine Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, ChinaDepartment of Spine Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, ChinaDepartment of Spine Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, ChinaDepartment of Spine Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, ChinaDepartment of Articular Orthopaedics, The Third Affiliated Hospital of Soochow University, Changzhou, ChinaDepartment of Spine Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, ChinaBackground: Previous research has shown that apoptosis of nucleus pulposus (NP) cells contributes to intervertebral disc degeneration (IDD) progression. Endoplasmic reticulum (ER) stress is a reaction to diverse stimuli in eukaryotes and is tightly contacted with apoptosis. Quercetin, a naturally occurring flavonoid, exerts protective effects against degenerative diseases via ER stress. However, the effect of quercetin on NP cell apoptosis remains unclear. Aims: To investigate the influences of quercetin on apoptosis and ER stress in a high-glucose-generated primary NP cell model. Study Design: In vivo animal experimental study. Methods: To investigate the influence of quercetin in a high-glucose-generated NP cell apoptosis model, control, glucose, and glucose + quercetin groups adopted with Sprague-Dawley rats primary NP cells. In the glucose group, cell apoptosis was generated by 200 mm high glucose. In the glucose + quercetin group, 60 µm quercetin was pretreated with NP cells for 2 h before glucose administration. In this research, we examined the change effect of quercetin on NP cell apoptosis, ER stress, and the protein kinase R-like ER kinase (PERK)-eukaryotic translation initiation element 2α (eIF2α)-activating transcription element 4 (ATF4). Results: High glucose decreased the viability and induced ER stress-related apoptosis in NP cells. Quercetin modulated ER stress through the PERK-eIF2α-ATF4 pathway, thereby alleviating the apoptosis rank in NP cells. Conclusion: Quercetin exerts antiapoptotic effects on NP cells, probably through ER stress, thereby showcasing potential as a therapeutic method for treating IDD.https://balkanmedicaljournal.org/abstract.php?id=2668 |
| spellingShingle | Zhen Yu Xianfeng Wang Yusen Hu Xinfa Wang Zhenghuan Zhu Xu Xu Yiming Wang Ailiang Zhang Quercetin Alleviates Hyperglycemic-Generated Endoplasmic Reticulum Stress-Contacted Apoptosis of Rat Nucleus Pulposus Cells Balkan Medical Journal |
| title | Quercetin Alleviates Hyperglycemic-Generated Endoplasmic Reticulum Stress-Contacted Apoptosis of Rat Nucleus Pulposus Cells |
| title_full | Quercetin Alleviates Hyperglycemic-Generated Endoplasmic Reticulum Stress-Contacted Apoptosis of Rat Nucleus Pulposus Cells |
| title_fullStr | Quercetin Alleviates Hyperglycemic-Generated Endoplasmic Reticulum Stress-Contacted Apoptosis of Rat Nucleus Pulposus Cells |
| title_full_unstemmed | Quercetin Alleviates Hyperglycemic-Generated Endoplasmic Reticulum Stress-Contacted Apoptosis of Rat Nucleus Pulposus Cells |
| title_short | Quercetin Alleviates Hyperglycemic-Generated Endoplasmic Reticulum Stress-Contacted Apoptosis of Rat Nucleus Pulposus Cells |
| title_sort | quercetin alleviates hyperglycemic generated endoplasmic reticulum stress contacted apoptosis of rat nucleus pulposus cells |
| url | https://balkanmedicaljournal.org/abstract.php?id=2668 |
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