Structure and immunomodulatory activity of Lentinus edodes polysaccharides modified by probiotic fermentation
Plant-based fermentations provide an untapped source for novel biotechnological applications. In this study, a probiotic named Lactobacillus fermentum 21828 was introduced to ferment Lentinus edodes. Polysaccharides were extracted from fermented and non-fermented L. edodes and purified via DEAE-52 a...
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Tsinghua University Press
2024-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023001283 |
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| author | Jingjing Liang Meina Zhang Xiaohan Li Yuan Yue Xiaowei Wang Mengzhen Han Tianli Yue Zhouli Wang Zhenpeng Gao |
| author_facet | Jingjing Liang Meina Zhang Xiaohan Li Yuan Yue Xiaowei Wang Mengzhen Han Tianli Yue Zhouli Wang Zhenpeng Gao |
| author_sort | Jingjing Liang |
| collection | DOAJ |
| description | Plant-based fermentations provide an untapped source for novel biotechnological applications. In this study, a probiotic named Lactobacillus fermentum 21828 was introduced to ferment Lentinus edodes. Polysaccharides were extracted from fermented and non-fermented L. edodes and purified via DEAE-52 and Sephadex G-100. The components designated F-LEP-2a and NF-LEP-2a were analyzed by FT-IR, HPGPC, HPAEC, SEM, GC-MS and NMR. The results revealed that probiotic fermentation increased the molecular weight from 1.16 × 104 Da to 1.87 × 104 Da and altered the proportions of glucose, galactose and mannose, in which glucose increased from 45.94 % to 48.16 %. Methylation analysis and NMR spectra indicated that F-LEP-2a and NF-LEP-2a had similar linkage patterns. Furthermore, their immunomodulatory activities were evaluated with immunosuppressive mice. NF-LEP and F-LEP improved immune organ indices, immunoglobulin (IgG and IgM) and cytokines concentrations; restored the antioxidation capacity of liver; and maintained the balance of gut microbiota. F-LEP displayed better moderating effects on the spleen index, immunoglobulin, cytokines and the diversity of gut microbiota than NF-LEP (200, 400 mg/kg). Our study provides an efficient and environment-friendly way for the structural modification of polysaccharides, which helps to enhance their biological activity and promote their wide application in food, medicine and other fields. |
| format | Article |
| id | doaj-art-7159db5b04ca4a188e5e96cd79ac6852 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-7159db5b04ca4a188e5e96cd79ac68522025-02-03T05:54:06ZengTsinghua University PressFood Science and Human Wellness2213-45302024-01-01131421433Structure and immunomodulatory activity of Lentinus edodes polysaccharides modified by probiotic fermentationJingjing Liang0Meina Zhang1Xiaohan Li2Yuan Yue3Xiaowei Wang4Mengzhen Han5Tianli Yue6Zhouli Wang7Zhenpeng Gao8College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, 712100, ChinaXi'an GaoXin No.1 High School, Xi'an 710065, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China; Corresponding author at: College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China.Plant-based fermentations provide an untapped source for novel biotechnological applications. In this study, a probiotic named Lactobacillus fermentum 21828 was introduced to ferment Lentinus edodes. Polysaccharides were extracted from fermented and non-fermented L. edodes and purified via DEAE-52 and Sephadex G-100. The components designated F-LEP-2a and NF-LEP-2a were analyzed by FT-IR, HPGPC, HPAEC, SEM, GC-MS and NMR. The results revealed that probiotic fermentation increased the molecular weight from 1.16 × 104 Da to 1.87 × 104 Da and altered the proportions of glucose, galactose and mannose, in which glucose increased from 45.94 % to 48.16 %. Methylation analysis and NMR spectra indicated that F-LEP-2a and NF-LEP-2a had similar linkage patterns. Furthermore, their immunomodulatory activities were evaluated with immunosuppressive mice. NF-LEP and F-LEP improved immune organ indices, immunoglobulin (IgG and IgM) and cytokines concentrations; restored the antioxidation capacity of liver; and maintained the balance of gut microbiota. F-LEP displayed better moderating effects on the spleen index, immunoglobulin, cytokines and the diversity of gut microbiota than NF-LEP (200, 400 mg/kg). Our study provides an efficient and environment-friendly way for the structural modification of polysaccharides, which helps to enhance their biological activity and promote their wide application in food, medicine and other fields.http://www.sciencedirect.com/science/article/pii/S2213453023001283Lentinus edodes polysaccharideLactobacillus fermentum fermentationStructural analysisImmunoregulatory activityNuclear magnetic resonance |
| spellingShingle | Jingjing Liang Meina Zhang Xiaohan Li Yuan Yue Xiaowei Wang Mengzhen Han Tianli Yue Zhouli Wang Zhenpeng Gao Structure and immunomodulatory activity of Lentinus edodes polysaccharides modified by probiotic fermentation Food Science and Human Wellness Lentinus edodes polysaccharide Lactobacillus fermentum fermentation Structural analysis Immunoregulatory activity Nuclear magnetic resonance |
| title | Structure and immunomodulatory activity of Lentinus edodes polysaccharides modified by probiotic fermentation |
| title_full | Structure and immunomodulatory activity of Lentinus edodes polysaccharides modified by probiotic fermentation |
| title_fullStr | Structure and immunomodulatory activity of Lentinus edodes polysaccharides modified by probiotic fermentation |
| title_full_unstemmed | Structure and immunomodulatory activity of Lentinus edodes polysaccharides modified by probiotic fermentation |
| title_short | Structure and immunomodulatory activity of Lentinus edodes polysaccharides modified by probiotic fermentation |
| title_sort | structure and immunomodulatory activity of lentinus edodes polysaccharides modified by probiotic fermentation |
| topic | Lentinus edodes polysaccharide Lactobacillus fermentum fermentation Structural analysis Immunoregulatory activity Nuclear magnetic resonance |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023001283 |
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