Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations
ABSTRACT For about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development. Its appeal lies in its simple handling and highly versatile application for a wide range of cell types—from bacteria to...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-01-01
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| Series: | Engineering in Life Sciences |
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| Online Access: | https://doi.org/10.1002/elsc.70001 |
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| author | Gesa Brauneck Dominik Engel Luca Antonia Grebe Maximilian Hoffmann Philipp Georg Lichtenberg Anne Neuß Marcel Mann Jorgen Barsett Magnus |
| author_facet | Gesa Brauneck Dominik Engel Luca Antonia Grebe Maximilian Hoffmann Philipp Georg Lichtenberg Anne Neuß Marcel Mann Jorgen Barsett Magnus |
| author_sort | Gesa Brauneck |
| collection | DOAJ |
| description | ABSTRACT For about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development. Its appeal lies in its simple handling and highly versatile application for a wide range of cell types—from bacteria to mammalian cells. In recent decades, extensive research has been conducted on the shake flask, to not perform processes blindly but to gain a deeper understanding of the various process parameters, phenomena, and their impact on the process. Although the characterization of the shake flask is now well‐established in literature, many publications show that this knowledge is often inadequately applied. Therefore, this review provides an overview of the current state of knowledge on various topics related to the shake flask. We first present the key process parameters and their influence on different physical phenomena, such as power input, the largely unknown in‐phase/out‐of‐phase phenomenon, as well as temperature and mass transfer. Then, the most common online monitoring systems that have been established for shake flasks are discussed. Finally, various pitfalls that often arise from inadequate knowledge of handling shake flask cultivations are discussed and guidance on how to avoid them is provided. |
| format | Article |
| id | doaj-art-7158190c2d844a449445474b91b1d4ec |
| institution | Kabale University |
| issn | 1618-0240 1618-2863 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj-art-7158190c2d844a449445474b91b1d4ec2025-01-30T06:40:30ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632025-01-01251n/an/a10.1002/elsc.70001Pitfalls in Early Bioprocess Development Using Shake Flask CultivationsGesa Brauneck0Dominik Engel1Luca Antonia Grebe2Maximilian Hoffmann3Philipp Georg Lichtenberg4Anne Neuß5Marcel Mann6Jorgen Barsett Magnus7AVT – Biochemical Engineering RWTH Aachen University Aachen GermanyAVT – Biochemical Engineering RWTH Aachen University Aachen GermanyAVT – Biochemical Engineering RWTH Aachen University Aachen GermanyAVT – Biochemical Engineering RWTH Aachen University Aachen GermanyAVT – Biochemical Engineering RWTH Aachen University Aachen GermanyAVT – Biochemical Engineering RWTH Aachen University Aachen GermanyAVT – Biochemical Engineering RWTH Aachen University Aachen GermanyAVT – Biochemical Engineering RWTH Aachen University Aachen GermanyABSTRACT For about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development. Its appeal lies in its simple handling and highly versatile application for a wide range of cell types—from bacteria to mammalian cells. In recent decades, extensive research has been conducted on the shake flask, to not perform processes blindly but to gain a deeper understanding of the various process parameters, phenomena, and their impact on the process. Although the characterization of the shake flask is now well‐established in literature, many publications show that this knowledge is often inadequately applied. Therefore, this review provides an overview of the current state of knowledge on various topics related to the shake flask. We first present the key process parameters and their influence on different physical phenomena, such as power input, the largely unknown in‐phase/out‐of‐phase phenomenon, as well as temperature and mass transfer. Then, the most common online monitoring systems that have been established for shake flasks are discussed. Finally, various pitfalls that often arise from inadequate knowledge of handling shake flask cultivations are discussed and guidance on how to avoid them is provided.https://doi.org/10.1002/elsc.70001biochemical engineeringbioprocess developmentcultivation systemonline monitoringshake flask |
| spellingShingle | Gesa Brauneck Dominik Engel Luca Antonia Grebe Maximilian Hoffmann Philipp Georg Lichtenberg Anne Neuß Marcel Mann Jorgen Barsett Magnus Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations Engineering in Life Sciences biochemical engineering bioprocess development cultivation system online monitoring shake flask |
| title | Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations |
| title_full | Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations |
| title_fullStr | Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations |
| title_full_unstemmed | Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations |
| title_short | Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations |
| title_sort | pitfalls in early bioprocess development using shake flask cultivations |
| topic | biochemical engineering bioprocess development cultivation system online monitoring shake flask |
| url | https://doi.org/10.1002/elsc.70001 |
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