In Ovo Feeding of Arginine, Leucine, and Methionine in Broiler Breeders’ Eggs During Summer: Effects on Hatchability and Chick Oxidation, Inflammation, and Apoptosis
Summer high temperatures (27–38 °C, more than 7 days) readily induce heat stress in late-laying broiler breeders, which impedes offspring growth and development. This study aimed to provide a scientific basis for improving the offspring development of late-laying broiler breeders during summer. Six...
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MDPI AG
2025-06-01
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| Series: | Animals |
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| author | Huan Ge Zhenwu Huang Jinghai Feng Minhong Zhang |
| author_facet | Huan Ge Zhenwu Huang Jinghai Feng Minhong Zhang |
| author_sort | Huan Ge |
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| description | Summer high temperatures (27–38 °C, more than 7 days) readily induce heat stress in late-laying broiler breeders, which impedes offspring growth and development. This study aimed to provide a scientific basis for improving the offspring development of late-laying broiler breeders during summer. Six hundred fertilized eggs from 50-week-old LiFeng broiler breeders were divided into five treatment groups (non-injected, NaCl-injected, 6 mg/egg L-arginine-injected, 3.5 mg/egg L-methionine-injected, 8.4 mg/egg L-leucine-injected), with six replicates per group and 20 eggs per replicate. Embryos were incubated for 21 days and chicks raised for 21 days post-hatch. Methionine injection significantly enhanced hatchability (+5.8%), increased daily chick weight gain (+8.8%), reduced serum urea nitrogen (−53.13%), decreased inflammatory cytokine levels, elevated antioxidant enzyme activities, lowered malondialdehyde content (−47.99%), and suppressed expression of inflammatory and apoptotic pathway genes. The comprehensive effect of methionine was the best among the three amino acids when injected into chicken embryos. Methionine promoted protein synthesis, enhanced antioxidant and anti-inflammatory capacities, and consequently improved chick growth performance. |
| format | Article |
| id | doaj-art-714a560ce14446d6bf4537c29a0e4b0e |
| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-714a560ce14446d6bf4537c29a0e4b0e2025-08-20T02:35:49ZengMDPI AGAnimals2076-26152025-06-011513193010.3390/ani15131930In Ovo Feeding of Arginine, Leucine, and Methionine in Broiler Breeders’ Eggs During Summer: Effects on Hatchability and Chick Oxidation, Inflammation, and ApoptosisHuan Ge0Zhenwu Huang1Jinghai Feng2Minhong Zhang3State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaSummer high temperatures (27–38 °C, more than 7 days) readily induce heat stress in late-laying broiler breeders, which impedes offspring growth and development. This study aimed to provide a scientific basis for improving the offspring development of late-laying broiler breeders during summer. Six hundred fertilized eggs from 50-week-old LiFeng broiler breeders were divided into five treatment groups (non-injected, NaCl-injected, 6 mg/egg L-arginine-injected, 3.5 mg/egg L-methionine-injected, 8.4 mg/egg L-leucine-injected), with six replicates per group and 20 eggs per replicate. Embryos were incubated for 21 days and chicks raised for 21 days post-hatch. Methionine injection significantly enhanced hatchability (+5.8%), increased daily chick weight gain (+8.8%), reduced serum urea nitrogen (−53.13%), decreased inflammatory cytokine levels, elevated antioxidant enzyme activities, lowered malondialdehyde content (−47.99%), and suppressed expression of inflammatory and apoptotic pathway genes. The comprehensive effect of methionine was the best among the three amino acids when injected into chicken embryos. Methionine promoted protein synthesis, enhanced antioxidant and anti-inflammatory capacities, and consequently improved chick growth performance.https://www.mdpi.com/2076-2615/15/13/1930summerlate laying periodbroiler chickenin ovo feedingmethioninearginine |
| spellingShingle | Huan Ge Zhenwu Huang Jinghai Feng Minhong Zhang In Ovo Feeding of Arginine, Leucine, and Methionine in Broiler Breeders’ Eggs During Summer: Effects on Hatchability and Chick Oxidation, Inflammation, and Apoptosis Animals summer late laying period broiler chicken in ovo feeding methionine arginine |
| title | In Ovo Feeding of Arginine, Leucine, and Methionine in Broiler Breeders’ Eggs During Summer: Effects on Hatchability and Chick Oxidation, Inflammation, and Apoptosis |
| title_full | In Ovo Feeding of Arginine, Leucine, and Methionine in Broiler Breeders’ Eggs During Summer: Effects on Hatchability and Chick Oxidation, Inflammation, and Apoptosis |
| title_fullStr | In Ovo Feeding of Arginine, Leucine, and Methionine in Broiler Breeders’ Eggs During Summer: Effects on Hatchability and Chick Oxidation, Inflammation, and Apoptosis |
| title_full_unstemmed | In Ovo Feeding of Arginine, Leucine, and Methionine in Broiler Breeders’ Eggs During Summer: Effects on Hatchability and Chick Oxidation, Inflammation, and Apoptosis |
| title_short | In Ovo Feeding of Arginine, Leucine, and Methionine in Broiler Breeders’ Eggs During Summer: Effects on Hatchability and Chick Oxidation, Inflammation, and Apoptosis |
| title_sort | in ovo feeding of arginine leucine and methionine in broiler breeders eggs during summer effects on hatchability and chick oxidation inflammation and apoptosis |
| topic | summer late laying period broiler chicken in ovo feeding methionine arginine |
| url | https://www.mdpi.com/2076-2615/15/13/1930 |
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