Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR
Purpose. Up to 30% of international travelers are affected by travelers’ diarrhea (TD). Reliable data on the etiology of TD is lacking. Sufficient laboratory capacity at travel destinations is often unavailable and transporting conventional stool samples to the home country is inconvenient. We evalu...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/3472537 |
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| author | Martin Alberer Nicklas Schlenker Malkin Bauer Kerstin Helfrich Carolin Mengele Thomas Löscher Hans Dieter Nothdurft Gisela Bretzel Marcus Beissner |
| author_facet | Martin Alberer Nicklas Schlenker Malkin Bauer Kerstin Helfrich Carolin Mengele Thomas Löscher Hans Dieter Nothdurft Gisela Bretzel Marcus Beissner |
| author_sort | Martin Alberer |
| collection | DOAJ |
| description | Purpose. Up to 30% of international travelers are affected by travelers’ diarrhea (TD). Reliable data on the etiology of TD is lacking. Sufficient laboratory capacity at travel destinations is often unavailable and transporting conventional stool samples to the home country is inconvenient. We evaluated the use of Hemoccult cards for stool sampling combined with a multiplex PCR for the detection of model viral, bacterial, and protozoal TD pathogens. Methods. Following the creation of serial dilutions for each model pathogen, last positive dilution steps (LPDs) and thereof calculated last positive sample concentrations (LPCs) were compared between conventional stool samples and card samples. Furthermore, card samples were tested after a prolonged time interval simulating storage during a travel duration of up to 6 weeks. Results. The LPDs/LPCs were comparable to testing of conventional stool samples. After storage on Hemoccult cards, the recovery rate was 97.6% for C. jejuni, 100% for E. histolytica, 97.6% for norovirus GI, and 100% for GII. Detection of expected pathogens was possible at weekly intervals up to 42 days. Conclusion. Stool samples on Hemoccult cards stored at room temperature can be used in combination with a multiplex PCR as a reliable tool for testing of TD pathogens. |
| format | Article |
| id | doaj-art-7140fb3a66bd4212b9c5f26f1fc0acfb |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-7140fb3a66bd4212b9c5f26f1fc0acfb2025-02-03T06:45:26ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932017-01-01201710.1155/2017/34725373472537Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCRMartin Alberer0Nicklas Schlenker1Malkin Bauer2Kerstin Helfrich3Carolin Mengele4Thomas Löscher5Hans Dieter Nothdurft6Gisela Bretzel7Marcus Beissner8Department of Infectious Diseases and Tropical Medicine, Medical Centre, Ludwig-Maximilians-University (LMU), Munich, GermanyDepartment of Infectious Diseases and Tropical Medicine, Medical Centre, Ludwig-Maximilians-University (LMU), Munich, GermanyDepartment of Infectious Diseases and Tropical Medicine, Medical Centre, Ludwig-Maximilians-University (LMU), Munich, GermanyDepartment of Infectious Diseases and Tropical Medicine, Medical Centre, Ludwig-Maximilians-University (LMU), Munich, GermanyDepartment of Infectious Diseases and Tropical Medicine, Medical Centre, Ludwig-Maximilians-University (LMU), Munich, GermanyDepartment of Infectious Diseases and Tropical Medicine, Medical Centre, Ludwig-Maximilians-University (LMU), Munich, GermanyDepartment of Infectious Diseases and Tropical Medicine, Medical Centre, Ludwig-Maximilians-University (LMU), Munich, GermanyDepartment of Infectious Diseases and Tropical Medicine, Medical Centre, Ludwig-Maximilians-University (LMU), Munich, GermanyDepartment of Infectious Diseases and Tropical Medicine, Medical Centre, Ludwig-Maximilians-University (LMU), Munich, GermanyPurpose. Up to 30% of international travelers are affected by travelers’ diarrhea (TD). Reliable data on the etiology of TD is lacking. Sufficient laboratory capacity at travel destinations is often unavailable and transporting conventional stool samples to the home country is inconvenient. We evaluated the use of Hemoccult cards for stool sampling combined with a multiplex PCR for the detection of model viral, bacterial, and protozoal TD pathogens. Methods. Following the creation of serial dilutions for each model pathogen, last positive dilution steps (LPDs) and thereof calculated last positive sample concentrations (LPCs) were compared between conventional stool samples and card samples. Furthermore, card samples were tested after a prolonged time interval simulating storage during a travel duration of up to 6 weeks. Results. The LPDs/LPCs were comparable to testing of conventional stool samples. After storage on Hemoccult cards, the recovery rate was 97.6% for C. jejuni, 100% for E. histolytica, 97.6% for norovirus GI, and 100% for GII. Detection of expected pathogens was possible at weekly intervals up to 42 days. Conclusion. Stool samples on Hemoccult cards stored at room temperature can be used in combination with a multiplex PCR as a reliable tool for testing of TD pathogens.http://dx.doi.org/10.1155/2017/3472537 |
| spellingShingle | Martin Alberer Nicklas Schlenker Malkin Bauer Kerstin Helfrich Carolin Mengele Thomas Löscher Hans Dieter Nothdurft Gisela Bretzel Marcus Beissner Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR |
| title_full | Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR |
| title_fullStr | Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR |
| title_full_unstemmed | Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR |
| title_short | Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR |
| title_sort | detection of gastrointestinal pathogens from stool samples on hemoccult cards by multiplex pcr |
| url | http://dx.doi.org/10.1155/2017/3472537 |
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