Revealing the mechanisms and therapeutic potential of immune checkpoint proteins across diverse protein families

Host immune responses to antigens are tightly regulated through the activation and inhibition of synergistic signaling networks that maintain homeostasis. Stimulatory checkpoint molecules initiate attacks on infected or tumor cells, while inhibitory molecules halt the immune response to prevent over...

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Main Authors: Ran Liu, Xinyan Jiang, Ruijuan Dong, Yuting Zhang, Cong Gai, Peng Wei
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Immunology
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Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2025.1499663/full
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author Ran Liu
Xinyan Jiang
Ruijuan Dong
Yuting Zhang
Cong Gai
Peng Wei
author_facet Ran Liu
Xinyan Jiang
Ruijuan Dong
Yuting Zhang
Cong Gai
Peng Wei
author_sort Ran Liu
collection DOAJ
description Host immune responses to antigens are tightly regulated through the activation and inhibition of synergistic signaling networks that maintain homeostasis. Stimulatory checkpoint molecules initiate attacks on infected or tumor cells, while inhibitory molecules halt the immune response to prevent overreaction and self-injury. Multiple immune checkpoint proteins are grouped into families based on common structural domains or origins, yet the variability within and between these families remains largely unexplored. In this review, we discuss the current understanding of the mechanisms underlying the co-suppressive functions of CTLA-4, PD-1, and other prominent immune checkpoint pathways. Additionally, we examine the IgSF, PVR, TIM, SIRP, and TNF families, including key members such as TIGIT, LAG-3, VISTA, TIM-3, SIRPα, and OX40. We also highlight the unique dual role of VISTA and SIRPα in modulating immune responses under specific conditions, and explore potential immunotherapeutic pathways tailored to the distinct characteristics of different immune checkpoint proteins. These insights into the unique advantages of checkpoint proteins provide new directions for drug discovery, emphasizing that emerging immune checkpoint molecules could serve as targets for novel therapies in cancer, autoimmune diseases, infectious diseases, and transplant rejection.
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spelling doaj-art-7138383af8d54dbdaa17ea9a38ccfaf22025-08-20T02:19:58ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-04-011610.3389/fimmu.2025.14996631499663Revealing the mechanisms and therapeutic potential of immune checkpoint proteins across diverse protein familiesRan LiuXinyan JiangRuijuan DongYuting ZhangCong GaiPeng WeiHost immune responses to antigens are tightly regulated through the activation and inhibition of synergistic signaling networks that maintain homeostasis. Stimulatory checkpoint molecules initiate attacks on infected or tumor cells, while inhibitory molecules halt the immune response to prevent overreaction and self-injury. Multiple immune checkpoint proteins are grouped into families based on common structural domains or origins, yet the variability within and between these families remains largely unexplored. In this review, we discuss the current understanding of the mechanisms underlying the co-suppressive functions of CTLA-4, PD-1, and other prominent immune checkpoint pathways. Additionally, we examine the IgSF, PVR, TIM, SIRP, and TNF families, including key members such as TIGIT, LAG-3, VISTA, TIM-3, SIRPα, and OX40. We also highlight the unique dual role of VISTA and SIRPα in modulating immune responses under specific conditions, and explore potential immunotherapeutic pathways tailored to the distinct characteristics of different immune checkpoint proteins. These insights into the unique advantages of checkpoint proteins provide new directions for drug discovery, emphasizing that emerging immune checkpoint molecules could serve as targets for novel therapies in cancer, autoimmune diseases, infectious diseases, and transplant rejection.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1499663/fullimmunotherapyimmune checkpoint proteinstumor microenvironment specificityco-suppressive pathwaysprotein families
spellingShingle Ran Liu
Xinyan Jiang
Ruijuan Dong
Yuting Zhang
Cong Gai
Peng Wei
Revealing the mechanisms and therapeutic potential of immune checkpoint proteins across diverse protein families
Frontiers in Immunology
immunotherapy
immune checkpoint proteins
tumor microenvironment specificity
co-suppressive pathways
protein families
title Revealing the mechanisms and therapeutic potential of immune checkpoint proteins across diverse protein families
title_full Revealing the mechanisms and therapeutic potential of immune checkpoint proteins across diverse protein families
title_fullStr Revealing the mechanisms and therapeutic potential of immune checkpoint proteins across diverse protein families
title_full_unstemmed Revealing the mechanisms and therapeutic potential of immune checkpoint proteins across diverse protein families
title_short Revealing the mechanisms and therapeutic potential of immune checkpoint proteins across diverse protein families
title_sort revealing the mechanisms and therapeutic potential of immune checkpoint proteins across diverse protein families
topic immunotherapy
immune checkpoint proteins
tumor microenvironment specificity
co-suppressive pathways
protein families
url https://www.frontiersin.org/articles/10.3389/fimmu.2025.1499663/full
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AT xinyanjiang revealingthemechanismsandtherapeuticpotentialofimmunecheckpointproteinsacrossdiverseproteinfamilies
AT ruijuandong revealingthemechanismsandtherapeuticpotentialofimmunecheckpointproteinsacrossdiverseproteinfamilies
AT yutingzhang revealingthemechanismsandtherapeuticpotentialofimmunecheckpointproteinsacrossdiverseproteinfamilies
AT conggai revealingthemechanismsandtherapeuticpotentialofimmunecheckpointproteinsacrossdiverseproteinfamilies
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