Green synthesis of silver nanoparticles using Nigella sativa with 0.1% of thymoquinone and evaluation of its anti-inflammatory and antioxidant activities – An in vitro study
Background: The therapeutic potential of herbs in treating various diseases is gaining recognition due to their promising outcomes and minimal side effects. One such herb, Nigella sativa, is renowned for its medicinal properties, particularly due to its extract, Thymoquinone (TQ), which demonstrates...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-01-01
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| Series: | Journal of Indian Society of Periodontology |
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| Online Access: | https://journals.lww.com/10.4103/jisp.jisp_278_24 |
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| author | Leslie Rani Sthanislas Bagavad Gita Jayaraman Gautham Kumar Nandhakumar C. S. Krishnan Gowri Shankar Nattuthurai Menaka Vasudevan Savithri Nediyaparambu Krishnakumar Noblin Shyma |
| author_facet | Leslie Rani Sthanislas Bagavad Gita Jayaraman Gautham Kumar Nandhakumar C. S. Krishnan Gowri Shankar Nattuthurai Menaka Vasudevan Savithri Nediyaparambu Krishnakumar Noblin Shyma |
| author_sort | Leslie Rani Sthanislas |
| collection | DOAJ |
| description | Background:
The therapeutic potential of herbs in treating various diseases is gaining recognition due to their promising outcomes and minimal side effects. One such herb, Nigella sativa, is renowned for its medicinal properties, particularly due to its extract, Thymoquinone (TQ), which demonstrates anti-inflammatory, anti-oxidant, antimicrobial, analgesic, and immune-modulating effects. Green synthesis methods for nano-sized particles are considered environmentally friendly and have been proven effective given their size, higher surface-to-volume ratio, and enhanced physical and mechanical properties. This study aims to investigate a novel approach in the green synthesis of silver nanoparticles of Nigella sativa and 0.1% TQ and to assess their anti-inflammatory and antioxidant activities
. Materials and Methods:
The seeds of NS were used to green synthesize silver nanoparticles. The anti-inflammatory effects of the green synthesized particles were tested using egg albumin and bovine serum assays, and their anti-oxidant actions were assessed using 2,2-Diphenyl-1-picrylhydrazyl denaturation assay and H2O2 assay.
Results:
The novel formulation of Nigella sativa-mediated silver nanoparticles with 0.1% TQ exhibits 76% anti-inflammatory activity and 80%–87% anti-oxidant properties.
Conclusion:
Our results suggest that the silver nanoparticles mediated from N. sativa with 0.1% TQ extract could serve as an effective herbal adjunct for treating periodontal infections because of its potent anti-inflammatory and anti-oxidant properties. |
| format | Article |
| id | doaj-art-71354d9eac7d4fa0bfff4d797a488b34 |
| institution | Kabale University |
| issn | 0972-124X 0975-1580 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Society of Periodontology |
| spelling | doaj-art-71354d9eac7d4fa0bfff4d797a488b342025-08-20T03:31:24ZengWolters Kluwer Medknow PublicationsJournal of Indian Society of Periodontology0972-124X0975-15802025-01-01291576210.4103/jisp.jisp_278_24Green synthesis of silver nanoparticles using Nigella sativa with 0.1% of thymoquinone and evaluation of its anti-inflammatory and antioxidant activities – An in vitro studyLeslie Rani SthanislasBagavad Gita JayaramanGautham Kumar NandhakumarC. S. KrishnanGowri Shankar NattuthuraiMenaka VasudevanSavithri Nediyaparambu KrishnakumarNoblin ShymaBackground: The therapeutic potential of herbs in treating various diseases is gaining recognition due to their promising outcomes and minimal side effects. One such herb, Nigella sativa, is renowned for its medicinal properties, particularly due to its extract, Thymoquinone (TQ), which demonstrates anti-inflammatory, anti-oxidant, antimicrobial, analgesic, and immune-modulating effects. Green synthesis methods for nano-sized particles are considered environmentally friendly and have been proven effective given their size, higher surface-to-volume ratio, and enhanced physical and mechanical properties. This study aims to investigate a novel approach in the green synthesis of silver nanoparticles of Nigella sativa and 0.1% TQ and to assess their anti-inflammatory and antioxidant activities . Materials and Methods: The seeds of NS were used to green synthesize silver nanoparticles. The anti-inflammatory effects of the green synthesized particles were tested using egg albumin and bovine serum assays, and their anti-oxidant actions were assessed using 2,2-Diphenyl-1-picrylhydrazyl denaturation assay and H2O2 assay. Results: The novel formulation of Nigella sativa-mediated silver nanoparticles with 0.1% TQ exhibits 76% anti-inflammatory activity and 80%–87% anti-oxidant properties. Conclusion: Our results suggest that the silver nanoparticles mediated from N. sativa with 0.1% TQ extract could serve as an effective herbal adjunct for treating periodontal infections because of its potent anti-inflammatory and anti-oxidant properties.https://journals.lww.com/10.4103/jisp.jisp_278_24anti-inflammatoryanti-oxidantgreen synthesisnanotechnologyperiodontitisthymoquinone |
| spellingShingle | Leslie Rani Sthanislas Bagavad Gita Jayaraman Gautham Kumar Nandhakumar C. S. Krishnan Gowri Shankar Nattuthurai Menaka Vasudevan Savithri Nediyaparambu Krishnakumar Noblin Shyma Green synthesis of silver nanoparticles using Nigella sativa with 0.1% of thymoquinone and evaluation of its anti-inflammatory and antioxidant activities – An in vitro study Journal of Indian Society of Periodontology anti-inflammatory anti-oxidant green synthesis nanotechnology periodontitis thymoquinone |
| title | Green synthesis of silver nanoparticles using Nigella sativa with 0.1% of thymoquinone and evaluation of its anti-inflammatory and antioxidant activities – An in vitro study |
| title_full | Green synthesis of silver nanoparticles using Nigella sativa with 0.1% of thymoquinone and evaluation of its anti-inflammatory and antioxidant activities – An in vitro study |
| title_fullStr | Green synthesis of silver nanoparticles using Nigella sativa with 0.1% of thymoquinone and evaluation of its anti-inflammatory and antioxidant activities – An in vitro study |
| title_full_unstemmed | Green synthesis of silver nanoparticles using Nigella sativa with 0.1% of thymoquinone and evaluation of its anti-inflammatory and antioxidant activities – An in vitro study |
| title_short | Green synthesis of silver nanoparticles using Nigella sativa with 0.1% of thymoquinone and evaluation of its anti-inflammatory and antioxidant activities – An in vitro study |
| title_sort | green synthesis of silver nanoparticles using nigella sativa with 0 1 of thymoquinone and evaluation of its anti inflammatory and antioxidant activities an in vitro study |
| topic | anti-inflammatory anti-oxidant green synthesis nanotechnology periodontitis thymoquinone |
| url | https://journals.lww.com/10.4103/jisp.jisp_278_24 |
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