ASFV infection induces lipid metabolic disturbances and promotes viral replication
IntroductionAfrican swine fever is a highly transmissible and lethal infectious disease caused by the African swine fever virus (ASFV), which has considerably impacted the global swine industry. Lipid metabolism plays a vital role in sustaining lipid and energy homeostasis within cells and influence...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1532678/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841543272146665472 |
|---|---|
| author | Xuefei Chu Xuefei Chu Shengqiang Ge Shengqiang Ge Shengqiang Ge Yingchao Li Qin Zhang Xinyu Cui Yuanyuan Zuo Ruihong Li Hongtao Sun Lei Yin Zhenzhong Wang Jinming Li Yihong Xiao Zhiliang Wang Zhiliang Wang |
| author_facet | Xuefei Chu Xuefei Chu Shengqiang Ge Shengqiang Ge Shengqiang Ge Yingchao Li Qin Zhang Xinyu Cui Yuanyuan Zuo Ruihong Li Hongtao Sun Lei Yin Zhenzhong Wang Jinming Li Yihong Xiao Zhiliang Wang Zhiliang Wang |
| author_sort | Xuefei Chu |
| collection | DOAJ |
| description | IntroductionAfrican swine fever is a highly transmissible and lethal infectious disease caused by the African swine fever virus (ASFV), which has considerably impacted the global swine industry. Lipid metabolism plays a vital role in sustaining lipid and energy homeostasis within cells and influences the viral life cycle.Methods and resultsIn this study, we found that ASFV infection disrupts lipid metabolism in the host. Transcriptomic analysis of cells infected with ASFV revealed that the levels of lipid metabolism significantly changed as the duration of the infection progressed. The intracellular cholesterol levels of the host exhibited a pattern similar to the viral growth curve during the course of infection. Notably, increased cholesterol levels promoted ASFV replication in host cells, whereas inhibition of the cholesterol biosynthesis pathway markedly reduced intracellular ASFV replication.DiscussionThe findings of this study showed that ASFV led to lipid metabolism disturbances to facilitate its replication, which is useful for revealing the mechanism underlying ASFV infection. |
| format | Article |
| id | doaj-art-7134f55c66a142249f34024a446ad807 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-7134f55c66a142249f34024a446ad8072025-01-13T15:24:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.15326781532678ASFV infection induces lipid metabolic disturbances and promotes viral replicationXuefei Chu0Xuefei Chu1Shengqiang Ge2Shengqiang Ge3Shengqiang Ge4Yingchao Li5Qin Zhang6Xinyu Cui7Yuanyuan Zuo8Ruihong Li9Hongtao Sun10Lei Yin11Zhenzhong Wang12Jinming Li13Yihong Xiao14Zhiliang Wang15Zhiliang Wang16College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, ChinaChina Animal Health and Epidemiology Center, Qingdao, ChinaChina Animal Health and Epidemiology Center, Qingdao, ChinaQingdao Key Laboratory of Modern Bioengineering and Animal Disease Research, Qingdao, ChinaKey Laboratory of Animal Biosafety Risk Warning Prevention and Control (South China), Ministry of Agriculture and Rural Affairs, Qingdao, Shandong, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, ChinaChina Animal Health and Epidemiology Center, Qingdao, ChinaChina Animal Health and Epidemiology Center, Qingdao, ChinaChina Animal Health and Epidemiology Center, Qingdao, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong, ChinaChina Animal Health and Epidemiology Center, Qingdao, ChinaChina Animal Health and Epidemiology Center, Qingdao, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, ChinaChina Animal Health and Epidemiology Center, Qingdao, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong, ChinaIntroductionAfrican swine fever is a highly transmissible and lethal infectious disease caused by the African swine fever virus (ASFV), which has considerably impacted the global swine industry. Lipid metabolism plays a vital role in sustaining lipid and energy homeostasis within cells and influences the viral life cycle.Methods and resultsIn this study, we found that ASFV infection disrupts lipid metabolism in the host. Transcriptomic analysis of cells infected with ASFV revealed that the levels of lipid metabolism significantly changed as the duration of the infection progressed. The intracellular cholesterol levels of the host exhibited a pattern similar to the viral growth curve during the course of infection. Notably, increased cholesterol levels promoted ASFV replication in host cells, whereas inhibition of the cholesterol biosynthesis pathway markedly reduced intracellular ASFV replication.DiscussionThe findings of this study showed that ASFV led to lipid metabolism disturbances to facilitate its replication, which is useful for revealing the mechanism underlying ASFV infection.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1532678/fullAfrican swine fever viruslipid metabolismcholesterollipid synthesistranscriptomic analysis |
| spellingShingle | Xuefei Chu Xuefei Chu Shengqiang Ge Shengqiang Ge Shengqiang Ge Yingchao Li Qin Zhang Xinyu Cui Yuanyuan Zuo Ruihong Li Hongtao Sun Lei Yin Zhenzhong Wang Jinming Li Yihong Xiao Zhiliang Wang Zhiliang Wang ASFV infection induces lipid metabolic disturbances and promotes viral replication Frontiers in Microbiology African swine fever virus lipid metabolism cholesterol lipid synthesis transcriptomic analysis |
| title | ASFV infection induces lipid metabolic disturbances and promotes viral replication |
| title_full | ASFV infection induces lipid metabolic disturbances and promotes viral replication |
| title_fullStr | ASFV infection induces lipid metabolic disturbances and promotes viral replication |
| title_full_unstemmed | ASFV infection induces lipid metabolic disturbances and promotes viral replication |
| title_short | ASFV infection induces lipid metabolic disturbances and promotes viral replication |
| title_sort | asfv infection induces lipid metabolic disturbances and promotes viral replication |
| topic | African swine fever virus lipid metabolism cholesterol lipid synthesis transcriptomic analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1532678/full |
| work_keys_str_mv | AT xuefeichu asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT xuefeichu asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT shengqiangge asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT shengqiangge asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT shengqiangge asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT yingchaoli asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT qinzhang asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT xinyucui asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT yuanyuanzuo asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT ruihongli asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT hongtaosun asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT leiyin asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT zhenzhongwang asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT jinmingli asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT yihongxiao asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT zhiliangwang asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication AT zhiliangwang asfvinfectioninduceslipidmetabolicdisturbancesandpromotesviralreplication |